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anti-Mouse (Murine) TGFB1 antibody for Flow Cytometry

Recommended TGFB1 Antibody (supplied by: Log in to see )

Antigen
Transforming Growth Factor, beta 1 (TGFB1) Antibodies
  • ai39657
  • betaig-h3
  • bigh3
  • cdb1
  • cdg2
  • cdgg1
  • CED
  • ced
  • csd
  • csd1
  • csd2
  • csd3
  • DPD1
  • dpd1
  • ebmd
  • LAP
  • lap
  • lcd1
  • MGC146225
  • tgf
  • TGF-beta
  • tgf-beta
  • TGF-BETA-1
  • TGF-beta1
  • TGF-beta5
  • TGFB
  • Tgfb
  • tgfb
  • Tgfb-1
  • tgfb1
  • TGFB1
  • TGFB4
  • tgfb5
  • TGFbeta
  • tgfbeta
  • TGFbeta1
  • TGFBI
  • wu:fb13a07
  • xx:ai39657
Reactivity
Human, Mouse (Murine)
254
74
63
24
13
13
11
10
7
6
4
3
3
3
3
1
1
Host
Mouse
230
101
4
3
3
2
1
Clonality
Monoclonal
Conjugate
This TGFB1 antibody is conjugated to Biotin
31
23
11
9
8
3
3
3
3
3
3
3
3
3
3
1
1
1
Application
Flow Cytometry (FACS)
201
190
98
40
38
33
31
26
18
12
11
4
3
2
2
2
2
2
1
1
Supplier
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Catalog No. ABIN2661297
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010468885 ABIN2665942 Agon FACS Mouse IgG1 kappa Log in to see 19D8 13
0.0010468885 ABIN2665941 Agon FACS Mouse IgG1 kappa Log in to see 19D8 13

Top referenced anti-TGFB1 antibody for Flow Cytometry

Similar anti-TGFB1 Antibodies

Application / Reactivity Human Mouse (Murine)
Western Blotting (WB) 157 Antibodies 47 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Neutralization (Neut) 12 Antibodies
Immunoprecipitation (IP) 3 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies 9 Antibodies
Immunohistochemistry (IHC) 81 Antibodies 34 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 11 Antibodies
Immunofluorescence (IF) 28 Antibodies 11 Antibodies
Immunocytochemistry (ICC) 12 Antibodies

General

Antigen Transforming Growth Factor, beta 1 (TGFB1) Antibodies
Reactivity Human, Mouse (Murine)
(254), (74), (63), (24), (13), (13), (11), (10), (7), (6), (4), (3), (3), (3), (3), (1), (1)
Host Mouse
(230), (101), (4), (3), (3), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TGFB1 antibody is conjugated to Biotin
(31), (23), (11), (9), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application Flow Cytometry (FACS)
(201), (190), (98), (40), (38), (33), (31), (26), (18), (12), (11), (4), (3), (2), (2), (2), (2), (2), (1), (1)
Pubmed 13 references available
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Product Details anti-TGFB1 Antibody

Target Details TGFB1 Application Details Handling References for anti-TGFB1 Antibody (ABIN2661297) Images
Purification The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Clone 19D8
Isotype IgG1 kappa

Target Details TGFB1

Product Details anti-TGFB1 Antibody Application Details Handling References for anti-TGFB1 Antibody (ABIN2661297) Images back to top
Antigen
Alternative Name TGF-Beta1 (TGFB1 Antibody Abstract)
Background TGF-β1 is a multifunctional cytokine that plays pivotal roles in diverse biological processes. TGF-β1 is synthesized as a 390 amino acid precursor that is cleaved by furin, localized in the trans-Golgi network, in residue 278. Furin processes the TGF-β1 precursor at the carboxyl side of the consensus sequence RHRR which precedes the NH2-terminal Ala 279 residue of the mature TGF-β1. The TGF-β1 precursor includes the latency-associated peptide (LAP dimer) in the N-terminal portion and the 25 kD portion that constitutes the mature TGF-β1 in the C-terminal. LAP dimer and the TGF-β1 mature protein remain non-covalently associated after furin cleavage and this complex does not bind to the TFG-β1 receptor. In addition, the TGF-β1 latent complex is joined covalently through LAP to LTBP. The TGF-β1 active form requires dissociation from LAP. Some activators can release TGF-β1 from LAP such as thrombospondin-1, reactive oxygen species, and the integrins avb6 and avb8. Mouse TGF-β1 converts naive T cells into regulatory T (Treg) cells that prevent autoimmunity. Although human TGF- β1 is widely used for inducing FOXP3+ in vitro, it might not be an essential factor for human Treg differentiation. Th17 murine can be induced from naive CD4+ T cells by the combination of TGF-β1 and IL-6 or IL-21. Nevertheless, the regulation of human Th17 differentiation is distinct. TGF-β1 seems to have dual effects on human Th17 differentiation in a dose-dependent manner. While TGF-β1 is required for the expression of RORgt, in human naive CD4+ T cells from cord blood, TGF-β1 can inhibit the function of RORgt at high doses. By using serum-free medium, it has been clarified that the optimum conditions for human Th17 differentiation are TGF-β1, IL-1b, and IL-2 in combination with IL-6, IL-21, or IL-23.
Pathways TCR Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-TGFB1 Antibody Target Details TGFB1 Handling References for anti-TGFB1 Antibody (ABIN2661297) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-TGFB1 Antibody Target Details TGFB1 Application Details References for anti-TGFB1 Antibody (ABIN2661297) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-TGFB1 Antibody (ABIN2661297)

