anti-Mammalian Vimentin antibody for Immunohistochemistry (Frozen Sections)

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Human, Mammalian, Mouse (Murine), Rat (Rattus)
923
400
350
221
138
132
121
83
81
52
51
43
40
37
30
12
8
7
5
5
4
3
3
2
2
1
1
1
1
1
Host
Chicken
571
318
29
14
12
9
9
6
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
30
28
25
23
17
16
13
13
13
13
13
13
13
13
12
10
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
735
342
327
304
253
249
249
142
87
40
15
14
13
10
8
7
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN5551655
$ 375.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN471304 IHC (fro) IHC (p) ELISA WB Mouse IgG1 Log in to see

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Mammalian, Mouse (Murine), Rat (Rattus)
(923), (400), (350), (221), (138), (132), (121), (83), (81), (52), (51), (43), (40), (37), (30), (12), (8), (7), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Chicken
(571), (318), (29), (14), (12), (9), (9), (6)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(30), (28), (25), (23), (17), (16), (13), (13), (13), (13), (13), (13), (13), (13), (12), (10), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(735), (342), (327), (304), (253), (249), (249), (142), (87), (40), (15), (14), (13), (10), (8), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling Images
Specificity Specific for the ~50 kDa Vimentin protein.
Cross-Reactivity (Details) Species reactivity (expected):other Mammalian.
Species reactivity (tested):Human, Mouse and Rat.
Purification Ig Fraction
Immunogen Recombinant human Vimentin purified from E. coli.
Isotype Ig Fraction

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).Synonyms: VIM
Gene ID 81818
NCBI Accession NP_112402
UniProt P31000
Research Area Cell/Tissue Markers, Cytoskeleton, Lineage Markers, Neural Stem Cell marker
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Application Notes Western blotting: 1/1,000. Immunofluorescence: 1/500. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.