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|Application / Reactivity||Mouse (Murine)||Rat (Rattus)||Human|
|Western Blotting (WB)||49 Antibodies||42 Antibodies||127 Antibodies|
|Intracellular Staining (ICS)||6 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies||5 Antibodies||25 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||25 Antibodies||11 Antibodies||9 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies||2 Antibodies||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||33 Antibodies||33 Antibodies|
|Antigen||Linker For Activation of T Cells (LAT) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This LAT antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-LAT AntibodyTarget Details LAT Application Details Handling References for anti-LAT Antibody (ABIN374446) Images
|Specificity||This antibody is expected to recognize isoform a (NP_055202.1), isoform b (NP_001014987.1 and NP_001014989.1) and isoform c (NP_001014988.1).|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse and Rat.|
|Purification||Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.|
|Immunogen||Peptide from the C Terminus of the protein sequence according to NP_055202.1, NP_001014987.1, NP_001014989.1, NP_001014988.1|
Target Details LATProduct Details anti-LAT Antibody Application Details Handling References for anti-LAT Antibody (ABIN374446) Images back to top
|Alternative Name||LAT (LAT Antibody Abstract)|
|Background||Linker for activation of T cells (LAT) is an integral membrane protein (36 to 38 kD), which plays an important role in linking engagement of the TCR to the biochemical events of T cell activation. LAT antibody stains thymus and peripheral lymphoid tissues such as T cell areas in lymph nodes and spleen. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. LAT is not expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells.Synonyms: 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-Cells Family Member 1, p36-38, pp36|
|Molecular Weight||27.9kDa (NP_055202.1).|
|Research Area||Signaling, Adaptive Immunity|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT Antibody (ABIN374446) Images back to top
Peptide ELISA: 1/2,000. Western Blot: 1-3 μg/mL. Detects a band of Approx 35 kDa in Human PBMC lysates(Predicted Molecular Weight: 35 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT Antibody (ABIN374446) Images back to top
|Buffer||Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody (in aliquots) at -20 °C.|
References for anti-LAT Antibody (ABIN374446)Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Finco, Kadlecek, Zhang et al.: "LAT is required for TCR-mediated activation of PLCgamma1 and the Ras pathway." in: Immunity, Vol. 9, Issue 5, pp. 617-26, 1998 (PubMed).
ImagesProduct Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN374446) back to top