Product Details anti-TGFB1 Antibody Target Details TGFB1 Application Details Handling Images back to top
Background publications

Oida, Weiner: "Murine CD4 T cells produce a new form of TGF-β as measured by a newly developed TGF-β bioassay." in: PLoS ONE, Vol. 6, Issue 4, pp. e18365, 2011

Takatori, Kanno, Chen, OShea: "New complexities in helper T cell fate determination and the implications for autoimmune diseases." in: Modern rheumatology / the Japan Rheumatism Association, Vol. 18, Issue 6, pp. 533-41, 2008

Manel, Unutmaz, Littman: "The differentiation of human T(H)-17 cells requires transforming growth factor-beta and induction of the nuclear receptor RORgammat." in: Nature immunology, Vol. 9, Issue 6, pp. 641-9, 2008

Veldhoen, Uyttenhove, van Snick, Helmby, Westendorf, Buer, Martin, Wilhelm, Stockinger: "Transforming growth factor-beta 'reprograms' the differentiation of T helper 2 cells and promotes an interleukin 9-producing subset." in: Nature immunology, Vol. 9, Issue 12, pp. 1341-6, 2008

Acosta-Rodriguez, Napolitani, Lanzavecchia, Sallusto: "Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells." in: Nature immunology, Vol. 8, Issue 9, pp. 942-9, 2007

Mucida, Park, Kim, Turovskaya, Scott, Kronenberg, Cheroutre: "Reciprocal TH17 and regulatory T cell differentiation mediated by retinoic acid." in: Science (New York, N.Y.), Vol. 317, Issue 5835, pp. 256-60, 2007

Puthawala, Hadjiangelis, Jacoby, Bayongan, Zhao, Yang, Devitt, Horan, Weinreb, Lukashev, Violette, Grant, Colarossi, Formenti, Munger: "Inhibition of integrin alpha(v)beta6, an activator of latent transforming growth factor-beta, prevents radiation-induced lung fibrosis." in: American journal of respiratory and critical care medicine, Vol. 177, Issue 1, pp. 82-90, 2007

Park: "Fine tuning and cross-talking of TGF-beta signal by inhibitory Smads." in: Journal of biochemistry and molecular biology, Vol. 38, Issue 1, pp. 9-16, 2005

Valcourt, Kowanetz, Niimi, Heldin, Moustakas: "TGF-beta and the Smad signaling pathway support transcriptomic reprogramming during epithelial-mesenchymal cell transition." in: Molecular biology of the cell, Vol. 16, Issue 4, pp. 1987-2002, 2005

Zou, Sun: "Overexpression of human transforming growth factor-beta1 using a recombinant CHO cell expression system." in: Protein expression and purification, Vol. 37, Issue 2, pp. 265-72, 2004

Annes, Munger, Rifkin: "Making sense of latent TGFbeta activation." in: Journal of cell science, Vol. 116, Issue Pt 2, pp. 217-24, 2002

Dennler, Itoh, Vivien, ten Dijke, Huet, Gauthier: "Direct binding of Smad3 and Smad4 to critical TGF beta-inducible elements in the promoter of human plasminogen activator inhibitor-type 1 gene." in: The EMBO journal, Vol. 17, Issue 11, pp. 3091-100, 1998

Dubois, Laprise, Blanchette, Gentry, Leduc: "Processing of transforming growth factor beta 1 precursor by human furin convertase." in: The Journal of biological chemistry, Vol. 270, Issue 18, pp. 10618-24, 1995

Images

Product Details anti-TGFB1 Antibody Target Details TGFB1 Application Details Handling References for anti-TGFB1 Antibody (ABIN2661297) back to top
Supplier Images
ELISA image for anti-TGFB1 antibody (Transforming Growth Factor, beta 1)  (Biotin) (ABIN2661297) anti-Transforming Growth Factor, beta 1 (TGFB1) antibody (Biotin)