You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mammalian Vimentin antibody for Immunofluorescence

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Human, Mammalian, Mouse (Murine), Rat (Rattus)
849
366
316
220
138
132
118
83
81
52
50
43
40
37
30
12
8
6
5
5
5
3
3
2
2
1
1
1
1
1
Host
Mouse
523
299
29
14
12
9
8
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
28
27
24
21
15
14
12
11
11
11
11
11
11
11
11
10
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
672
289
289
269
238
227
218
111
82
40
15
14
13
10
8
8
5
3
3
3
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN268950
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.087689 ABIN1580467 ICC IF IHC WB Mouse IgG1 Log in to see 2A52 2
11.087689 ABIN1580468 ICC IF IHC WB Mouse IgG2a Log in to see 2D1 2
1 ABIN372741 IHC (fro) IF WB Chicken Ig Log in to see Polyclonal 3
1 ABIN4365321 ICC IF IHC FITC Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365323 ICC IF IHC WB DyLight 405 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365325 ICC IF WB DyLight 350 Mouse IgM Log in to see VI-01
1 ABIN4365360 ICC IF WB DyLight 680 Mouse IgM Log in to see VI-01
1 ABIN4365363 ICC IF WB DyLight 488 Mouse IgM Log in to see VI-01
1 ABIN4365389 ICC IF IHC WB Alexa Fluor 405 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365402 ICC IF WB Alexa Fluor 700 Mouse IgM Log in to see VI-01
1 ABIN4365344 ICC IF WB PerCP Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365354 ICC IF WB DyLight 755 Mouse IgM Log in to see VI-01
1 ABIN4365353 ICC IF IHC WB DyLight 755 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365359 ICC IF IHC WB DyLight 680 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365366 ICC IF IHC WB DyLight 488 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365384 ICC IF WB Alexa Fluor 405 Mouse IgM Log in to see VI-01
1 ABIN4365390 ICC IF WB Alexa Fluor 488 Mouse IgM Log in to see VI-01
1 ABIN4365328 ICC IF IHC WB DyLight 350 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365331 ICC IF WB DyLight 405 Mouse IgM Log in to see VI-01
1 ABIN4365341 ICC IF WB PerCP Mouse IgM Log in to see VI-01

Top referenced anti-Vimentin antibody for Immunofluorescence

Similar anti-Vimentin Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Mammalian
Immunofluorescence (fixed cells) (IF/ICC) 13 Antibodies 8 Antibodies 8 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies 5 Antibodies 3 Antibodies
Simple Western (SimWes) 5 Antibodies 5 Antibodies 2 Antibodies 4 Antibodies
Western Blotting (WB) 37 Antibodies 642 Antibodies 236 Antibodies 275 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Mammalian, Mouse (Murine), Rat (Rattus)
(849), (366), (316), (220), (138), (132), (118), (83), (81), (52), (50), (43), (40), (37), (30), (12), (8), (6), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(523), (299), (29), (14), (12), (9), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(28), (27), (24), (21), (15), (14), (12), (11), (11), (11), (11), (11), (11), (11), (11), (10), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(672), (289), (289), (269), (238), (227), (218), (111), (82), (40), (15), (14), (13), (10), (8), (8), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN268950) Images
Specificity The antibody VI-01 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.
Purification Ammonium sulfate precipitation
Immunogen Pellet of pig brain cold stable proteins after depolymerization of microtubules (NM_003380.3).
Clone VI-01
Isotype IgM

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN268950) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Gene Symbol: VIM
Gene ID 7431
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN268950) Images back to top
Application Notes Western Blot 1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:2000Immunocytochemistry - Staining technique: RBL rat basophilic leukemia cell line:(a) Fix cells for 10 min in methanol at -20C and for 6 min in acetone at -20C, (b) Fix cells directly in methanol for 10 min at -20oC or in acetone for 10 min at -20oC. Incubation: 45 min RT
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN268950) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer TBS (approx. pH 8.0)
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-Vimentin Antibody (ABIN268950)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Rankin, Tomaszewski, Haase: "Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor." in: Cancer research, Vol. 66, Issue 5, pp. 2576-83, 2006

Deramaudt, Takaoka, Upadhyay, Bowser, Porter, Lee, Rhoades, Johnstone, Weissleder, Hingorani, Mahmood, Rustgi et al.: "N-cadherin and keratinocyte growth factor receptor mediate the functional interplay between Ki-RASG12V and p53V143A in promoting pancreatic cell migration, invasion, and tissue architecture ..." in: Molecular and cellular biology, Vol. 26, Issue 11, pp. 4185-200, 2006

Background publications

Lukás, Dráber, Bucek, Dráberová, Viklický, Dolezel: "Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." in: Histochemistry, Vol. 100, Issue 6, pp. 495-502, 1994

Lukás, Dráber, Bucek, Dráberová, Viklický, Stasková: "Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord." in: The Histochemical journal, Vol. 21, Issue 12, pp. 693-701, 1990

Dráberová, Dráber, Havlícek, Viklický: "A common antigenic determinant of vimentin and desmin defined by monoclonal antibody." in: Folia biologica, Vol. 32, Issue 5, pp. 295-303, 1989

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN268950) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN268950) Immunocytochemistry/Immunofluorescence: Vimentin Antibody (VI-01) [ABIN268950] - Stai...
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN268950) Immunocytochemistry/Immunofluorescence: Vimentin Antibody (VI-01) [ABIN268950] - Stai...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN268950) Western Blot: Vimentin Antibody (VI-01) [ABIN268950] - Analysis of Vimentin in whole ...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN268950) Western Blot: Vimentin Antibody (VI-01) [ABIN268950] - HeLa cell lysate by antibody V...