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anti-Potoroo Vimentin antibody for Immunocytochemistry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Reactivity
Human, Rat (Rattus), Chicken, Potoroo
576
165
148
73
53
41
26
25
15
11
9
9
8
6
5
4
4
2
2
2
1
1
1
1
1
1
1
Host
Mouse
310
261
30
12
11
6
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
23
21
13
10
9
5
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
477
237
185
141
123
84
84
51
47
23
15
11
8
8
3
3
3
3
1
1
1
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Catalog No. ABIN335273
$ 221.46
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.001015949 ABIN335382 ICC FACS IHC (fro) IHC (p) IHC WB Mouse IgG1 Log in to see V9 7

Top referenced anti-Vimentin antibody for Immunocytochemistry

Similar anti-Vimentin Antibodies

Application / Reactivity Rat (Rattus) Potoroo
ELISA 32 Antibodies
Flow Cytometry (FACS) 17 Antibodies 3 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 35 Antibodies 2 Antibodies
Immunofluorescence (IF) 52 Antibodies 1 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 64 Antibodies 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 20 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies 3 Antibodies
Immunoprecipitation (IP) 16 Antibodies
Western Blotting (WB) 134 Antibodies 3 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Rat (Rattus), Chicken, Potoroo
(576), (165), (148), (73), (53), (41), (26), (25), (15), (11), (9), (9), (8), (6), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(310), (261), (30), (12), (11), (6), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(23), (21), (13), (10), (9), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(477), (237), (185), (141), (123), (84), (84), (51), (47), (23), (15), (11), (8), (8), (3), (3), (3), (3), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN335273) Images
Specificity Human, rat, chicken and potoroo.
Purification Culture supernatant
Immunogen V9 is a mouse monoclonal IgG1 antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with vimentin isolated from porcine lens.
Clone V9
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN335273) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN335273) Images back to top
Application Notes V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. V9 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer (pH 6.0) is required. Optimal antibody dilution should be determined by titration.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN335273) Images back to top
Format Liquid
Storage 4 °C

References for anti-Vimentin Antibody (ABIN335273)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

De Visscher, Plusquin, Mesure, Flameng: "Selection of an immunohistochemical panel for cardiovascular research in sheep." in: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry, Vol. 18, Issue 4, pp. 382-91, 2010

Corver, Koopman, van der Aa, Regensburg, Fleuren, Cornelisse: "Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer." in: Cytometry, Vol. 39, Issue 2, pp. 96-107, 2000

Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, 1987

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, 1987

Tölle, Weber, Osborn: "Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments." in: European journal of cell biology, Vol. 38, Issue 2, pp. 234-44, 1985

Osborn, Debus, Weber: "Monoclonal antibodies specific for vimentin." in: European journal of cell biology, Vol. 34, Issue 1, pp. 137-43, 1984

Background publications

Brussee, Smit, Koopman, Wijga, Kerkhof, Corver, Vos, Gerritsen, Grobbee, Brunekreef, Merkus, de Jongste: "Interrupter resistance and wheezing phenotypes at 4 years of age." in: American journal of respiratory and critical care medicine, Vol. 169, Issue 2, pp. 209-13, 2004

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN335273) back to top
Supplier Images
 image for anti-Vimentin antibody (VIM) (ABIN335273) anti-Vimentin (VIM) antibody