anti-Human PKC theta antibody for Enzyme Immunoassay

Recommended PKC theta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, theta (PRKCQ) Antibodies
  • PRKCT
  • nPKC-theta
  • A130035A12Rik
  • AW494342
  • PKC-0
  • PKC-theta
  • PKCtheta
  • Pkcq
  • protein kinase C, theta
  • protein kinase C theta
  • PRKCQ
  • PKC
  • Prkcq
Epitope
C-Term
47
44
38
17
11
10
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
237
139
133
12
10
10
7
4
3
2
1
1
Host
Rabbit
223
30
3
1
Clonality
Polyclonal
Conjugate
This PKC theta antibody is un-conjugated
6
6
6
6
6
5
5
5
3
3
3
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
170
115
72
48
40
38
23
7
6
3
3
2
2
2
1
1
1
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Catalog No. ABIN359074
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5611777 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

General

Antigen Protein Kinase C, theta (PRKCQ) Antibodies
Epitope C-Term
(47), (44), (38), (17), (11), (10), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(237), (139), (133), (12), (10), (10), (7), (4), (3), (2), (1), (1)
Host Rabbit
(223), (30), (3), (1)
Clonality
Polyclonal
Conjugate This PKC theta antibody is un-conjugated
(6), (6), (6), (6), (6), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(170), (115), (72), (48), (40), (38), (23), (7), (6), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-PKC theta Antibody

Target Details PKC theta Application Details Handling References for anti-PKC theta antibody (ABIN359074) Images
Specificity This antibody reacts to PKC theta.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.
Isotype Ig

Target Details PKC theta

Product Details anti-PKC theta Antibody Application Details Handling References for anti-PKC theta antibody (ABIN359074) Images back to top
Antigen
Alternative Name PRKCQ / PRKCT (PRKCQ Antibody Abstract)
Background Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. PKC theta is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.Synonyms: PKC theta, PKC-theta, Protein kinase C theta type, Protein kinase C theta type, nPKC-theta
Gene ID 5588, 9606
UniProt Q04759
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-PKC theta Antibody Target Details PKC theta Handling References for anti-PKC theta antibody (ABIN359074) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details References for anti-PKC theta antibody (ABIN359074) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PKC theta antibody (ABIN359074)

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling Images back to top
Background publications

Carrasco, Merida: "Diacylglycerol-dependent binding recruits PKCtheta and RasGRP1 C1 domains to specific subcellular localizations in living T lymphocytes." in: Molecular biology of the cell, Vol. 15, Issue 6, pp. 2932-42, 2004

Sun, Mao, Rao et al.: "Association of CBFA2 mutation with decreased platelet PKC-theta and impaired receptor-mediated activation of GPIIb-IIIa and pleckstrin phosphorylation: proteins regulated by CBFA2 play a role in ..." in: Blood, Vol. 103, Issue 3, pp. 948-54, 2004

Burchfield, Lennard, Narasimhan, Hughes, Wasinger, Corthals, Okuda, Kondoh, Biden, Schmitz-Peiffer: "Akt mediates insulin-stimulated phosphorylation of Ndrg2: evidence for cross-talk with protein kinase C theta." in: The Journal of biological chemistry, Vol. 279, Issue 18, pp. 18623-32, 2004

Ishaq, DeGray, Natarajan: "Protein kinase C theta modulates nuclear receptor-corepressor interaction during T cell activation." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39296-302, 2003

Cipriani, Knowles, Chen, Battistini, Brosnan: "Involvement of classical and novel protein kinase C isoforms in the response of human V gamma 9V delta 2 T cells to phosphate antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 10, pp. 5761-70, 2002

Images

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling References for anti-PKC theta antibody (ABIN359074) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Protein Kinase C, theta (PRKCQ) (C-Term) antibody (ABIN359074) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-Protein Kinase C, theta (PRKCQ) (C-Term) antibody (ABIN359074) Western blot analysis of anti-PKC theta Pab in placenta lysate. PKC theta (Arrow) was...