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anti-Human PKC delta antibody for Immunocytochemistry

Recommended PKC delta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, delta (PKCd) Antibodies
  • AI385711
  • D14Ertd420e
  • fb09d10
  • fv43b11
  • MAY1
  • may1
  • npkc-delta
  • nPKC-delta
  • Pkaca
  • PKC-delta
  • pkc-delta2
  • pkcd
  • Pkcd
  • PKCD
  • PKCdelta
  • PKC[d]
  • prkcd
  • PRKCD
  • tpa-1
  • wu:fb09d10
  • wu:fv43b11
  • zgc:56558
Epitope
C-Term
40
32
24
22
15
13
12
11
10
8
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
248
141
121
7
2
2
2
2
2
1
Host
Rabbit
258
19
Clonality
Monoclonal
Conjugate
This PKC delta antibody is un-conjugated
9
8
7
4
4
4
3
3
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
218
127
97
35
29
25
10
8
5
2
1
1
1
1
1
Supplier
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Catalog No. ABIN189679
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010155214 ABIN2884229 ICC ELISA Rabbit IgG AA 18-67 Log in to see Polyclonal
0.0010155214 ABIN2884231 ICC IHC ELISA WB Rabbit IgG pTyr52 Log in to see Polyclonal
0.0010155214 ABIN408071 ICC IF IHC (p) IHC WB Rabbit IgG Gly499 Log in to see Polyclonal
0.0010155214 ABIN1078462 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
0.0010155214 ABIN3201398 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see
0.0010155214 ABIN2985485 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal

Similar anti-PKC delta Antibodies

Application / Reactivity Human
ELISA 106 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 25 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 35 Antibodies
Immunohistochemistry (IHC) 98 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Western Blotting (WB) 196 Antibodies

General

Antigen Protein Kinase C, delta (PKCd) Antibodies
Epitope C-Term
(40), (32), (24), (22), (15), (13), (12), (11), (10), (8), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(248), (141), (121), (7), (2), (2), (2), (2), (2), (1)
Host Rabbit
(258), (19)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC delta antibody is un-conjugated
(9), (8), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(218), (127), (97), (35), (29), (25), (10), (8), (5), (2), (1), (1), (1), (1), (1)
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Product Details anti-PKC delta Antibody

Target Details PKC delta Application Details Handling Images
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Tissue culture supernatant
Immunogen A synthetic peptide corresponding to residues near the C-terminus of human PKC delta was used as an immunogen.
Clone EP1484Y
Isotype IgG

Target Details PKC delta

Product Details anti-PKC delta Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKCD (PKCd Antibody Abstract)
Background PKC-delta has a biological activity which is closely related to Pkc1, and PKC-deltaactivates the Pkc1-mediated pathway through an activation of the Bck1 kinase.PKC-delta appears to play a critical role in growth control of yeast and mammalian cells.Suppression experiments suggest that PKC-delta desensitizes the pathway byregulating an aspect of G protein function (1). PKC-delta phosphorylates hRad9 in vitroand in cells exposed to genotoxic agents. It has been shown that PKC-delta is requiredfor binding of hRad9 to Bcl-2. In concert with these results, inhibition of PKC-deltaattenuates Rad9-mediated apoptosis. These findings demonstrate that PKC-delta isresponsible for the regulation of Rad9 in the Hus1-Rad1 complex and in the apoptoticresponse to DNA damage (2). It has been reported a mechanism for the regulation ofperipheral B-cell survival by serine/threonine protein kinase Cdelta (PKC-delta):spontaneous death of resting B cells is regulated by nuclear localization of PKC-deltathat contributes to phosphorylation of histone H2B at serine 14 (S14-H2B) (3). Alternate Names: anti-D14Ertd420e antibody, anti-MAY 1 antibody, anti-MAY1 antibody, anti-MGC49908antibody, anti-nPKC delta antibod, anti-PCKd antibody anti-PKC d antibody, anti-PKCdelta antibody, anti-PKCD antibody, anti-PKCdelta antibody, anti-PRKC D antibody,anti-PRKC delta antibody, anti-PRKCd antibody, anti-Protein Kinase C delta antibody,anti-Protein kinase C delta type antibody, anti-Protein Kinase Cdelta antibody.
Gene Symbol: PRKCD
Gene ID 5580, 18753, 170538
Pathways TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PKC delta Antibody Target Details PKC delta Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Flow Cytometry. In Western blot this antibody detects a band at approximately 78kDa.
Recommended dilutions: Flow Cytometry 1:15, Immunocytochemistry/Immunofluorescence 1:50-100, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:50, Western Blot 1:500
Protocol Protocol specific for PKC delta Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-PKC delta Antibody Target Details PKC delta Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-PKC delta Antibody Target Details PKC delta Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PKC delta antibody (Protein Kinase C, delta) (C-Term) (ABIN189679) Western blot analysis on HeLa cell lysate using anti-PKC delta RabMAb (cat. # 110-573...