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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)||Human|
|Dot Blot (DB)||2 Antibodies||2 Antibodies||2 Antibodies|
|ELISA||6 Antibodies||3 Antibodies||38 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies||12 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies|
|Immunohistochemistry (IHC)||1 Antibodies||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies|
|Western Blotting (WB)||6 Antibodies||5 Antibodies|
|Detection (D)||1 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Antigen||Luteinizing Hormone beta Polypeptide (LHB) Antibodies|
|Epitope||AA 21-40, N-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This LHB antibody is un-conjugated Alternatives|
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
|1 reference available|
|Supplier||Log in to see|
Product Details anti-LHB AntibodyTarget Details LHB Application Details Handling References for anti-LHB Antibody (ABIN493089) Images
|Sequence||RPW CHP INA ILA VEK EGC PV|
|Specificity||This antibody labels gonadotrophs in pituitary sections. It does not react to other cellular proteins that are tested during characterization of this antibody.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse, Rat.|
|Immunogen||Synthetic peptide corresponding to 21-40 amino acids of Human LH|
Target Details LHBProduct Details anti-LHB Antibody Application Details Handling References for anti-LHB Antibody (ABIN493089) Images back to top
|Alternative Name||Luteinizing Hormone beta (LSH-Beta) (LHB Antibody Abstract)|
|Background||The pituitary hormones, luteinizing hormone (lutropin, LH) and follicle stimulating hormone (follitropin, FSH) as well as related placental hormones, chorionic gonadotropin and CG, are referred to as gonadotropic hormones because of their action on gonad cells. These three hormones are structurally related to each other as well as to thyroid stimulating hormone (TSH). Because of their similar structure and composition and glycoprotein nature, LH, TSH, GC and FSH, these hormones are referred to as glycoprotein hormones. All these hormones are heterodimeric in nature composed of two un-identical subunits, alpha and beta which are non-covalently associated, Among humans and other species, the Alpha subunit is identical for all these hormones and is coded by a single gene, where is beta subunits are distinct but homologous and arise from separate genes. The individual hormone subunits and peptides derived from them have shown some binding activities to their respective receptors, the association of alpha and beta subunits clearly is required for optimal activation and physiological responses elicited by these hormones. Within the heterodimeric hormones, the beta subunit confors the binding activity to its respective receptor. The LH and CG beta subunits have 85 % sequence identity in their first 114 amino acids, the LH/CG receptors can recognize either of the pituitary LH or placental CG hormones. The alpha and beta subunits of LH, CG and FSH are highly glycosylated (1). The alpha subunit has two N-linked oligosdaccharide chain at position 52 and 78 and beta subunits have one in LH and TSH and two in CG and FSH. The CG beta subunit also has an extension of serine O-linked carbohydrates at carboxyl end which help is plasma half like of CG compared to other glycohormone beta subunits. The N-linked oligosaccharides on FSH are sialylated whereas LH and TSH are sialylated and sulfated. There are 10-12 cysteins forming 3 disulfide bonds referred to as Synonyms: LH-B, LHB, LSH-beta, Luteinizing hormone subunit beta, Lutropin beta chain, Lutropin subunit beta|
Application DetailsProduct Details anti-LHB Antibody Target Details LHB Handling References for anti-LHB Antibody (ABIN493089) Images back to top
Dot blot. ELISA: 1/50,000 in dilution buffer. Immunohistochemistry on Frozen Sections: 1/250.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-LHB Antibody Target Details LHB Application Details References for anti-LHB Antibody (ABIN493089) Images back to top
|Buffer||Stabilization buffer with 0.02 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-LHB Antibody (ABIN493089)Product Details anti-LHB Antibody Target Details LHB Application Details Handling Images back to top
Sairam: "Role of carbohydrates in glycoprotein hormone signal transduction." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 3, Issue 8, pp. 1915-26, 1989 (PubMed).