anti-Human Vimentin antibody for ELISA

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Epitope
AA 31-80, pSer56
40
25
24
22
21
18
15
14
14
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
928
406
356
221
138
132
121
83
81
52
51
43
41
37
30
12
8
7
5
5
4
3
3
2
2
1
1
1
1
1
Host
Rabbit
570
320
30
17
12
9
9
6
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
29
27
26
23
18
16
13
13
13
13
13
13
13
13
12
11
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
739
345
327
305
254
252
250
144
87
40
15
14
13
10
8
7
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN1531760
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1532790 IHC ELISA WB Rabbit IgG AA 31-80 Log in to see Polyclonal 2
1 ABIN1533459 IHC ELISA WB Rabbit IgG AA 411-460 Log in to see Polyclonal 1
1 ABIN1531759 IHC ELISA WB Rabbit IgG AA 56-105, pSer83 Log in to see Polyclonal
1 ABIN1532789 ELISA WB Rabbit IgG AA 56-105 Log in to see Polyclonal
1 ABIN969454 IHC ELISA WB Mouse IgG1 AA 2-466 Log in to see 4F2E9 2
1 ABIN2869381 ELISA IHC WB Mouse IgG1 AA 2-466 Log in to see 4F2E9 2
1 ABIN969453 IHC ELISA WB Mouse IgG1 Log in to see 9E7E7 4
1 ABIN2869230 ELISA IHC WB Mouse IgG1 Log in to see 9E7E7-5G3F10-5G3F10E5 1
1 ABIN192298 ELISA FACS ICC WB PE Mouse IgG1 Log in to see VI-RE-1 1
1 ABIN1558082 ICC FACS ELISA WB APC Mouse IgG1 Log in to see VI-RE-1 1
1 ABIN94494 ICC FACS ELISA WB Mouse IgG1 Log in to see VI-RE-1 1
1 ABIN3182648 ELISA IHC WB Rabbit IgG pSer56 Log in to see Polyclonal
1 ABIN3187473 ELISA IF IHC WB Rabbit IgG Tyr319 Log in to see Polyclonal
1 ABIN3187472 ELISA IF IHC WB Rabbit IgG Tyr317 Log in to see Polyclonal
1 ABIN3187474 ELISA WB Rabbit IgG Tyr321 Log in to see Polyclonal
1 ABIN3182647 ELISA IHC WB Rabbit IgG pSer83 Log in to see Polyclonal
1 ABIN1574031 IHC ELISA WB Rabbit IgG AA 300-350 Log in to see Polyclonal 4
1 ABIN1452456 ELISA WB Rabbit IgG pTyr38 Log in to see Polyclonal
1 ABIN3187471 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN269144 CyTOF ELISA FACS ICC IF WB Mouse IgG1 Log in to see VI-RE-1

General

Antigen Vimentin (VIM) Antibodies
Epitope AA 31-80, pSer56
(40), (25), (24), (22), (21), (18), (15), (14), (14), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(928), (406), (356), (221), (138), (132), (121), (83), (81), (52), (51), (43), (41), (37), (30), (12), (8), (7), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(570), (320), (30), (17), (12), (9), (9), (6)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(29), (27), (26), (23), (18), (16), (13), (13), (13), (13), (13), (13), (13), (13), (12), (11), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(739), (345), (327), (305), (254), (252), (250), (144), (87), (40), (15), (14), (13), (10), (8), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN1531760) Images
Specificity

Vimentin (Phospho-Ser56) Antibody detects endogenous levels of Vimentin only when phosphorylated at Ser56.

PhosphorylationH:S56

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Vimentin around the phosphorylation site of Ser56.
Isotype IgG

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN1531760) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background

Synonyms: VIME, vimentin

NCBI Gene Symbol: VIM

Molecular Weight 53 kDa
Gene ID 7431
OMIM 193060
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN1531760) Images back to top
Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Comment

Unigene-Number: Hs.642813 (NCBI Gene Symbol: VIM)

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN1531760) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-Vimentin antibody (ABIN1531760)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Rasmussen, Ji, Eddes, Moritz, Reid, Simpson, Dorow: "Two-dimensional electrophoretic analysis of human breast carcinoma proteins: mapping of proteins that bind to the SH3 domain of mixed lineage kinase MLK2." in: Electrophoresis, Vol. 18, Issue 3-4, pp. 588-98, 1997

Gupta, Aubin, Waye: "Isolation of a human vimentin cDNA with a long 3'-noncoding region from a human osteosarcoma cell line (MG-63)." in: Gene, Vol. 86, Issue 2, pp. 303-4, 1990

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN1531760) back to top
Supplier Images
Western Blotting (WB) image for anti-Vimentin (VIM) (pSer56), (AA 31-80) antibody (ABIN1531760) Western blot analysis of extracts from A549 cells treated with Nocodazole 1ug/ml 16h,...
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (pSer56), (AA 31-80) antibody (ABIN1531760) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vime...