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anti-Human SH2B3 antibody for ELISA

Recommended SH2B3 Antibody (supplied by: Log in to see )

Antigen
SH2B Adaptor Protein 3 (SH2B3) Antibodies
  • AI429800
  • IDDM20
  • Lnk
  • LNK
Epitope
N-Term
17
5
3
1
1
1
Reactivity
Human
62
18
17
2
Host
Goat
27
19
17
Clonality
Polyclonal
Conjugate
This SH2B3 antibody is un-conjugated
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
46
17
16
13
11
6
5
3
2
1
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Catalog No. ABIN185272
$ 318.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012092679 ABIN2465907 ELISA WB Goat N-Term Log in to see Polyclonal
0.0012092679 ABIN292511 ELISA WB Goat N-Term Log in to see Polyclonal
0.0012092679 ABIN1859674 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 335-561 Log in to see Polyclonal
0.0012092679 ABIN1104261 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0012092679 ABIN2302740 ELISA WB Biotin Goat IgG N-Term Log in to see Polyclonal
0.0012092679 ABIN2302742 ELISA WB FITC Goat IgG N-Term Log in to see Polyclonal
0.0012092679 ABIN2302745 ELISA WB PE Goat IgG N-Term Log in to see Polyclonal
0.0012092679 ABIN2302755 ELISA WB Alkaline Phosphatase (AP) Goat IgG N-Term Log in to see Polyclonal
0.0012092679 ABIN2302757 ELISA WB APC Goat IgG N-Term Log in to see Polyclonal
0.0012092679 ABIN151695 ELISA WB Goat N-Term Log in to see Polyclonal
0.0012092679 ABIN2302753 ELISA WB Goat IgG N-Term Log in to see Polyclonal
0.0012092679 ABIN2571066 ELISA WB Biotin Rabbit IgG AA 335-561 Log in to see Polyclonal
0.0012092679 ABIN2302743 ELISA WB HRP Goat IgG N-Term Log in to see Polyclonal
0.0012092679 ABIN2571065 ELISA Rabbit IgG AA 335-561 Log in to see Polyclonal
0.0012092679 ABIN2563705 ELISA WB Goat IgG N-Term Log in to see Polyclonal
0.0012092679 ABIN2302759 ELISA WB Goat N-Term Log in to see Polyclonal
0.0012092679 ABIN809148 IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-SH2B3 antibody for ELISA

Similar anti-SH2B3 Antibodies

Application / Reactivity Human
ELISA 18 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Western Blotting (WB) 48 Antibodies

General

Antigen SH2B Adaptor Protein 3 (SH2B3) Antibodies
Epitope N-Term
(17), (5), (3), (1), (1), (1)
Reactivity Human
(62), (18), (17), (2)
Host Goat
(27), (19), (17)
Clonality
Polyclonal
Conjugate This SH2B3 antibody is un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application ELISA, Western Blotting (WB)
(46), (17), (16), (13), (11), (6), (5), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-SH2B3 Antibody

Target Details SH2B3 Application Details Handling References for anti-SH2B3 Antibody (ABIN185272) Images
Blocking Peptide Blocking peptide for this product available: ABIN370259
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence NGPALQPSSPC, from the N Terminus of the protein sequence according to NP_005466.1.

Target Details SH2B3

Product Details anti-SH2B3 Antibody Application Details Handling References for anti-SH2B3 Antibody (ABIN185272) Images back to top
Antigen
Alternative Name LNK / SH2B3 (SH2B3 Antibody Abstract)
Background Synonyms: SH2B3, LNK, SH2B adaptor protein 3, lymphocyte adaptor protein
Gene ID 10019
NCBI Accession NP_005466
Pathways TCR Signaling

Application Details

Product Details anti-SH2B3 Antibody Target Details SH2B3 Handling References for anti-SH2B3 Antibody (ABIN185272) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 70 kDa band observed in lysates of cell line MOLT4 (calculated MW of 63.2 kDa according to NP_005466.1). In transfected HEK293 transiently expressing SH2B3 a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1.0 µg/mL.
Restrictions For Research Use only

Handling

Product Details anti-SH2B3 Antibody Target Details SH2B3 Application Details References for anti-SH2B3 Antibody (ABIN185272) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-SH2B3 Antibody (ABIN185272)

Product Details anti-SH2B3 Antibody Target Details SH2B3 Application Details Handling Images back to top
Background publications

Li, He, Schembri-King, Jakes, Hayashi: "Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 10, pp. 5199-206, 2000

Images

Product Details anti-SH2B3 Antibody Target Details SH2B3 Application Details Handling References for anti-SH2B3 Antibody (ABIN185272) back to top
Supplier Images
 image for anti-SH2B3 antibody (SH2B Adaptor Protein 3) (N-Term) (ABIN185272) ABIN185272 (0.3µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Primary...