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|Application / Reactivity||Human|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies|
|Immunofluorescence (IF)||27 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||35 Antibodies|
|Immunohistochemistry (IHC)||105 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||35 Antibodies|
|Immunoprecipitation (IP)||11 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Radioimmunoassay (RIA)||1 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Western Blotting (WB)||204 Antibodies|
|Antigen||Protein Kinase C, delta (PKCd) Antibodies|
|Conjugate||This PKC delta antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-PKC delta AntibodyTarget Details PKC delta Application Details Handling Images
|No Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
|Purification||Tissue culture supernatant|
|Immunogen||A synthetic peptide corresponding to residues near the C-terminus of human PKC delta was used as an immunogen.|
Target Details PKC deltaProduct Details anti-PKC delta Antibody Application Details Handling Images back to top
|Alternative Name||PRKCD (PKCd Antibody Abstract)|
PKC-delta has a biological activity which is closely related to Pkc1, and PKC-deltaactivates the Pkc1-mediated pathway through an activation of the Bck1 kinase.PKC-delta appears to play a critical role in growth control of yeast and mammalian cells.Suppression experiments suggest that PKC-delta desensitizes the pathway byregulating an aspect of G protein function (1). PKC-delta phosphorylates hRad9 in vitroand in cells exposed to genotoxic agents. It has been shown that PKC-delta is requiredfor binding of hRad9 to Bcl-2. In concert with these results, inhibition of PKC-deltaattenuates Rad9-mediated apoptosis. These findings demonstrate that PKC-delta isresponsible for the regulation of Rad9 in the Hus1-Rad1 complex and in the apoptoticresponse to DNA damage (2). It has been reported a mechanism for the regulation ofperipheral B-cell survival by serine/threonine protein kinase Cdelta (PKC-delta):spontaneous death of resting B cells is regulated by nuclear localization of PKC-deltathat contributes to phosphorylation of histone H2B at serine 14 (S14-H2B) (3). Alternate Names: anti-D14Ertd420e antibody, anti-MAY 1 antibody, anti-MAY1 antibody, anti-MGC49908antibody, anti-nPKC delta antibod, anti-PCKd antibody anti-PKC d antibody, anti-PKCdelta antibody, anti-PKCD antibody, anti-PKCdelta antibody, anti-PRKC D antibody,anti-PRKC delta antibody, anti-PRKCd antibody, anti-Protein Kinase C delta antibody,anti-Protein kinase C delta type antibody, anti-Protein Kinase Cdelta antibody.
Gene Symbol: PRKCD
|Gene ID||5580, 18753, 170538|
|Pathways||TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-PKC delta Antibody Target Details PKC delta Handling Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Flow Cytometry. In Western blot this antibody detects a band at approximately 78kDa.
Recommended dilutions: Flow Cytometry 1:15, Immunocytochemistry/Immunofluorescence 1:50-100, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:50, Western Blot 1:500
Protocol specific for PKC delta Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC delta Antibody Target Details PKC delta Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-PKC delta Antibody Target Details PKC delta Application Details Handling back to top