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anti-Human LAT antibody for Flow Cytometry

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
Epitope
Cytoplasmic Domain
29
18
17
15
11
9
9
7
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
240
104
91
2
1
1
1
1
Host
Mouse
137
111
14
2
Clonality
Monoclonal
Conjugate
This LAT antibody is un-conjugated
9
9
8
8
8
6
5
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
193
69
58
51
50
33
23
20
16
6
2
2
2
2
1
1
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Catalog No. ABIN302069
$ 265.65
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN2662791 FACS PE Mouse IgG1, IgG1 kappa Log in to see LAT1111 4
0.0010482287 ABIN120717 FACS IP WB Mouse IgG1 Log in to see LAT-01
0.0010482287 ABIN2666055 FACS Mouse IgG1 kappa Log in to see 11B-12 4
0.0010482287 ABIN207036 FACS IP WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT-01
0.0010482287 ABIN2662792 FACS PE Mouse IgG1, IgG1 kappa Log in to see LAT1111 4
0.0010482287 ABIN2666056 FACS Mouse IgG1 kappa Log in to see 11B-12 4
0.0010482287 ABIN269134 FACS IP WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT-01
0.0010482287 ABIN4330385 FACS Alexa Fluor 488 Mouse IgG2b AA 30-113 Log in to see 661002
0.0010482287 ABIN4330386 FACS Alexa Fluor 700 Mouse IgG2b AA 30-113 Log in to see 661002
0.0010482287 ABIN4330387 FACS Alexa Fluor 647 Mouse IgG2b AA 30-113 Log in to see 661002
0.0010482287 ABIN4330388 FACS Alexa Fluor 750 Mouse IgG2b AA 30-113 Log in to see 661002
0.0010482287 ABIN4330389 FACS Alexa Fluor 594 Mouse IgG2b AA 30-113 Log in to see 661002
0.0010482287 ABIN4330390 FACS Alexa Fluor 350 Mouse IgG2b AA 30-113 Log in to see 661002
0.0010482287 ABIN4330391 FACS Alexa Fluor 405 Mouse IgG2b AA 30-113 Log in to see 661002
0.0010482287 ABIN596255 FACS Mouse IgG1 Log in to see
0.0010482287 ABIN4330367 FACS IP WB DyLight 350 Mouse IgG1 Cytoplasmic Domain Log in to see LAT-01
0.0010482287 ABIN4330369 FACS IP WB DyLight 405 Mouse IgG1 Cytoplasmic Domain Log in to see LAT-01
0.0010482287 ABIN4330371 FACS IP WB PE Mouse IgG1 Cytoplasmic Domain Log in to see LAT-01
0.0010482287 ABIN4330373 FACS IP WB PerCP Mouse IgG1 Cytoplasmic Domain Log in to see LAT-01
0.0010482287 ABIN4330375 FACS IP WB Allophycocyanin Mouse IgG1 Cytoplasmic Domain Log in to see LAT-01

Top referenced anti-LAT antibody for Flow Cytometry

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Application / Reactivity Human
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ELISA 66 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 53 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 20 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies
Immunoprecipitation (IP) 41 Antibodies
Intracellular Staining (ICS) 6 Antibodies
Western Blotting (WB) 172 Antibodies

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Epitope Cytoplasmic Domain
(29), (18), (17), (15), (11), (9), (9), (7), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(240), (104), (91), (2), (1), (1), (1), (1)
Host Mouse
(137), (111), (14), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LAT antibody is un-conjugated
(9), (9), (8), (8), (8), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(193), (69), (58), (51), (50), (33), (23), (20), (16), (6), (2), (2), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-LAT Antibody

Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN302069) Images
Specificity The antibody LAT-01 reacts with Linker for activation of T cells (LAT), a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
No Cross-Reactivity Mouse (Murine)
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT.
Clone LAT-01
Isotype IgG1

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling References for anti-LAT Antibody (ABIN302069) Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
Research Area Signaling, Adaptive Immunity
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT Antibody (ABIN302069) Images back to top
Application Notes Immunoprecipitation:
Application note: Tyrosine-phosphorylated form of LAT is not optimally recognized.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT Antibody (ABIN302069) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-LAT Antibody (ABIN302069)

Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Product cited in:

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011

Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, 2005

Background publications

Yamasaki, Ishikawa, Sakuma, Kanagawa, Cheng, Malissen, Saito: "LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4406-15, 2007

Simeoni, Smida, Posevitz, Schraven, Lindquist: "Right time, right place: the organization of membrane proximal signaling." in: Seminars in immunology, Vol. 17, Issue 1, pp. 35-49, 2004

Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, 2004

Images

Product Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN302069) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-LAT antibody (Linker For Activation of T Cells) (Cytoplasmic Domain) (ABIN302069) Flow Cytometry analysis of LAT expression in peripheral blood lymphocytes using anti-...