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|Antigen||Transforming Growth Factor, beta 1 (TGFB1) Antibodies|
|Conjugate||This TGFB1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|1 reference available|
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Product Details anti-TGFB1 AntibodyTarget Details TGFB1 Application Details Handling ProductDetails: References for anti-TGFB1 antibody (ABIN181152) Images
|Specificity||This antibody detects TGF-3.|
|Characteristics||Synonyms: Transforming growth factor beta-1, TGFB, LAP, Latency-associated peptide|
|Purification||Affinity Chromatography on Protein G|
|Immunogen||Highly pure (>98%) recombinant Human TGFB1|
Target Details TGFB1Product Details anti-TGFB1 Antibody Application Details Handling ProductDetails: References for anti-TGFB1 antibody (ABIN181152) Images back to top
|Alternative Name||TGFB1 (TGFB1 Antibody Abstract)|
|Background||Synonyms: TGF-beta-1, TGFB, Transforming growth factor beta-1|
|Pathways||EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy|
Application DetailsProduct Details anti-TGFB1 Antibody Target Details TGFB1 Handling ProductDetails: References for anti-TGFB1 antibody (ABIN181152) Images back to top
ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 100 pg/mL of recombinant Human TGFB1 when used inconjunction with compatible secondary reagents. Western Blot: To detect Human TGFB1 by Western Blot analysis this antibody can be usedat a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human TGFB1 is 2.0-4.0 ng/lane, undernon-reducing conditions. In a dot blot, a 1/500 dilution of this antibody exhibited complete cross reactivity withHuman TGFB2 and TGFB3 isotypes. Reduced cross reactivity was shown at a 1/1000dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TGFB1 Antibody Target Details TGFB1 Application Details ProductDetails: References for anti-TGFB1 antibody (ABIN181152) Images back to top
|Reconstitution||Restore in sterile water to a concentration of 1.0 mg/mL.|
|Buffer||PBS without preservatives|
|Storage||4 °C/-20 °C|
The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ProductDetails: References for anti-TGFB1 antibody (ABIN181152)Product Details anti-TGFB1 Antibody Target Details TGFB1 Application Details Handling Images back to top
Sato, Ozaki, Oh, Meguro, Hatanaka, Nagai, Muroi, Ozawa: "Nitric oxide plays a critical role in suppression of T-cell proliferation by mesenchymal stem cells." in: Blood, Vol. 109, Issue 1, pp. 228-34, 2006
ImagesProduct Details anti-TGFB1 Antibody Target Details TGFB1 Application Details Handling ProductDetails: References for anti-TGFB1 antibody (ABIN181152) back to top