anti-Human Vimentin antibody for Intracellular Staining

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Human
923
401
351
221
138
132
121
83
81
52
51
43
41
37
30
12
8
7
5
5
4
3
3
2
2
1
1
1
1
1
Host
Mouse
570
318
30
14
12
9
9
6
Clonality
Monoclonal
Conjugate
This Vimentin antibody is conjugated to FITC
29
28
25
23
17
16
13
13
13
13
13
13
13
13
12
10
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Intracellular Staining (ICS), Immunohistochemistry (IHC)
736
342
326
305
253
249
249
143
87
40
15
14
13
10
8
7
5
3
3
2
1
1
1
1
1
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Catalog No. ABIN1003466
$ 478.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1003465 ICS IHC Biotin Mouse IgG1 Log in to see hVimentin
1 ABIN1003464 ICS IP IHC ELISA WB Mouse IgG1 Log in to see hVimentin

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human
(923), (401), (351), (221), (138), (132), (121), (83), (81), (52), (51), (43), (41), (37), (30), (12), (8), (7), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(570), (318), (30), (14), (12), (9), (9), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is conjugated to FITC
(29), (28), (25), (23), (17), (16), (13), (13), (13), (13), (13), (13), (13), (13), (12), (10), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Intracellular Staining (ICS), Immunohistochemistry (IHC)
(736), (342), (326), (305), (253), (249), (249), (143), (87), (40), (15), (14), (13), (10), (8), (7), (5), (3), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling Images
Predicted Reactivity Vimentin antibody will recognize most mammalian Vimentins except mouse and rat.
Purification Ion exchange chromatography
Immunogen Purified human Vimentin
Clone HVimentin
Isotype IgG2

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGF-beta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines.
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Application Notes Immunohistochemistry, intracellular staining. For intracellular staining, use 5-10 µl/10⁶ cells. Wash twice with PBS/azide before adding the appropriate amount of secondary fluorescent reagent. Antibody might also be suited for other applications not tested so far.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
Format Lyophilized
Handling Advice Please Note: Always centrifuge product briefly before opening vial. Reconstitute with 0.5 ml sterile H20 or PBS to give a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Protein concentration: 1 mg/ml (after reconstitution).
Storage 4 °C/-20 °C