anti-Monkey Vimentin antibody for Immunohistochemistry (Frozen Sections)

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Chicken, Dog (Canine), Goat, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
924
400
350
220
138
131
120
83
80
52
50
43
41
36
30
12
8
7
5
4
4
3
3
2
2
1
1
1
1
1
Host
Mouse
570
319
30
14
12
9
9
6
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
30
28
25
23
17
16
13
13
13
13
13
13
13
13
12
10
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
736
342
327
304
253
249
248
142
87
40
15
14
13
10
8
7
5
3
3
3
1
1
1
1
1
Supplier
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Catalog No. ABIN112354
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN112306 EIA IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see VIM 3B4 10
1 ABIN959503 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see RV202
1 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11
1 ABIN4365304 ELISA IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 4F2E9 1
1 ABIN1042493 ICC IHC (fro) FACS IHC (p) WB FITC Mouse IgG1 Log in to see RV202 5
1 ABIN1042494 ICC IHC (fro) FACS IHC (p) WB HRP Mouse IgG1 Log in to see RV202 5
1 ABIN335271 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
1 ABIN335380 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
1 ABIN1805483 ICC IHC (fro) FACS FITC Mouse IgG1 Log in to see RV202
1 ABIN1805484 ICC IHC (fro) WB HRP Mouse IgG1 Log in to see RV202
1 ABIN222825 IHC (fro) IF IHC (p) ELISA WB FITC Mouse IgG2a Log in to see
1 ABIN225110 IHC (fro) IHC (p) WB Mouse IgG2a Log in to see
1 ABIN225111 IHC (fro) IF IHC (p) ELISA WB Mouse IgG2a Log in to see
1 ABIN4365303 ELISA IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 4F2E9
1 ABIN5540611 FACS ICC IF IHC (fro) FITC Mouse IgG1 Log in to see RV202
1 ABIN5540612 FACS ICC IF IHC (fro) HRP Mouse IgG1 Log in to see RV202
1 ABIN5618631 FACS IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Dog (Canine), Goat, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
(924), (400), (350), (220), (138), (131), (120), (83), (80), (52), (50), (43), (41), (36), (30), (12), (8), (7), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(570), (319), (30), (14), (12), (9), (9), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(30), (28), (25), (23), (17), (16), (13), (13), (13), (13), (13), (13), (13), (13), (12), (10), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(736), (342), (327), (304), (253), (249), (248), (142), (87), (40), (15), (14), (13), (10), (8), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN112354) Images
Specificity RV202 reacts exclusively with Vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Cross-Reactivity (Details) Species reactivity (tested):Canine, Chicken, Goat, Hamster, Human, Monkey, Mouse, Rat, Swine, Zebrafish.
Purification Purified
Immunogen Cytoskeletal vimentin extract of calf lens.
Clone RV202
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN112354) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.Synonyms: VIM
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN112354) Images back to top
Application Notes RV202 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen tissues and Flow cytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN112354) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the lyophilized antibody at 2-8 °C for one month or at -20 °C for longer. Aliquot and store the reconstituted antibody at -20 °C.

References for anti-Vimentin antibody (ABIN112354)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, 1988

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987