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anti-Human Vimentin antibody for ELISA

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Epitope
pSer56
23
17
16
16
16
15
14
12
12
8
7
7
4
4
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
806
294
254
205
118
110
95
77
75
42
39
32
30
29
25
7
5
4
3
2
2
1
1
1
1
1
1
Host
Rabbit
519
277
30
15
12
7
5
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
27
26
23
18
14
13
12
11
11
11
11
11
11
11
11
9
5
5
5
4
3
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
671
299
290
290
239
233
232
128
104
25
15
12
8
8
5
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN1531760
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012584153 ABIN1532790 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.0012584153 ABIN1533459 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0012584153 ABIN969454 IHC ELISA WB Mouse IgG1 AA 2-466 Log in to see 4F2E9 2
0.0012584153 ABIN969453 IHC ELISA WB Mouse IgG1 Log in to see 9E7E7 4
0.0012584153 ABIN2869381 ELISA IHC WB Mouse IgG1 AA 2-466 Log in to see 4F2E9 2
0.0012584153 ABIN1531759 IHC ELISA WB Rabbit IgG pSer83 Log in to see Polyclonal
0.0012584153 ABIN2869230 ELISA IHC WB Mouse IgG1 Log in to see 9E7E7-5G3F10-5G3F10E5 1
0.0012584153 ABIN94494 ICC FACS ELISA WB Mouse IgG1 Log in to see VI-RE-1 1
0.0012584153 ABIN192298 ELISA FACS ICC WB PE Mouse IgG1 Log in to see VI-RE-1 1
0.0012584153 ABIN1558082 ICC FACS ELISA WB APC Mouse IgG1 Log in to see VI-RE-1 1
0.0012584153 ABIN1532789 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012584153 ABIN1574031 IHC ELISA WB Rabbit IgG AA 300-350 Log in to see Polyclonal 4
0.0012584153 ABIN269144 ICC FACS IF ELISA WB Mouse IgG1 Log in to see VI-RE-1
0.0012584153 ABIN1501715 IHC ELISA WB Mouse IgG1 Log in to see 9E7E7-
0.0012584153 ABIN957763 IHC (p) IP ELISA WB Mouse IgG1, kappa Log in to see 33C7
0.0012584153 ABIN2888937 ELISA WB Rabbit IgG AA 56-105 Log in to see Polyclonal
0.0012584153 ABIN2889287 IHC (p) ELISA WB Rabbit IgG AA 411-460 Log in to see Polyclonal
0.0012584153 ABIN791058 IHC ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012584153 ABIN2464120 ELISA Mouse IgG1 truncated Log in to see 9E7E7-5G3F10
0.0012584153 ABIN2888938 IHC (p) ELISA WB Rabbit IgG AA 31-80 Log in to see Polyclonal

Top referenced anti-Vimentin antibody for ELISA

Similar anti-Vimentin Antibodies

Application / Reactivity Human
ELISA 216 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 228 Antibodies
Immunochromatography (IC) 12 Antibodies
Immunocytochemistry (ICC) 231 Antibodies
Immunodiffusion (ID) 3 Antibodies
Immunoelectrophoresis (IEP) 3 Antibodies
Immunofluorescence (IF) 277 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 25 Antibodies
Immunohistochemistry (IHC) 277 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 115 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 272 Antibodies
Immunoprecipitation (IP) 98 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Luminex Assay (LMNX) 8 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Epitope pSer56
(23), (17), (16), (16), (16), (15), (14), (12), (12), (8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(806), (294), (254), (205), (118), (110), (95), (77), (75), (42), (39), (32), (30), (29), (25), (7), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(519), (277), (30), (15), (12), (7), (5)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(27), (26), (23), (18), (14), (13), (12), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(671), (299), (290), (290), (239), (233), (232), (128), (104), (25), (15), (12), (8), (8), (5), (3), (3), (3), (3), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1531760) Images
Specificity Vimentin (Phospho-Ser56) Antibody detects endogenous levels of Vimentin only when phosphorylated at Ser56.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Vimentin around the phosphorylation site of Ser56.
Isotype IgG

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN1531760) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Molecular weight of target protein: 53 kDa
Synonyms: VIME, vimentin
Molecular Weight 53 kDa
Gene ID 7431
OMIM 193060
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN1531760) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.642813 (NCBI Gene Symbol: VIM)

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN1531760) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-Vimentin Antibody (ABIN1531760)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Rasmussen, Ji, Eddes, Moritz, Reid, Simpson, Dorow: "Two-dimensional electrophoretic analysis of human breast carcinoma proteins: mapping of proteins that bind to the SH3 domain of mixed lineage kinase MLK2." in: Electrophoresis, Vol. 18, Issue 3-4, pp. 588-98, 1997

Gupta, Aubin, Waye: "Isolation of a human vimentin cDNA with a long 3'-noncoding region from a human osteosarcoma cell line (MG-63)." in: Gene, Vol. 86, Issue 2, pp. 303-4, 1990

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1531760) back to top
Supplier Images
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (pSer56) (ABIN1531760) Western blot analysis of extracts from A549 cells treated with Nocodazole 1ug/ml 16h,...
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (pSer56) (ABIN1531760) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vime...