anti-Fish Vimentin antibody for Western Blotting

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Amphibian, Fish
929
406
356
221
138
132
121
83
81
52
51
43
41
37
30
12
8
7
5
5
3
3
2
2
2
1
1
1
1
1
Host
Mouse
569
321
30
17
12
9
9
6
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
29
27
26
23
18
16
13
13
13
13
13
13
13
13
12
11
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
739
345
328
305
255
252
250
143
87
40
15
14
13
10
8
7
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN126716
$ 335.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1824144 IHC WB Mouse IgG1 Log in to see XL-VIM-14-13
1 ABIN930870 IHC WB Mouse IgG1 Log in to see XL-VIM-14-13

General

Antigen Vimentin (VIM) Antibodies
Reactivity Amphibian, Fish
(929), (406), (356), (221), (138), (132), (121), (83), (81), (52), (51), (43), (41), (37), (30), (12), (8), (7), (5), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(569), (321), (30), (17), (12), (9), (9), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(29), (27), (26), (23), (18), (16), (13), (13), (13), (13), (13), (13), (13), (13), (12), (11), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(739), (345), (328), (305), (255), (252), (250), (143), (87), (40), (15), (14), (13), (10), (8), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN126716) Images
Purification Supernatant
Immunogen Vimentin from cytoskeletal fraction of XLKE cells (cultured Xenopus laevis kidney epithelial cells).
Clone XL-VIM-14-13
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN126716) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is an intermediate filament protein which is present in all cells of mesenchymal origin. Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.Synonyms: VIM
Gene ID 7962
UniProt Q92155
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN126716) Images back to top
Application Notes Immunohistochemistry on Frozen Sections. Western blotting. Immunoelectron Microscopy. Dilution buffer: 0.15 M PBS with 0.1 % BSA and 0.09 % Sodium Azide. Working Diutions: Ready-to-use for Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN126716) Images back to top
Format Liquid
Buffer 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-Vimentin antibody (ABIN126716)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Rungger-Bra: "Retinal patterning by Pax6-dependent cell adhesion molecules." in: Developmental neurobiology, Vol. 70, Issue 11, pp. 764-80, 2010

Zirwes, Kouzmenko, Peters, Franke, Schmidt-Zachmann: "Topogenesis of a nucleolar protein: determination of molecular segments directing nucleolar association." in: Molecular biology of the cell, Vol. 8, Issue 2, pp. 231-48, 1997

Herrmann, Munick, Brettel, Fouquet, Markl: "Vimentin in a cold-water fish, the rainbow trout: highly conserved primary structure but unique assembly properties." in: Journal of cell science, Vol. 109 ( Pt 3), pp. 569-78, 1997

Herrmann, Eckelt, Brettel, Grund, Franke: "Temperature-sensitive intermediate filament assembly. Alternative structures of Xenopus laevis vimentin in vitro and in vivo." in: Journal of molecular biology, Vol. 234, Issue 1, pp. 99-113, 1993