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anti-Human Vimentin antibody for Immunohistochemistry (Frozen Sections)

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Reactivity
Chicken, Dog (Canine), Goat, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
578
167
148
73
54
41
25
24
14
11
9
8
8
5
5
4
4
3
2
2
1
1
1
1
1
1
1
Host
Mouse
312
261
30
12
11
6
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
23
21
14
10
9
5
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
479
238
185
141
124
87
84
51
47
23
15
11
8
8
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN112354
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000877444 ABIN112306 EIA IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see VIM 3B4 10
0.000877444 ABIN112184 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see V9 5
0.000877444 ABIN1109490 IHC (fro) IHC (p) WB Mouse IgG1 Log in to see V9 1
0.000877444 ABIN112307 EIA FACS IHC (fro) IHC (p) Biotin Mouse IgG2a Log in to see VIM 3B4 12
0.000877444 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11
0.000877444 ABIN1109489 IHC (fro) WB Mouse IgG1 Log in to see 65E 5
0.000877444 ABIN372741 IHC (fro) IF WB Chicken Ig Log in to see Polyclonal 3
0.000877444 ABIN959503 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see RV202
0.000877444 ABIN959504 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see RV203
0.000877444 ABIN959513 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see V9
0.000877444 ABIN126094 IHC (fro) IHC (p) WB Guinea Pig Log in to see Polyclonal 2
0.000877444 ABIN2854814 ICC IHC (fro) IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.000877444 ABIN335272 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV203 1
0.000877444 ABIN335273 ICC FACS IHC (fro) IHC (p) IHC WB Mouse IgG1 Log in to see V9 7
0.000877444 ABIN335271 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
0.000877444 ABIN204914 ICC FACS IHC (fro) IHC (p) WB Mouse IgG1 pSer72 Log in to see
0.000877444 ABIN335380 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
0.000877444 ABIN335381 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV203 1
0.000877444 ABIN335382 ICC FACS IHC (fro) IHC (p) IHC WB Mouse IgG1 Log in to see V9 7
0.000877444 ABIN1042494 ICC IHC (fro) FACS IHC (p) WB HRP Mouse IgG1 Log in to see RV202 5

Top referenced anti-Vimentin antibody for Immunohistochemistry (Frozen Sections)

Similar anti-Vimentin Antibodies

Application / Reactivity Human Pig (Porcine) Mouse (Murine) Rat (Rattus) Chicken Monkey Dog (Canine) Goat Hamster Zebrafish (Danio rerio)
Western Blotting (WB) 451 Antibodies 48 Antibodies 126 Antibodies 136 Antibodies 33 Antibodies 21 Antibodies 20 Antibodies 13 Antibodies 9 Antibodies 5 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 8 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Immunoprecipitation (IP) 42 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Dog (Canine), Goat, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
(578), (167), (148), (73), (54), (41), (25), (24), (14), (11), (9), (8), (8), (5), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(312), (261), (30), (12), (11), (6), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(23), (21), (14), (10), (9), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(479), (238), (185), (141), (124), (87), (84), (51), (47), (23), (15), (11), (8), (8), (3), (3), (3), (3), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN112354) Images
Specificity RV202 reacts exclusively with Vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Cross-Reactivity (Details) Species reactivity (tested):Canine, Chicken, Goat, Hamster, Human, Monkey, Mouse, Rat, Swine, Zebrafish.
Purification Purified
Immunogen Cytoskeletal vimentin extract of calf lens.
Clone RV202
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN112354) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.Synonyms: VIM
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN112354) Images back to top
Application Notes RV202 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen tissues and Flow cytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN112354) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the lyophilized antibody at 2-8 °C for one month or at -20 °C for longer. Aliquot and store the reconstituted antibody at -20 °C.

References for anti-Vimentin Antibody (ABIN112354)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, 1988

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987