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anti-Mammalian Vimentin antibody for Western Blotting

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Reactivity
Mammalian
577
166
148
73
53
42
26
25
15
10
9
9
8
6
5
4
4
3
2
2
1
1
1
1
1
1
1
Host
Mouse
310
261
30
12
11
6
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
23
21
13
10
9
5
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Western Blotting (WB)
477
238
185
142
123
85
84
52
47
23
15
11
8
8
3
3
3
3
1
1
1
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Catalog No. ABIN94491
$ 265.65
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN1580467 ICC IF IHC WB Mouse IgG1 Log in to see 2A52 2
0.12303223 ABIN1580468 ICC IF IHC WB Mouse IgG2a Log in to see 2D1 2
0.0011806369 ABIN372741 IHC (fro) IF WB Chicken Ig Log in to see Polyclonal 3
0.0011806369 ABIN3200819 ICC WB Mouse IgM Log in to see VI-01
0.0011806369 ABIN2154606 IP IHC ELISA WB Mouse IgG1 Log in to see hVimentin
0.0011806369 ABIN1580469 ICC IF IHC WB Rabbit Log in to see Polyclonal
0.0011806369 ABIN3040254 ICC IF WB Mouse IgM Log in to see

Top referenced anti-Vimentin antibody for Western Blotting

Similar anti-Vimentin Antibodies

Application / Reactivity Mammalian
ELISA 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunohistochemistry (IHC) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 8 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Mammalian
(577), (166), (148), (73), (53), (42), (26), (25), (15), (10), (9), (9), (8), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(310), (261), (30), (12), (11), (6), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(23), (21), (13), (10), (9), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Western Blotting (WB)
(477), (238), (185), (142), (123), (85), (84), (52), (47), (23), (15), (11), (8), (8), (3), (3), (3), (3), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN94491) Images
Specificity The antibody VI-01 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.
Purification Purified from ascites by precipitation methods.
Purity > 95 % (by SDS-PAGE)
Immunogen Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
Clone VI-01
Isotype IgM

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN94491) Images back to top
Antigen
Alternative Name VIM (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP).Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin.Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN94491) Images back to top
Application Notes Immunocytochemistry
Staining technique: RBL rat basophilic leukemia cell line:
(a) Fix cells for 10 min in methanol at -20 °C and for 6 min in acetone at -20 °C,
(b) Fix cells directly in methanol for 10 min at -20 °C or in acetone for 10 min at -20 °C.
Incubation: 45 min RT
Positive control: 3T3 mouse Swiss albino fibroblast cell lineRBL rat basophilic leukemia cell line

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN94491) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-Vimentin Antibody (ABIN94491)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Bacáková, Mares, Lisá, Svorcík: "Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions." in: Biomaterials, Vol. 21, Issue 11, pp. 1173-9, 2000

Dráberová, Dráber, Havlícek, Viklický: "A common antigenic determinant of vimentin and desmin defined by monoclonal antibody." in: Folia biologica, Vol. 32, Issue 5, pp. 295-303, 1989

Background publications

Lukás, Dráber, Bucek, Dráberová, Viklický, Dolezel: "Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." in: Histochemistry, Vol. 100, Issue 6, pp. 495-502, 1994

Lukás, Dráber, Bucek, Dráberová, Viklický, Stasková: "Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord." in: The Histochemical journal, Vol. 21, Issue 12, pp. 693-701, 1990

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN94491) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN94491) Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of 3T3 mo...
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN94491) Immunofluorescence staining (rat basophils)
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN94491) Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast...
Immunoprecipitation (IP) image for anti-Vimentin antibody (VIM) (ABIN94491) Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detec...