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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||10 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||22 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||33 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||21 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Western Blotting (WB)||81 Antibodies|
|Antigen||Mucosa Associated Lymphoid Tissue Lymphoma Translocation Gene 1 (MALT1) Antibodies|
|Epitope||Internal Region Alternatives|
|Conjugate||This MALT1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-MALT1 AntibodyTarget Details MALT1 Application Details Handling Images
|Predicted Reactivity||Dog (Canine),Mouse (Murine),Rabbit|
|Characteristics||Cellular Localization: Cytoplasm, nucleus|
|Purification||Immunogen Affinity Purified|
|Immunogen||Synthetic peptide derived from internal region of human MALT1 protein.|
Target Details MALT1Product Details anti-MALT1 Antibody Application Details Handling Images back to top
|Alternative Name||MALT-1 (MALT1 Antibody Abstract)|
|Background||MALT-1 was independently identified as a member of the human paracaspase family and an interacting partner of B cell lymphoma. Two alternatively spliced transcript variants encoding different isoforms (a and b) have been described for the MALT-1 gene. Human MALT isoform a, is an amino acid protein of 824 amino acids, and human MALT-2 isoform b, is an amino acid protein of 813 amino acids. MALT-1 is thought to play a role in NFkappaB signaling by enhancing NFkappaB activation through interaction with Bcl-10. Interaction between MALT-1 and Bcl-10 mediates IKK activation in-vitro through facilitating the ubiquitination of NEMO by the ubiquitin conjugating enzyme UBC13.|
|Molecular Weight||90 kDa|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-MALT1 Antibody Target Details MALT1 Handling Images back to top
|Application Notes||IHC Dilution: 1:100|
IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
|Restrictions||For Research Use only|
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|Buffer||immunogen affinity purified rabbit polyclonal antibody in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.|
|Precaution of Use||
1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
|Handling Advice||Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.|
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