You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Pig (Porcine) Vimentin antibody for Immunofluorescence (Cultured Cells)

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Epitope
AA 416-466
22
22
17
17
16
16
16
15
13
8
7
7
6
6
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
857
370
316
220
139
132
115
84
79
50
49
42
41
36
30
10
7
7
5
5
4
3
3
2
2
2
1
1
1
1
1
1
Host
Rabbit
530
301
30
15
12
8
7
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
28
28
23
20
15
14
12
11
11
11
11
11
11
11
11
9
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
683
301
282
267
249
220
217
108
84
39
15
14
12
10
8
8
5
3
3
3
3
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN672786
$ 307.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2808165 FACS IF (cc) IF (p) Alexa Fluor 594 Rabbit IgG AA 416-466 Log in to see Polyclonal
1 ABIN672793 FACS IF (cc) IF (p) FITC Rabbit IgG AA 416-466 Log in to see Polyclonal
1 ABIN672789 FACS IF (cc) IF (p) Cy3 Rabbit IgG AA 416-466 Log in to see Polyclonal
1 ABIN672792 FACS IF (cc) IF (p) Cy7 Rabbit IgG AA 416-466 Log in to see Polyclonal
1 ABIN672791 FACS IF (cc) IF (p) Cy5.5 Rabbit IgG AA 416-466 Log in to see Polyclonal
1 ABIN672790 FACS IF (cc) IF (p) Cy5 Rabbit IgG AA 416-466 Log in to see Polyclonal
1 ABIN919699 FACS IF (cc) IF (p) Alexa Fluor 555 Rabbit IgG AA 416-466 Log in to see Polyclonal
1 ABIN919696 FACS IF (cc) IF (p) Alexa Fluor 488 Rabbit IgG AA 416-466 Log in to see Polyclonal
1 ABIN919700 FACS IF (cc) IF (p) Alexa Fluor 647 Rabbit IgG AA 416-466 Log in to see Polyclonal
1 ABIN919698 FACS IF (cc) IF (p) Alexa Fluor 350 Rabbit IgG AA 416-466 Log in to see Polyclonal
1 ABIN5012403 FACS IF (cc) IF (p) Alexa Fluor 680 Rabbit IgG AA 416-466 Log in to see Polyclonal
1 ABIN5012404 FACS IF (cc) IF (p) Alexa Fluor 750 Rabbit IgG AA 416-466 Log in to see Polyclonal

Top referenced anti-Vimentin antibody for Immunofluorescence (Cultured Cells)

Similar anti-Vimentin Antibodies

Application / Reactivity Rat (Rattus) Pig (Porcine)
Cytometry by Time of Flight (CyTOF) 5 Antibodies 6 Antibodies
ELISA 47 Antibodies 40 Antibodies
Flow Cytometry (FACS) 91 Antibodies 108 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 137 Antibodies
Immunofluorescence (IF) 151 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 102 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 53 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 138 Antibodies
Immunoprecipitation (IP) 30 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Simple Western (SimWes) 4 Antibodies
Western Blotting (WB) 281 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Epitope AA 416-466
(22), (22), (17), (17), (16), (16), (16), (15), (13), (8), (7), (7), (6), (6), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(857), (370), (316), (220), (139), (132), (115), (84), (79), (50), (49), (42), (41), (36), (30), (10), (7), (7), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(530), (301), (30), (15), (12), (8), (7)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(28), (28), (23), (20), (15), (14), (12), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(683), (301), (282), (267), (249), (220), (217), (108), (84), (39), (15), (14), (12), (10), (8), (8), (5), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 20 references available
Supplier Log in to see

Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN672786) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human Vimentin
Isotype IgG

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN672786) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Subcellular location: Cytoplasm


Synonyms: HEL113, CTRCT3, Vimentin, VIM
Gene ID 7431
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN672786) Images back to top
Application Notes WB(1:100-1000), FCM(1:20-100), IHC-P(1:100-500), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN672786) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-Vimentin Antibody (ABIN672786)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Sucre, Wilkinson, Vijayaraj, Paul, Dunn, Alva-Ornelas, Gomperts: "A three-dimensional human model of the fibroblast activation that accompanies bronchopulmonary dysplasia identifies Notch-mediated pathophysiology." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 310, Issue 10, pp. L889-98, 2016

Huang, Wen: "Phenotype transformation of immortalized NCM460 colon epithelial cell line by TGF-β1 is associated with chromosome instability." in: Molecular biology reports, Vol. 43, Issue 10, pp. 1069-78, 2016

Monteiro, Smith, Angstadt, Zhang, Khademhosseini, Yelick: "Dental cell sheet biomimetic tooth bud model." in: Biomaterials, Vol. 106, pp. 167-79, 2016

Wilkinson, Alva-Ornelas, Sucre, Vijayaraj, Durra, Richardson, Jonas, Paul, Karumbayaram, Dunn, Gomperts: "Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling." in: Stem cells translational medicine, 2016

Tong, Sun, Wang, Suo: "Sorcin Enhances Metastasis and Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer." in: Cell biochemistry and biophysics, 2015

