anti-Mammalian Vimentin antibody for Immunocytochemistry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Human, Mammalian, Mouse (Murine), Rat (Rattus)
880
365
315
220
138
132
118
83
81
52
50
43
40
37
30
12
8
6
5
5
5
3
3
2
2
1
1
1
1
1
Host
Mouse
536
310
30
14
12
9
8
6
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
28
27
24
21
15
14
12
11
11
11
11
11
11
11
11
10
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
696
313
290
276
247
232
221
114
86
40
15
14
13
10
8
8
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN268950
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN94491 ICC WB Mouse IgM Log in to see VI-01 4
1 ABIN1580467 ICC IF IHC WB Mouse IgG1 Log in to see 2A52 2
1 ABIN1580468 ICC IF IHC WB Mouse IgG2a Log in to see 2D1 2
1 ABIN4365308 ICC IF IHC SimWes WB Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365341 ICC IF WB PerCP Mouse IgM Log in to see VI-01
1 ABIN4365354 ICC IF WB DyLight 755 Mouse IgM Log in to see VI-01
1 ABIN4365359 ICC IF IHC WB DyLight 680 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365402 ICC IF WB Alexa Fluor 700 Mouse IgM Log in to see VI-01
1 ABIN4365323 ICC IF IHC WB DyLight 405 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365390 ICC IF WB Alexa Fluor 488 Mouse IgM Log in to see VI-01
1 ABIN4365331 ICC IF WB DyLight 405 Mouse IgM Log in to see VI-01
1 ABIN4365360 ICC IF WB DyLight 680 Mouse IgM Log in to see VI-01
1 ABIN4365366 ICC IF IHC WB DyLight 488 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365363 ICC IF WB DyLight 488 Mouse IgM Log in to see VI-01
1 ABIN4365307 ICC IF IHC SimWes WB Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365396 ICC IF WB Alexa Fluor 647 Mouse IgM Log in to see VI-01
1 ABIN4365401 ICC IF IHC WB Alexa Fluor 647 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365407 ICC IF IHC WB Alexa Fluor 700 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365328 ICC IF IHC WB DyLight 350 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365344 ICC IF WB PerCP Mouse IgG2a kappa Log in to see 2D1

Top referenced anti-Vimentin antibody for Immunocytochemistry

Similar anti-Vimentin Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Mammalian
Immunofluorescence (fixed cells) (IF/ICC) 13 Antibodies 8 Antibodies 8 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies 5 Antibodies 3 Antibodies
Simple Western (SimWes) 5 Antibodies 5 Antibodies 2 Antibodies 4 Antibodies
Western Blotting (WB) 37 Antibodies 666 Antibodies 235 Antibodies 274 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Mammalian, Mouse (Murine), Rat (Rattus)
(880), (365), (315), (220), (138), (132), (118), (83), (81), (52), (50), (43), (40), (37), (30), (12), (8), (6), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(536), (310), (30), (14), (12), (9), (8), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(28), (27), (24), (21), (15), (14), (12), (11), (11), (11), (11), (11), (11), (11), (11), (10), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(696), (313), (290), (276), (247), (232), (221), (114), (86), (40), (15), (14), (13), (10), (8), (8), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN268950) Images
Specificity The antibody VI-01 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.
Purification Ammonium sulfate precipitation
Immunogen Pellet of pig brain cold stable proteins after depolymerization of microtubules (NM_003380.3).
Clone VI-01
Isotype IgM

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN268950) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Gene Symbol: VIM
Gene ID 7431
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling ProductDetails: References for anti-Vimentin antibody (ABIN268950) Images back to top
Application Notes Western Blot 1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:2000Immunocytochemistry - Staining technique: RBL rat basophilic leukemia cell line:(a) Fix cells for 10 min in methanol at -20C and for 6 min in acetone at -20C, (b) Fix cells directly in methanol for 10 min at -20oC or in acetone for 10 min at -20oC. Incubation: 45 min RT
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details ProductDetails: References for anti-Vimentin antibody (ABIN268950) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer TBS (approx. pH 8.0)
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

ProductDetails: References for anti-Vimentin antibody (ABIN268950)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Rankin, Tomaszewski, Haase: "Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor." in: Cancer research, Vol. 66, Issue 5, pp. 2576-83, 2006

Deramaudt, Takaoka, Upadhyay, Bowser, Porter, Lee, Rhoades, Johnstone, Weissleder, Hingorani, Mahmood, Rustgi et al.: "N-cadherin and keratinocyte growth factor receptor mediate the functional interplay between Ki-RASG12V and p53V143A in promoting pancreatic cell migration, invasion, and tissue architecture ..." in: Molecular and cellular biology, Vol. 26, Issue 11, pp. 4185-200, 2006

Background publications

Lukás, Dráber, Bucek, Dráberová, Viklický, Dolezel: "Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." in: Histochemistry, Vol. 100, Issue 6, pp. 495-502, 1994

Lukás, Dráber, Bucek, Dráberová, Viklický, Stasková: "Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord." in: The Histochemical journal, Vol. 21, Issue 12, pp. 693-701, 1990

Dráberová, Dráber, Havlícek, Viklický: "A common antigenic determinant of vimentin and desmin defined by monoclonal antibody." in: Folia biologica, Vol. 32, Issue 5, pp. 295-303, 1989

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN268950) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN268950) Immunocytochemistry/Immunofluorescence: Vimentin Antibody (VI-01) [ABIN268950] - Stai...
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN268950) Immunocytochemistry/Immunofluorescence: Vimentin Antibody (VI-01) [ABIN268950] - Stai...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN268950) Western Blot: Vimentin Antibody (VI-01) [ABIN268950] - Analysis of Vimentin in whole ...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN268950) Western Blot: Vimentin Antibody (VI-01) [ABIN268950] - HeLa cell lysate by antibody V...