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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||55 Antibodies||106 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||7 Antibodies|
|Immunofluorescence (IF)||18 Antibodies||25 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||34 Antibodies|
|Immunohistochemistry (IHC)||42 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||16 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Western Blotting (WB)||101 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Antigen||Protein Kinase C, delta (PKCd) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This PKC delta antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-PKC delta AntibodyTarget Details PKC delta Application Details Handling References for anti-PKC delta Antibody (ABIN197068) Images
|Specificity||PKCδ antibody detects endogenous levels of PKC-delta only when phosphorylated at Serine 645.|
|Purification||Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.|
|Immunogen||The antiserum was produced against synthesized phosphopeptide derived from Human PKC-delta around the phosphorylation site of Serine 645 (R-L-Sp-Y-S).|
Target Details PKC deltaProduct Details anti-PKC delta Antibody Application Details Handling References for anti-PKC delta Antibody (ABIN197068) Images back to top
|Alternative Name||PRKCD (PKCd Antibody Abstract)|
|Background||Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.Synonyms: PKC delta, PKC-delta, PKCD, Protein kinase C delta type|
|Pathways||TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-PKC delta Antibody Target Details PKC delta Handling References for anti-PKC delta Antibody (ABIN197068) Images back to top
Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC delta Antibody Target Details PKC delta Application Details References for anti-PKC delta Antibody (ABIN197068) Images back to top
|Buffer||PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody (in aliquots) at -20 °C.|
References for anti-PKC delta Antibody (ABIN197068)Product Details anti-PKC delta Antibody Target Details PKC delta Application Details Handling Images back to top
Van Kolen, Slegers: "Atypical PKCzeta is involved in RhoA-dependent mitogenic signaling by the P2Y(12) receptor in C6 cells." in: The FEBS journal, Vol. 273, Issue 8, pp. 1843-54, 2006 (PubMed).
Villalba, Bi, Hu et al.: "Translocation of PKC[theta] in T cells is mediated by a nonconventional, PI3-K- and Vav-dependent pathway, but does not absolutely require phospholipase C." in: The Journal of cell biology, Vol. 157, Issue 2, pp. 253-63, 2002 (PubMed).
ImagesProduct Details anti-PKC delta Antibody Target Details PKC delta Application Details Handling References for anti-PKC delta Antibody (ABIN197068) back to top