|Application / Reactivity||Rat (Rattus)||Mouse (Murine)||Human|
|ELISA||8 Antibodies||16 Antibodies||70 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies||8 Antibodies|
|Immunofluorescence (IF)||8 Antibodies||6 Antibodies|
|Immunohistochemistry (IHC)||5 Antibodies||17 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||22 Antibodies||35 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies|
|Antigen||Receptor-Interacting Serine-threonine Kinase 2 (RIPK2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This RIPK2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-RIPK2 AntibodyTarget Details RIPK2 Application Details Handling References for anti-RIPK2 Antibody (ABIN500599) Images
|Specificity||This antibody detects RICK at N-term.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, mouse, rat|
|Purification||Peptide affinity chromatography|
|Immunogen||RICK antibody was raised against a peptide corresponding to amino acids near the amino terminus of human RICK.|
Target Details RIPK2Product Details anti-RIPK2 Antibody Application Details Handling References for anti-RIPK2 Antibody (ABIN500599) Images back to top
|Alternative Name||RIPK2 (RIPK2 Antibody Abstract)|
|Background||Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively, (1-3). RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-kappaB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1b. The messenger RNA of RICK is expressed in multiple human tissues.Synonyms: CARD-containing IL-1 beta ICE-kinase, CARDIAK, RICK, RIP-2, RIP-like-interacting CLARP kinase, RIP2, Receptor-interacting protein 2, Receptor-interacting serine/threonine-protein kinase 2|
|Research Area||Apoptosis/Necrosis, Signaling|
|Pathways||TCR Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-RIPK2 Antibody Target Details RIPK2 Handling References for anti-RIPK2 Antibody (ABIN500599) Images back to top
ELISA. Western blot: 1: 1000, an approximately 60 kDa band can be detected. Immuoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RIPK2 Antibody Target Details RIPK2 Application Details References for anti-RIPK2 Antibody (ABIN500599) Images back to top
|Buffer||PBS containing 0.02 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|
References for anti-RIPK2 Antibody (ABIN500599)Product Details anti-RIPK2 Antibody Target Details RIPK2 Application Details Handling Images back to top
McCarthy, Ni, Dixit: "RIP2 is a novel NF-kappaB-activating and cell death-inducing kinase." in: The Journal of biological chemistry, Vol. 273, Issue 27, pp. 16968-75, 1998 (PubMed).
Inohara, del Peso, Koseki et al.: "RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12296-300, 1998 (PubMed).
ImagesProduct Details anti-RIPK2 Antibody Target Details RIPK2 Application Details Handling References for anti-RIPK2 Antibody (ABIN500599) back to top