Pan, Li, Duan, Yuan, Fan, Fan, Xiaokaiti, Yang, Wang, Li: "Enoxaparin Sensitizes Human Non-Small-Cell Lung Carcinomas to Gefitinib by Inhibiting DOCK1 Expression, Vimentin Phosphorylation, and Akt Activation." in: Molecular pharmacology, Vol. 87, Issue 3, pp. 378-90, 2015

Cheng, Xiang, Yang, Ma, Zhao: "Direct Effects of Bevacizumab on Rat Conjunctival Fibroblast." in: Cell biochemistry and biophysics, 2015

Deng, Zhang, Zhang, Wen, Miao, Guo: "MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7." in: Tumour biology, 2015

Fernández-Ferreiro, González Barcia, Gil-Martínez, Vieites-Prado, Lema, Argibay, Blanco Méndez, Lamas, Otero-Espinar: "In vitro and in vivo ocular safety and eye surface permanence determination by direct and Magnetic Resonance Imaging of ion-sensitive hydrogels based on gellan gum and kappa-carrageenan." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, Vol. 94, pp. 342-51, 2015

Karim, Reese, Wilson, Jacobson, Zhong, Djamali: "Nox2 Is a Mediator of Ischemia Reperfusion Injury." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 2015

Su, Li: "RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10760-7, 2015

Li, Inoue, Takamatsu, Kobayashi, Shiraishi, Maeda, Ohashi, Nishida: "Differences between niche cells and limbal stromal cells in maintenance of corneal limbal stem cells." in: Investigative ophthalmology & visual science, Vol. 55, Issue 3, pp. 1453-62, 2014

Wang, Hu, Gao, Xu, Ning, Zhang: "Immortalized porcine intestinal epithelial cell cultures susceptible to porcine rotavirus infection." in: Journal of virological methods, Vol. 202, pp. 87-94, 2014

Sottnik, Campbell, Mehra, Behbahani-Nejad, Hall, Keller: "Osteocytes serve as a progenitor cell of osteosarcoma." in: Journal of cellular biochemistry, Vol. 115, Issue 8, pp. 1420-9, 2014

Li, Dong, Wei, Wang, Zhang, Li: "Fatty acid synthase mediates the epithelial-mesenchymal transition of breast cancer cells." in: International journal of biological sciences, Vol. 10, Issue 2, pp. 171-80, 2014

Yang, Hui, Wang, Yang, Jiang: "Overexpression of SOX2 promotes migration, invasion, and epithelial-mesenchymal transition through the Wnt/?-catenin pathway in laryngeal cancer Hep-2 cells." in: Tumour biology, Vol. 35, Issue 8, pp. 7965-73, 2014

Liu, Han, Wang, Feng: "Down-regulation of Wnt10a affects odontogenesis and proliferation in mesenchymal cells." in: Biochemical and biophysical research communications, Vol. 434, Issue 4, pp. 717-21, 2013 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Wang, Wang, Zhao, Li, Deng: "Angiotensin II upregulates K(Ca)3.1 channels and stimulates cell proliferation in rat cardiac fibroblasts." in: Biochemical pharmacology, Vol. 85, Issue 10, pp. 1486-94, 2013

Wang, Huang, Guan, Xiao, Deng, Chen, Chen, Li, Huang, Shi: "Hypoxia-inducible factor-1alpha and MAPK co-regulate activation of hepatic stellate cells upon hypoxia stimulation." in: PLoS ONE, Vol. 8, Issue 9, pp. e74051, 2013

Fu, Chen, Xia, Zhang: "Effects of Ureaplasma urealyticum lipid-associated membrane proteins on rheumatoid arthritis synovial fibroblasts." in: The Journal of international medical research, Vol. 41, Issue 5, pp. 1655-70, 2013

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN672786) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (AA 416-466) (ABIN672786) Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polycl...
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (AA 416-466) (ABIN672786) Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Viment...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (AA 416-466) (ABIN672786) Mouse spleen lysates probed with Anti-Vimentin Polyclonal Antibody, Unconjugated at ...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (AA 416-466) (ABIN672786) 20 μg proteins of cell lysates from U138, HeLa and A431 were separated by SDS-PAGE an...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Vimentin antibody (VIM) (AA 416-466) (ABIN672786) Image provided by One World Lab validation program. U138 cells were stained with bs-0...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Vimentin antibody (VIM) (AA 416-466) (ABIN672786) Image submitted by One World Lab validation program. U138 cells were stained with bs-...
Flow Cytometry (FACS) image for anti-Vimentin antibody (VIM) (AA 416-466) (ABIN672786) Human Jurkat cells probed with Vimentin Polyclonal Antibody, Unconjugated at 0.2ug f...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Vimentin antibody (VIM) (AA 416-466) (ABIN672786) Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Viment...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Vimentin antibody (VIM) (AA 416-466) (ABIN672786) Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polycl...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Vimentin antibody (VIM) (AA 416-466) (ABIN672786) This image was kindly submitted by Preethi Vijayaraj from UCLA, 4% PFA fixed cells, p...