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anti-Human Vimentin antibody for Western Blotting

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Epitope
pSer50
23
17
16
16
16
15
14
13
12
8
7
7
4
4
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
806
293
253
205
118
110
95
77
75
42
39
32
30
29
25
7
5
4
3
2
2
1
1
1
1
1
1
Host
Rat
519
278
30
14
12
7
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
27
26
23
18
14
13
12
11
11
11
11
11
11
11
11
9
5
5
5
4
3
2
2
2
2
2
2
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
671
300
290
289
239
234
232
128
104
25
15
12
8
8
5
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN1449160
$ 434.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.11646367 ABIN1109485 IF WB Mouse IgG2b pSer55 Log in to see 4A4 11
0.11646367 ABIN2773915 IHC WB Rabbit N-Term Log in to see Polyclonal 1
0.11646367 ABIN2783289 IHC WB Rabbit C-Term Log in to see Polyclonal 1
0.11646367 ABIN1109486 IF WB Rat IgG2a Log in to see TM71 9
0.11646367 ABIN1109484 IF WB Rat IgG2a Log in to see TM38 3
0.11646367 ABIN1109487 IF WB Mouse IgG2b Log in to see MO82 14
0.11646367 ABIN1449152 IF WB Mouse IgG1 pSer33 Log in to see YT33 2
0.11646367 ABIN1531760 IHC ELISA WB Rabbit IgG pSer56 Log in to see Polyclonal 2
0.11646367 ABIN389212 FACS IF IHC (p) WB Rabbit Ig AA 430-457, C-Term Log in to see Polyclonal 4
0.11646367 ABIN389211 IF IHC (p) WB Rabbit Ig AA 63-90, Ser82 Log in to see Polyclonal 3
0.11646367 ABIN389213 FACS IHC (p) WB Rabbit Ig AA 152-181, Center Log in to see Polyclonal 3
0.11646367 ABIN223315 WB Rabbit IgG Log in to see Polyclonal 2
0.11646367 ABIN1532790 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.11646367 ABIN1533459 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.11646367 ABIN969454 IHC ELISA WB Mouse IgG1 AA 2-466 Log in to see 4F2E9 2
0.11646367 ABIN1686955 FACS IHC (p) WB Rabbit IgG Log in to see SP20 32
0.11646367 ABIN268950 ICC IF WB Mouse IgM Log in to see VI-01 5
0.11646367 ABIN152563 ICC IF IHC IHC (fro) IHC (p) WB Chicken IgY Log in to see Polyclonal 18
0.11646367 ABIN441334 ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit IgG Log in to see Polyclonal 2
0.11646367 ABIN394656 IF WB Mouse IgG2a kappa AA 41-141 Log in to see 3E9 5

Top referenced anti-Vimentin antibody for Western Blotting

Similar anti-Vimentin Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Simple Western (SimWes) 5 Antibodies 5 Antibodies 4 Antibodies
Western Blotting (WB) 634 Antibodies 194 Antibodies 228 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 8 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Immunoprecipitation (IP) 98 Antibodies 21 Antibodies 31 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 272 Antibodies 111 Antibodies 76 Antibodies
Immunohistochemistry (IHC) 277 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Epitope pSer50
(23), (17), (16), (16), (16), (15), (14), (13), (12), (8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(806), (293), (253), (205), (118), (110), (95), (77), (75), (42), (39), (32), (30), (29), (25), (7), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rat
(519), (278), (30), (14), (12), (7), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(27), (26), (23), (18), (14), (13), (12), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(671), (300), (290), (289), (239), (234), (232), (128), (104), (25), (15), (12), (8), (8), (5), (3), (3), (3), (3), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1449160) Images
Sequence CPSTSRpSLY SSS
Specificity This antibody reacts with vimentin phosphorylated at Ser50.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Protein G agarose
Immunogen KLH-PV50 peptide
Clone TM50
Isotype IgG2a

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN1449160) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Components of intermediate filaments provide information on the origin of vertebrate cells. Antibody to vimentin can be used as to identify the vimentin subclass of intermediate filaments. Vimentin is a ~58 kDa, widely expressed protein that thought to be involved mainly in structural processes, such as wound healing. Scientists have also recently determined that activated human macrophages secrete vimentin into the extracellular space, and overproduction of vimentin has been associated with cellular senescence.Synonyms: VIM
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN1449160) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN1449160) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Vimentin Antibody (ABIN1449160)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Oguri, Inoko, Shima, Izawa, Arimura, Yamaguchi, Inagaki, Kaibuchi, Kikuchi, Inagaki: "Vimentin-Ser82 as a memory phosphorylation site in astrocytes." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 5, pp. 531-40, 2006

Utsugi, Minemura, Hirata, Abe, Watanabe, Ohya: "Movement of yeast 1,3-beta-glucan synthase is essential for uniform cell wall synthesis." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 7, Issue 1, pp. 1-9, 2002

Yanai, Saito, Kakinuma, Kon, Hirota, Taniguchi-Yanai, Nishijo, Shigematsu, Horiguchi, Kasuya, Sugiyama, Yagami, Murakami, Fukamizu: "Renin-dependent cardiovascular functions and renin-independent blood-brain barrier functions revealed by renin-deficient mice." in: The Journal of biological chemistry, Vol. 275, Issue 1, pp. 5-8, 2000

Nakamura, Hashimoto, Amano, Nagata, Matsumoto, Goto, Fukusho, Mori, Kashiwagi, Kudo, Inagaki, Takeda: "Localized phosphorylation of vimentin by rho-kinase in neuroblastoma N2a cells." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 5, Issue 10, pp. 823-37, 2000

Goto, Kosako, Tanabe, Yanagida, Sakurai, Amano, Kaibuchi, Inagaki: "Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis." in: The Journal of biological chemistry, Vol. 273, Issue 19, pp. 11728-36, 1998

Krueger, Daaka, Pitcher, Lefkowitz: "The role of sequestration in G protein-coupled receptor resensitization. Regulation of beta2-adrenergic receptor dephosphorylation by vesicular acidification." in: The Journal of biological chemistry, Vol. 272, Issue 1, pp. 5-8, 1997

Scholey: "Kinesin-II, a membrane traffic motor in axons, axonemes, and spindles." in: The Journal of cell biology, Vol. 133, Issue 1, pp. 1-4, 1996

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1449160) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (pSer50) (ABIN1449160) anti-Vimentin (VIM) (pSer50) antibody
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (pSer50) (ABIN1449160) anti-Vimentin (VIM) (pSer50) antibody (Image 2)