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|Application / Reactivity||Rat (Rattus)|
|ELISA||4 ELISA Kits|
|Antigen||Protein Kinase B (PKB) ELISA Kits|
|Reactivity||Rat (Rattus) Alternatives|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
|Detection Range||3.12-200 pg/mL|
|Minimum Detection Limit||3.12 pg/mL|
|Supplier||Log in to see|
Product Details PKB ELISA KitTarget details Application Details Handling Images
|Purpose||For the quantitative determination of rat phosphorylated protein kinase B (p-PKB) concentrations in serum, plasma, tissue homogenates.|
|Sample Type||Serum, Plasma, Tissue Homogenate|
|Specificity||This assay has high sensitivity and excellent specificity for detection of rat p-PKB.|
|Cross-Reactivity (Details)||Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.|
|Material not included||
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|Pathways||TCR Signaling, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation|
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Detection wavelength: 450 nm
|Sample Volume||100 μL|
|Assay Time||1 - 4.5 h|
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for p-PKB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any p-PKB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for p-PKB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of p-PKB bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|Calculation of Results||
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
|Restrictions||For Research Use only|
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|Precaution of Use||The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.|
|Storage||4 °C/-20 °C|
|Storage Comment||May be stored at 2-8°C for up to 1 month. For long term storage, please store at -20°C. Try to keep assay plate in a sealed aluminium foil bag and avoid dampness.|
|Expiry Date||6 months|
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