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anti-Human LAT antibody for Intracellular Staining

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
Epitope
pTyr171
29
19
17
15
11
9
8
7
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
240
104
91
2
1
1
1
1
Host
Mouse
137
111
14
2
Clonality
Monoclonal
Conjugate
This LAT antibody is conjugated to PE
9
9
8
8
7
6
5
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
1
1
1
1
Application
Intracellular Staining (ICS)
194
69
59
52
50
33
23
20
16
5
2
2
2
2
1
1
Supplier
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Catalog No. ABIN1177079
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN1177080 ICS PE Mouse IgG1, kappa pTyr226 Log in to see J96-1238-58-93 5
0.0008709465 ABIN1177077 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr171 Log in to see I58-1169 6
0.0008709465 ABIN1177078 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr171 Log in to see I58-1169 6
0.0008709465 ABIN1177082 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr226 Log in to see J96-1238-58-93 5
0.0008709465 ABIN1177081 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr226 Log in to see J96-1238-58-93 2

Top referenced anti-LAT antibody for Intracellular Staining

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Flow Cytometry (FACS) 53 Antibodies
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Western Blotting (WB) 172 Antibodies

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Epitope pTyr171
(29), (19), (17), (15), (11), (9), (8), (7), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(240), (104), (91), (2), (1), (1), (1), (1)
Host Mouse
(137), (111), (14), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LAT antibody is conjugated to PE
(9), (9), (8), (8), (7), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Application Intracellular Staining (ICS)
(194), (69), (59), (52), (50), (33), (23), (20), (16), (5), (2), (2), (2), (2), (1), (1)
Pubmed 6 references available
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Product Details anti-LAT Antibody

Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN1177079) Images
Brand BD Phosflow™
Purification Purified from tissue culture supernatant or ascites by affinity chromatography
Immunogen Phosphorylated Human LAT
Clone I58-1169
Isotype IgG1, kappa

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling References for anti-LAT Antibody (ABIN1177079) Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background Engagement of the T cell receptor (TCR) induces signal transduction pathways that enhance gene transcription and cellular proliferation and differentiation. TCR ligation results in the recruitment and activation of multiple protein tyrosine kinases (PTKs), including lck, fyn, and ZAP70. Adaptor proteins, such as Grb2 and SLP-76, relay the signal to downstream effector molecules. LAT (linker for activation of T cells) is a substrate of the activated ZAP70 and functions to bridge the activated TCR and its associated PTKs with tyrosine kinase substrates. LAT is expressed as 36- and 38-kDa forms that result from post-translational modification, and as a 42-kDa form that results from alternative splicing. LAT is an integral membrane protein that is phosphorylated at five tyrosine sites upon TCR ligation. Following phosphorylation, LAT binds a number of important signaling molecules, including Grb2, Vav, PLCgamma1, and the p85 subunit of PI3K. Multiple studies have shown that functional LAT is required for T lymphocyte activation and thymocyte development. The I58-1169 monoclonal antibody recognizes the phosphorylated tyrosine 171 (pY171) of LAT, which is one of the phosphotyrosine sites required for binding phosphoinositide 3-kinase, Grb2, and Gads.
Research Area Adaptive Immunity
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT Antibody (ABIN1177079) Images back to top
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT Antibody (ABIN1177079) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-LAT Antibody (ABIN1177079)

Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Product cited in:

Cho, Velikovsky, Swaminathan, Houtman, Samelson, Mariuzza: "Structural basis for differential recognition of tyrosine-phosphorylated sites in the linker for activation of T cells (LAT) by the adaptor Gads." in: The EMBO journal, Vol. 23, Issue 7, pp. 1441-51, 2004

Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, 2003

Samelson: "Signal transduction mediated by the T cell antigen receptor: the role of adapter proteins." in: Annual review of immunology, Vol. 20, pp. 371-94, 2002

Zhu, Janssen, Zhang: "Minimal requirement of tyrosine residues of linker for activation of T cells in TCR signaling and thymocyte development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 325-33, 2002

Lin, Weiss: "Identification of the minimal tyrosine residues required for linker for activation of T cell function." in: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 29588-95, 2001

Paz, Wang, Clarke, Lu, Stokoe, Abo: "Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells." in: The Biochemical journal, Vol. 356, Issue Pt 2, pp. 461-71, 2001

Images

Product Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN1177079) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-LAT antibody (Linker For Activation of T Cells) (pTyr171) (PE) (ABIN1177079) anti-Linker For Activation of T Cells (LAT) (pTyr171) antibody (PE)