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anti-Mammalian Vimentin antibody for Immunohistochemistry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Human, Cow (Bovine), Pig (Porcine), Mouse (Murine), Rat (Rattus), Mammalian
849
366
316
219
137
132
118
83
81
52
50
43
40
37
30
12
8
6
5
5
5
3
3
2
2
1
1
1
1
1
Host
Mouse
523
299
29
14
12
9
8
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
28
27
24
21
15
14
12
11
11
11
11
11
11
11
11
10
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
672
289
288
269
238
227
218
111
82
40
15
14
13
10
8
8
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN1580467
$ 220.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1580468 ICC IF IHC WB Mouse IgG2a Log in to see 2D1 2
1 ABIN4365321 ICC IF IHC FITC Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365323 ICC IF IHC WB DyLight 405 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365389 ICC IF IHC WB Alexa Fluor 405 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365353 ICC IF IHC WB DyLight 755 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365359 ICC IF IHC WB DyLight 680 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365366 ICC IF IHC WB DyLight 488 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365328 ICC IF IHC WB DyLight 350 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365401 ICC IF IHC WB Alexa Fluor 647 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365407 ICC IF IHC WB Alexa Fluor 700 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365395 ICC IF IHC WB Alexa Fluor 488 Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365308 ICC IF IHC SimWes WB Mouse IgG2a kappa Log in to see 2D1
1 ABIN4365307 ICC IF IHC SimWes WB Mouse IgG2a kappa Log in to see 2D1
1 ABIN1826492 IHC WB Goat Log in to see Polyclonal
1 ABIN2154614 IHC FITC Mouse IgG2 Log in to see hVimentin
1 ABIN2154613 IHC Biotin Mouse IgG1 Log in to see hVimentin
1 ABIN2154606 IP IHC ELISA WB Mouse IgG1 Log in to see hVimentin
1 ABIN1580469 ICC IF IHC WB Rabbit Log in to see Polyclonal

Similar anti-Vimentin Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Pig (Porcine) Mammalian
Immunofluorescence (fixed cells) (IF/ICC) 13 Antibodies 8 Antibodies 8 Antibodies 1 Antibodies 2 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies 6 Antibodies 5 Antibodies 3 Antibodies 2 Antibodies
Simple Western (SimWes) 5 Antibodies 5 Antibodies 2 Antibodies 4 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 37 Antibodies 642 Antibodies 140 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Cow (Bovine), Pig (Porcine), Mouse (Murine), Rat (Rattus), Mammalian
(849), (366), (316), (219), (137), (132), (118), (83), (81), (52), (50), (43), (40), (37), (30), (12), (8), (6), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(523), (299), (29), (14), (12), (9), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(28), (27), (24), (21), (15), (14), (12), (11), (11), (11), (11), (11), (11), (11), (11), (10), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(672), (289), (288), (269), (238), (227), (218), (111), (82), (40), (15), (14), (13), (10), (8), (8), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1580467) Images
Purification affinity purified antibody
Clone 2A52
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN1580467) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is a protein subunit of 10 nm or intermediate filaments, which are major components of the cytoskeleton in most cell types. Vimentin is the major intemediate filament subunit found in mesenchymal cells, and was first named and characterized in a collaborative study from the labs of German scientists Werner Franke and Klaus Weber. The name derives from the Latin Vimentum, meaning arrays of flexible rods such as in lattices, filigrees and wicker-work, which describes the intermediate filament network quite well. Vimentin is also found in many cell types in tissue culture, most notably fibroblasts, and in developing neuronal and astrocytic precursor cells in the central nervous system. Many cell lines such as Hek293, HeLa, 3T3 and Cos cells contain prominent vimentin networks. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in astrocytes, ependymal cells and neural stem cells), with desmin (in muscle and endothelial cells) and neurofilament proteins (in developing neurons). A E151K point mutation in the vimentin gene was shown to be causative of an autosomal dominant pulverulent cataract disease, but so far only in a single patient. Vimentin is a major protein of eye lens and cornea, and this mutation renders the molecule unable assemble into normal 10 nm filaments. Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. The immunogen used to generate our antibody was recombinant human vimentin expressed in and purified from E. coli. The same immunogen was used to produce our other monoclonal antibody to vimentin MCA-2D1. We also market a very popular chicken polyclonal antibody to vimentin, CPCA-Vim. The HGNC name for this protein is VIM.
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN1580467) Images back to top
Application Notes The antibody solution can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10,000 or lower.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN1580467) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer The antibody solution is affinity purified from tissue culture supernatant and is at concentration of 1mg/ml in phosphate buffered saline preparation containing 10 mM sodium azide preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 4°C short term or -20°C long term.

References for anti-Vimentin Antibody (ABIN1580467)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Müller, Bhattacharya, Moore, Prescott, Wedig, Herrmann, Magin: "Dominant cataract formation in association with a vimentin assembly disrupting mutation." in: Human molecular genetics, Vol. 18, Issue 6, pp. 1052-7, 2009

Franke, Schmid, Osborn, Weber: "Different intermediate-sized filaments distinguished by immunofluorescence microscopy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 75, Issue 10, pp. 5034-8, 1979

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1580467) back to top
Supplier Images
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN1580467) Western blot of crude extract of the human carcinoma HeLa cell line. Lane 18 was prob...
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN1580467) View of mixed neuron/glial cultures stained with CPCA-Vim (green) and ’s rabbit antib...