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anti-Human Vimentin antibody for Immunofluorescence

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Epitope
pSer55
21
16
16
15
14
14
13
13
12
8
5
4
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
576
165
147
73
53
42
26
25
15
11
9
9
8
6
5
4
4
3
2
2
1
1
1
1
1
1
1
Host
Mouse
310
261
30
12
11
6
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
23
21
13
10
9
5
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
477
238
185
142
122
85
85
52
47
23
15
11
8
8
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN1109485
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3140218 ABIN1449160 IF WB Rat IgG2a pSer50 Log in to see TM50 7
0.3140218 ABIN1109486 IF WB Rat IgG2a Log in to see TM71 9
0.3140218 ABIN1109484 IF WB Rat IgG2a Log in to see TM38 3
0.3140218 ABIN1109487 IF WB Mouse IgG2b Log in to see MO82 14
0.3140218 ABIN1449152 IF WB Mouse IgG1 pSer33 Log in to see YT33 2
0.3140218 ABIN268427 ICC IF IHC (p) IHC WB Chicken IgY Log in to see Polyclonal 2
0.3140218 ABIN1981416 FACS IF IHC WB Rabbit IgG AA 430-457, C-Term Log in to see Polyclonal
0.3140218 ABIN1881993 FACS IF IHC WB Rabbit Log in to see Polyclonal 3
0.3140218 ABIN2854814 ICC IHC (fro) IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.3140218 ABIN1580467 ICC IF IHC WB Mouse IgG1 Log in to see 2A52 2
0.3140218 ABIN2705329 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.3140218 ABIN2707782 IC IF WB Rabbit N-Term Log in to see Polyclonal
0.3140218 ABIN1580468 ICC IF IHC WB Mouse IgG2a Log in to see 2D1 2
0.3140218 ABIN955516 IF IHC (p) WB Chicken Ig Log in to see Polyclonal
0.3140218 ABIN2493867 IF WB Mouse Log in to see
0.3140218 ABIN1741989 IF WB Mouse Log in to see 2D1
0.3140218 ABIN2493868 IF WB Chicken Log in to see Polyclonal
0.3140218 ABIN563373 IF IP ELISA WB Mouse IgG2a kappa AA 41-140, partial Log in to see 3E9
0.3140218 ABIN1741988 IF WB Chicken Log in to see Polyclonal
0.3140218 ABIN2405544 IF WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Vimentin antibody for Immunofluorescence

Similar anti-Vimentin Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 449 Antibodies 125 Antibodies 134 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 8 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Immunoprecipitation (IP) 42 Antibodies 8 Antibodies 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 133 Antibodies 46 Antibodies 36 Antibodies
Immunohistochemistry (IHC) 224 Antibodies 57 Antibodies 64 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 40 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Epitope pSer55
(21), (16), (16), (15), (14), (14), (13), (13), (12), (8), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(576), (165), (147), (73), (53), (42), (26), (25), (15), (11), (9), (9), (8), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(310), (261), (30), (12), (11), (6), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(23), (21), (13), (10), (9), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(477), (238), (185), (142), (122), (85), (85), (52), (47), (23), (15), (11), (8), (8), (3), (3), (3), (3), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1109485) Images
Sequence SLYSS-phosphoS55-PGGAYC-KLH
Specificity This antibody reacts specifically with the phosphorylated MPV55 peptide but not the non-phosphorylated peptide. This antibody detects vimentin phosphorylated by cdc2 kinase and does not detect non-phosphorylated vimentin or phosphorylated vimentin by cAMP-dependent kinase, protein kinase C, or Ca2+-calmodulin-dependent protein kinase II on Western blotting.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Protein A agarose
Immunogen Synthetic MPV55 phosphopeptide corresponding to mouse phosphorylated vimentin Ser55.
Clone 4A4
Isotype IgG2b

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN1109485) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is an intermediate filament protein distributed widely in the cytoplasm and is phosphorylated by several protein kinase in vitro. Ser55 residues on vimentin were reported to be one of the phosphorylation sites of vimentin at metaphase and were the phosphorylation sites for cdc2 kinase but not for cAMP-dependent protein kinase, protein kinase C, and Ca2+-calmodulin-dependent protein kinase II in vitro. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and de-phosphorylated in cytokinesis. The use of this antibody that specifically reacts with the phosphorylation site of vimentin Ser55 by cdc2 kinase enables estimation of a particular cdc2 kinase function.Synonyms: VIM
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN1109485) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN1109485) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.

References for anti-Vimentin Antibody (ABIN1109485)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Tatsumi, Sugimoto, Yugawa, Narisawa-Saito, Kiyono, Fujita: "Deregulation of Cdt1 induces chromosomal damage without rereplication and leads to chromosomal instability." in: Journal of cell science, Vol. 119, Issue Pt 15, pp. 3128-40, 2006

Oguri, Inoko, Shima, Izawa, Arimura, Yamaguchi, Inagaki, Kaibuchi, Kikuchi, Inagaki: "Vimentin-Ser82 as a memory phosphorylation site in astrocytes." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 5, pp. 531-40, 2006

Shiromizu, Goto, Tomono, Bartek, Totsukawa, Inoko, Nakanishi, Matsumura, Inagaki: "Regulation of mitotic function of Chk1 through phosphorylation at novel sites by cyclin-dependent kinase 1 (Cdk1)." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 5, pp. 477-85, 2006

Kohn, Aladjem, Weinstein, Pommier: "Molecular interaction maps of bioregulatory networks: a general rubric for systems biology." in: Molecular biology of the cell, Vol. 17, Issue 1, pp. 1-13, 2005

Tsai, Chen, Kriegstein, Vallee: "LIS1 RNA interference blocks neural stem cell division, morphogenesis, and motility at multiple stages." in: The Journal of cell biology, Vol. 170, Issue 6, pp. 935-45, 2005

Englund, Fink, Lau, Pham, Daza, Bulfone, Kowalczyk, Hevner: "Pax6, Tbr2, and Tbr1 are expressed sequentially by radial glia, intermediate progenitor cells, and postmitotic neurons in developing neocortex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 1, pp. 247-51, 2005

Miyata, Kawaguchi, Saito, Kawano, Muto, Ogawa: "Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells." in: Development (Cambridge, England), Vol. 131, Issue 13, pp. 3133-45, 2004

Nakamura, Hashimoto, Amano, Nagata, Matsumoto, Goto, Fukusho, Mori, Kashiwagi, Kudo, Inagaki, Takeda: "Localized phosphorylation of vimentin by rho-kinase in neuroblastoma N2a cells." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 5, Issue 10, pp. 823-37, 2000

Inada, Togashi, Nakamura, Kaibuchi, Nagata, Inagaki: "Balance between activities of Rho kinase and type 1 protein phosphatase modulates turnover of phosphorylation and dynamics of desmin/vimentin filaments." in: The Journal of biological chemistry, Vol. 274, Issue 49, pp. 34932-9, 2000

Goto, Kosako, Tanabe, Yanagida, Sakurai, Amano, Kaibuchi, Inagaki: "Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis." in: The Journal of biological chemistry, Vol. 273, Issue 19, pp. 11728-36, 1998

Scholey: "Kinesin-II, a membrane traffic motor in axons, axonemes, and spindles." in: The Journal of cell biology, Vol. 133, Issue 1, pp. 1-4, 1996

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1109485) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (pSer55) (ABIN1109485) anti-Vimentin (VIM) (pSer55) antibody
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (pSer55) (ABIN1109485) anti-Vimentin (VIM) (pSer55) antibody (Image 2)