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anti-Human Vimentin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Reactivity
Human
578
168
149
74
54
42
26
25
15
11
9
9
8
6
5
4
4
3
2
2
1
1
1
1
1
1
1
Host
Rabbit
313
260
30
12
11
6
5
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
23
21
14
10
9
5
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
479
238
185
141
124
87
85
52
47
23
15
11
8
8
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN498219
€ 371.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34979305 ABIN239818 ICC IHC (p) IP WB Mouse IgM Log in to see VI-10
0.0008913903 ABIN357888 EIA IHC (p) WB Rabbit Ig Middle Region Log in to see Polyclonal 3
0.0008913903 ABIN1109488 IF IHC (p) WB Mouse IgG1 Log in to see VMT-24 3
0.0008913903 ABIN112354 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see RV202 5
0.0008913903 ABIN112306 EIA IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see VIM 3B4 10
0.0008913903 ABIN389211 IF IHC (p) WB Rabbit Ig AA 63-90, Ser82 Log in to see Polyclonal 3
0.0008913903 ABIN389213 FACS IHC (p) WB Rabbit Ig AA 152-181, Center Log in to see Polyclonal 3
0.0008913903 ABIN389212 FACS IF IHC (p) WB Rabbit Ig AA 430-457, C-Term Log in to see Polyclonal 4
0.0008913903 ABIN357886 EIA IHC (p) WB Rabbit Ig Log in to see Polyclonal 3
0.0008913903 ABIN1686956 IHC (p) Rabbit IgG Log in to see SP20 24
0.0008913903 ABIN112184 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see V9 5
0.0008913903 ABIN1109490 IHC (fro) IHC (p) WB Mouse IgG1 Log in to see V9 1
0.0008913903 ABIN1686955 FACS IHC (p) WB Rabbit IgG Log in to see SP20 32
0.0008913903 ABIN112307 EIA FACS IHC (fro) IHC (p) Biotin Mouse IgG2a Log in to see VIM 3B4 12
0.0008913903 ABIN672786 IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 416-466 Log in to see Polyclonal 17
0.0008913903 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11
0.0008913903 ABIN266288 IHC (p) IHC Rabbit IgG Log in to see Polyclonal 1
0.0008913903 ABIN271977 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0008913903 ABIN268427 ICC IF IHC (p) IHC WB Chicken IgY Log in to see Polyclonal 2
0.0008913903 ABIN1109482 EIA IHC (p) WB Mouse IgG1 Log in to see 9E7E7 3

Top referenced anti-Vimentin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-Vimentin Antibodies

Application / Reactivity Human
ELISA 169 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 83 Antibodies
Immunochromatography (IC) 12 Antibodies
Immunocytochemistry (ICC) 87 Antibodies
Immunodiffusion (ID) 3 Antibodies
Immunoelectrophoresis (IEP) 3 Antibodies
Immunofluorescence (IF) 120 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 224 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 40 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 133 Antibodies
Immunoprecipitation (IP) 42 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Luminex Assay (LMNX) 8 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human
(578), (168), (149), (74), (54), (42), (26), (25), (15), (11), (9), (9), (8), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(313), (260), (30), (12), (11), (6), (5)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(23), (21), (14), (10), (9), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(479), (238), (185), (141), (124), (87), (85), (52), (47), (23), (15), (11), (8), (8), (3), (3), (3), (3), (1), (1), (1)
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling Images
Specificity This antibody detects endogenous levels of Vimentin protein.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity chromatography using epitope-specific immunogen
Purity > 95 % (by SDS-PAGE)

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.Synonyms: VIM
Molecular Weight approx. 57 kDa
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Application Notes Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Vimentin antibody (VIM) (ABIN498219) Immunohistochemistry analyzes of Vimentin antibody in paraffin-embedded human ...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN498219) Western blot analyzes of Vimentin antibody in extracts from A549 cells.