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|Application / Reactivity||Human||Rat (Rattus)||Mouse (Murine)|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies|
|Western Blotting (WB)||124 Antibodies||58 Antibodies||58 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||12 Antibodies||9 Antibodies||7 Antibodies|
|Immunohistochemistry (IHC)||26 Antibodies||17 Antibodies||14 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||14 Antibodies||14 Antibodies||14 Antibodies|
|Antigen||Vav 1 Oncogene (VAV1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This VAV1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-VAV1 AntibodyTarget Details VAV1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of VAV1 protein. (region surrounding Ala168)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity Chromatography using epitope-specific immunogen.|
Target Details VAV1Product Details anti-VAV1 Antibody Application Details Handling Images back to top
|Alternative Name||VAV1 (VAV1 Antibody Abstract)|
|Background||The Vav gene was originally identified on the basis of its oncogenic activation during the course of gene transfer assays. The major translational product of the Vav proto-oncogene has been identified as a protein containing an array of structural motifs. Contained within its amino terminus are a helix-loop-helix domain and a leucine zipper motif similar to that of Myc family proteins, deletion of this region of p95Vav causes its oncogenic activation. In addition, p95Vav contains an SH2 domain, which could indicate its role as a substrate for tyrosine kinases. Expression of p95Vav is limited exclusively to cells of hematopoietic origin, including those of the erythroid, lymphoid and myeloid lineages. These results suggest that p95Vav may represent a new type of signal transduction molecule involved in the transduction of tyrosine phosphorylation signaling into transcriptional events.Synonyms: Proto-oncogene vav, VAV-1|
|Molecular Weight||approx. 100 kDa|
|Research Area||Cancer, Transcription Factors|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-VAV1 Antibody Target Details VAV1 Handling Images back to top
ELISA: 1/5000approx. 1/10000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-VAV1 Antibody Target Details VAV1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-VAV1 Antibody Target Details VAV1 Application Details Handling back to top