anti-Human Vimentin antibody for ELISA

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Epitope
pSer56
23
22
18
17
17
16
16
15
13
9
7
7
6
6
6
5
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
880
366
316
220
138
132
118
83
81
52
50
43
41
37
30
12
8
6
5
5
5
3
3
2
2
1
1
1
1
1
Host
Rabbit
537
309
30
14
12
9
8
6
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
28
27
24
21
15
14
12
11
11
11
11
11
11
11
11
10
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
696
313
289
277
246
232
222
114
86
40
15
14
13
10
8
8
5
3
3
3
1
1
1
1
1
Options
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Catalog No. ABIN1531760
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1532790 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN1533459 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN969454 IHC ELISA WB Mouse IgG1 AA 2-466 Log in to see 4F2E9 2
1 ABIN2869381 ELISA IHC WB Mouse IgG1 AA 2-466 Log in to see 4F2E9 2
1 ABIN969453 IHC ELISA WB Mouse IgG1 Log in to see 9E7E7 4
1 ABIN2869230 ELISA IHC WB Mouse IgG1 Log in to see 9E7E7-5G3F10-5G3F10E5 1
1 ABIN94494 ICC FACS ELISA WB Mouse IgG1 Log in to see VI-RE-1 1
1 ABIN192298 ELISA FACS ICC WB PE Mouse IgG1 Log in to see VI-RE-1 1
1 ABIN1531759 IHC ELISA WB Rabbit IgG pSer83 Log in to see Polyclonal
1 ABIN1558082 ICC FACS ELISA WB APC Mouse IgG1 Log in to see VI-RE-1 1
1 ABIN1574031 IHC ELISA WB Rabbit IgG AA 300-350 Log in to see Polyclonal 4
1 ABIN1532789 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN3187472 ELISA IF IHC WB Rabbit IgG Tyr317 Log in to see Polyclonal
1 ABIN3187474 ELISA WB Rabbit IgG Tyr321 Log in to see Polyclonal
1 ABIN3187473 ELISA IF IHC WB Rabbit IgG Tyr319 Log in to see Polyclonal
1 ABIN3182648 ELISA IHC WB Rabbit IgG pSer56 Log in to see Polyclonal
1 ABIN3182647 ELISA IHC WB Rabbit IgG pSer83 Log in to see Polyclonal
1 ABIN269144 CyTOF ELISA FACS ICC IF WB Mouse IgG1 Log in to see VI-RE-1
1 ABIN3187471 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2464120 ELISA Mouse IgG1 truncated Log in to see 9E7E7-5G3F10

Top referenced anti-Vimentin antibody for ELISA

Similar anti-Vimentin Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies
ELISA 233 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 228 Antibodies
Immunochromatography (IC) 12 Antibodies
Immunocytochemistry (ICC) 214 Antibodies
Immunodiffusion (ID) 3 Antibodies
Immunoelectrophoresis (IEP) 3 Antibodies
Immunofluorescence (IF) 267 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 264 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 107 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 304 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Epitope pSer56
(23), (22), (18), (17), (17), (16), (16), (15), (13), (9), (7), (7), (6), (6), (6), (5), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(880), (366), (316), (220), (138), (132), (118), (83), (81), (52), (50), (43), (41), (37), (30), (12), (8), (6), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(537), (309), (30), (14), (12), (9), (8), (6)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(28), (27), (24), (21), (15), (14), (12), (11), (11), (11), (11), (11), (11), (11), (11), (10), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(696), (313), (289), (277), (246), (232), (222), (114), (86), (40), (15), (14), (13), (10), (8), (8), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN1531760) Images
Specificity Vimentin (Phospho-Ser56) Antibody detects endogenous levels of Vimentin only when phosphorylated at Ser56.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Vimentin around the phosphorylation site of Ser56.
Isotype IgG

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN1531760) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Molecular weight of target protein: 53 kDa
Synonyms: VIME, vimentin
Molecular Weight 53 kDa
Gene ID 7431
OMIM 193060
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling ProductDetails: References for anti-Vimentin antibody (ABIN1531760) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.642813 (NCBI Gene Symbol: VIM)

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details ProductDetails: References for anti-Vimentin antibody (ABIN1531760) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

ProductDetails: References for anti-Vimentin antibody (ABIN1531760)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Rasmussen, Ji, Eddes, Moritz, Reid, Simpson, Dorow: "Two-dimensional electrophoretic analysis of human breast carcinoma proteins: mapping of proteins that bind to the SH3 domain of mixed lineage kinase MLK2." in: Electrophoresis, Vol. 18, Issue 3-4, pp. 588-98, 1997

Gupta, Aubin, Waye: "Isolation of a human vimentin cDNA with a long 3'-noncoding region from a human osteosarcoma cell line (MG-63)." in: Gene, Vol. 86, Issue 2, pp. 303-4, 1990

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN1531760) back to top
Supplier Images
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (pSer56) (ABIN1531760) Western blot analysis of extracts from A549 cells treated with Nocodazole 1ug/ml 16h,...
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (pSer56) (ABIN1531760) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vime...