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|Application / Reactivity||Human||Rat (Rattus)||Pig (Porcine)||Rabbit|
|Simple Western (SimWes)||5 Antibodies||4 Antibodies||2 Antibodies|
|Western Blotting (WB)||634 Antibodies||146 Antibodies||228 Antibodies||4 Antibodies|
|Transfected Cell Culture (TCC)||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Luminex Assay (LMNX)||8 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Immunoprecipitation (IP)||98 Antibodies||64 Antibodies||31 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||272 Antibodies||133 Antibodies||111 Antibodies|
|Antigen||Vimentin (VIM) Antibodies|
|Reactivity||Human, Pig (Porcine), Rabbit, Rat (Rattus) Alternatives|
|Conjugate||This Vimentin antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Vimentin AntibodyTarget Details Vimentin Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Mouse IgG monoclonal antibody for Vimentin, vimentin (VIM) detection. Tested with WB, IHC-P in Human,rat,rabbit,pig.
Gene Name: vimentin
Protein Name: Vimentin
|Immunogen||Pig eye lens vimentin.|
Target Details VimentinProduct Details anti-Vimentin Antibody Application Details Handling Images back to top
|Alternative Name||VIM (VIM Antibody Abstract)|
|Background||The VIM gene was one of many that Gieser and Swaroop(1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.|
|Gene ID||7431, 22352, 81818|
|UniProt||P08670, P20152, P31000|
|Pathways||TCR Signaling, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
WB: Concentration: 0.5-1 μg/mL, Species: Human, rabbit, pig, rat
IHC-P: Concentration: 1.5-2.5 μg/mL, Species: Human, rabbit, pig, rat
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921123 in WB, supported by ABIN921230 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
|Reconstitution||Add 1 mL of PBS buffer will yield a concentration of 100 μg/mL.|
|Buffer||Mouse ascites fluid, 1.2 % sodium acetate, 2 mg BSA, with 0.01 mg Sodium azide as preservative.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling back to top