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anti-Mammalian Vimentin antibody for Immunohistochemistry (Frozen Sections)

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Human, Mammalian, Mouse (Murine), Rat (Rattus)
857
370
316
221
139
132
115
84
79
50
49
42
40
36
30
10
7
7
5
5
4
3
3
2
2
2
1
1
1
1
1
1
Host
Chicken
530
302
29
15
12
8
7
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
28
28
23
20
15
14
12
11
11
11
11
11
11
11
11
9
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
683
301
283
266
249
221
217
107
84
40
15
14
13
10
8
8
5
3
3
3
3
1
1
1
1
1
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Catalog No. ABIN372741
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN471304 IHC (fro) IHC (p) ELISA WB Mouse IgG1 Log in to see

Top referenced anti-Vimentin antibody for Immunohistochemistry (Frozen Sections)

Similar anti-Vimentin Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Mammalian
Immunofluorescence (fixed cells) (IF/ICC) 13 Antibodies 8 Antibodies 8 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies 5 Antibodies 3 Antibodies
Simple Western (SimWes) 5 Antibodies 5 Antibodies 2 Antibodies 4 Antibodies
Western Blotting (WB) 37 Antibodies 653 Antibodies 238 Antibodies 281 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Mammalian, Mouse (Murine), Rat (Rattus)
(857), (370), (316), (221), (139), (132), (115), (84), (79), (50), (49), (42), (40), (36), (30), (10), (7), (7), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Chicken
(530), (302), (29), (15), (12), (8), (7)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(28), (28), (23), (20), (15), (14), (12), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(683), (301), (283), (266), (249), (221), (217), (107), (84), (40), (15), (14), (13), (10), (8), (8), (5), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN372741) Images
Specificity Specific for the ~50 kDa Vimentin protein.
Cross-Reactivity (Details) Species reactivity (expected):other Mammalian.
Species reactivity (tested):Human, Mouse and Rat.
Purification Ig Fraction
Immunogen Recombinant human Vimentin purified from E. coli.
Isotype Ig

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN372741) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).Synonyms: VIM
Gene ID 81818
NCBI Accession NP_112402
UniProt P31000
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN372741) Images back to top
Application Notes Western blotting: 1/1,000. Immunofluorescence: 1/500. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN372741) Images back to top
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-Vimentin Antibody (ABIN372741)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Kirane, Toombs, Ostapoff, Carbon, Zaknoen, Braunfeld, Schwarz, Burrows, Brekken: "Apricoxib, a novel inhibitor of COX-2, markedly improves standard therapy response in molecularly defined models of pancreatic cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 18, Issue 18, pp. 5031-42, 2012

Background publications

Zhao, Yan, Long, Chen, Wang: "Vimentin affects the mobility and invasiveness of prostate cancer cells." in: Cell biochemistry and function, Vol. 26, Issue 5, pp. 571-7, 2008

Nieminen, Henttinen, Merinen, Marttila-Ichihara, Eriksson, Jalkanen: "Vimentin function in lymphocyte adhesion and transcellular migration." in: Nature cell biology, Vol. 8, Issue 2, pp. 156-62, 2006

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN372741) back to top
Supplier Images
 image for anti-Vimentin antibody (VIM) (ABIN372741) anti-Vimentin (VIM) antibody
 image for anti-Vimentin antibody (VIM) (ABIN372741) anti-Vimentin (VIM) antibody (Image 2)