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|Application / Reactivity||Human|
|Agonist (Agon)||2 Antibodies|
|Blocking Antibody (Inhibition)||1 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||11 Antibodies|
|Flow Cytometry (FACS)||38 Antibodies|
|Functional Studies (Func)||4 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||12 Antibodies|
|Immunofluorescence (IF)||28 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||81 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||2 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||28 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Antigen||Transforming Growth Factor, beta 1 (TGFB1) Antibodies|
|Conjugate||This TGFB1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-TGFB1 AntibodyTarget Details TGFB1 Application Details Handling References for anti-TGFB1 Antibody (ABIN181152) Images
|Specificity||This antibody detects TGF-3.|
|Characteristics||Synonyms: Transforming growth factor beta-1, TGFB, LAP, Latency-associated peptide|
|Purification||Affinity Chromatography on Protein G|
|Immunogen||Highly pure (>98%) recombinant Human TGFB1|
Target Details TGFB1Product Details anti-TGFB1 Antibody Application Details Handling References for anti-TGFB1 Antibody (ABIN181152) Images back to top
|Alternative Name||TGFB1 (TGFB1 Antibody Abstract)|
|Background||Synonyms: TGF-beta-1, TGFB, Transforming growth factor beta-1|
|Pathways||TCR Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway|
Application DetailsProduct Details anti-TGFB1 Antibody Target Details TGFB1 Handling References for anti-TGFB1 Antibody (ABIN181152) Images back to top
ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 100 pg/mL of recombinant Human TGFB1 when used inconjunction with compatible secondary reagents. Western Blot: To detect Human TGFB1 by Western Blot analysis this antibody can be usedat a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human TGFB1 is 2.0-4.0 ng/lane, undernon-reducing conditions. In a dot blot, a 1/500 dilution of this antibody exhibited complete cross reactivity withHuman TGFB2 and TGFB3 isotypes. Reduced cross reactivity was shown at a 1/1000dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TGFB1 Antibody Target Details TGFB1 Application Details References for anti-TGFB1 Antibody (ABIN181152) Images back to top
|Reconstitution||Restore in sterile water to a concentration of 1.0 mg/mL.|
|Buffer||PBS without preservatives|
|Storage||4 °C/-20 °C|
The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-TGFB1 Antibody (ABIN181152)Product Details anti-TGFB1 Antibody Target Details TGFB1 Application Details Handling Images back to top
Sato, Ozaki, Oh, Meguro, Hatanaka, Nagai, Muroi, Ozawa: "Nitric oxide plays a critical role in suppression of T-cell proliferation by mesenchymal stem cells." in: Blood, Vol. 109, Issue 1, pp. 228-34, 2006
ImagesProduct Details anti-TGFB1 Antibody Target Details TGFB1 Application Details Handling References for anti-TGFB1 Antibody (ABIN181152) back to top