|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||33 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||24 Antibodies|
|Immunohistochemistry (IHC)||106 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||28 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Radioimmunoassay (RIA)||1 Antibodies|
|Western Blotting (WB)||145 Antibodies|
|Antigen||Protein Kinase C, theta (PRKCQ) Antibodies|
|Conjugate||This PKC theta antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-PKC theta AntibodyTarget Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN359074) Images
|Specificity||This antibody reacts to PKC theta.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.|
Target Details PKC thetaProduct Details anti-PKC theta Antibody Application Details Handling References for anti-PKC theta Antibody (ABIN359074) Images back to top
|Alternative Name||PRKCQ / PRKCT (PRKCQ Antibody Abstract)|
|Background||Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. PKC theta is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.Synonyms: PKC theta, PKC-theta, Protein kinase C theta type, Protein kinase C theta type, nPKC-theta|
|Gene ID||5588, 9606|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-PKC theta Antibody Target Details PKC theta Handling References for anti-PKC theta Antibody (ABIN359074) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC theta Antibody Target Details PKC theta Application Details References for anti-PKC theta Antibody (ABIN359074) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PKC theta Antibody (ABIN359074)Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling Images back to top
Carrasco, Merida: "Diacylglycerol-dependent binding recruits PKCtheta and RasGRP1 C1 domains to specific subcellular localizations in living T lymphocytes." in: Molecular biology of the cell, Vol. 15, Issue 6, pp. 2932-42, 2004 (PubMed).
Burchfield, Lennard, Narasimhan et al.: "Akt mediates insulin-stimulated phosphorylation of Ndrg2: evidence for cross-talk with protein kinase C theta." in: The Journal of biological chemistry, Vol. 279, Issue 18, pp. 18623-32, 2004 (PubMed).
Sun, Mao, Rao et al.: "Association of CBFA2 mutation with decreased platelet PKC-theta and impaired receptor-mediated activation of GPIIb-IIIa and pleckstrin phosphorylation: proteins regulated by CBFA2 play a role in ..." in: Blood, Vol. 103, Issue 3, pp. 948-54, 2004 (PubMed).
Ishaq, DeGray, Natarajan: "Protein kinase C theta modulates nuclear receptor-corepressor interaction during T cell activation." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39296-302, 2003 (PubMed).
Cipriani, Knowles, Chen et al.: "Involvement of classical and novel protein kinase C isoforms in the response of human V gamma 9V delta 2 T cells to phosphate antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 10, pp. 5761-70, 2002 (PubMed).
ImagesProduct Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN359074) back to top