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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Mammalian|
|Immunofluorescence (fixed cells) (IF/ICC)||13 Antibodies||8 Antibodies||8 Antibodies||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||10 Antibodies||5 Antibodies||3 Antibodies|
|Simple Western (SimWes)||5 Antibodies||5 Antibodies||2 Antibodies||4 Antibodies|
|Western Blotting (WB)||37 Antibodies||648 Antibodies||236 Antibodies||275 Antibodies|
|Transfected Cell Culture (TCC)||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Antigen||Vimentin (VIM) Antibodies|
|Reactivity||Human, Mammalian, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Vimentin antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Product Details anti-Vimentin AntibodyTarget Details Vimentin Application Details Handling Images
|Specificity||Specific for the ~50 kDa Vimentin protein.|
Species reactivity (expected):other Mammalian.
Species reactivity (tested):Human, Mouse and Rat.
|Immunogen||Recombinant human Vimentin purified from E. coli.|
Target Details VimentinProduct Details anti-Vimentin Antibody Application Details Handling Images back to top
|Alternative Name||Vimentin (VIM Antibody Abstract)|
|Background||Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).Synonyms: VIM|
|Research Area||Cell/Tissue Markers, Cytoskeleton, Lineage Markers, Neural Stem Cell marker|
|Pathways||TCR Signaling, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Western blotting: 1/1,000. Immunofluorescence: 1/500. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|