anti-Rat (Rattus) Vimentin antibody for Immunofluorescence

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Epitope
pSer50
40
24
24
22
18
18
18
15
15
8
7
7
6
6
6
5
5
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
923
400
350
221
138
132
121
83
81
52
51
43
41
37
30
12
8
7
5
5
4
3
3
2
2
1
1
1
1
1
Host
Rat
571
318
30
13
12
9
9
6
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
30
28
25
23
17
16
13
13
13
13
13
13
13
13
12
10
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
735
342
327
304
253
249
249
143
87
40
15
14
13
10
8
7
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN1449160
$ 434.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.17825 ABIN1109485 IF WB Mouse IgG2b pSer55 Log in to see 4A4 11
11.17825 ABIN1109486 IF WB Rat IgG2a Log in to see TM71 9
11.17825 ABIN1109487 IF WB Mouse IgG2b Log in to see MO82 14
11.17825 ABIN269191 ICC IF IHC (p) IP IHC WB Mouse IgM Log in to see VI-10 1
11.17825 ABIN152563 ICC IF IHC IHC (fro) IHC (p) WB Chicken IgY Log in to see Polyclonal 18
11.17825 ABIN2705329 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
11.17825 ABIN268950 ICC IF WB Mouse IgM Log in to see VI-01 5
11.17825 ABIN441334 ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit IgG Center Log in to see Polyclonal 2
11.17825 ABIN261651 ICC IF IHC (p) IHC WB Mouse IgM Log in to see J144 1
11.17825 ABIN2854814 ICC IHC (fro) IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
11.17825 ABIN2707782 IC IF WB Rabbit N-Term Log in to see Polyclonal
11.17825 ABIN1580467 ICC IF IHC WB Mouse IgG1 Log in to see 2A52 2
11.17825 ABIN1580468 ICC IF IHC WB Mouse IgG2a Log in to see 2D1 2
11.17825 ABIN4365451 ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG2a Center Log in to see 7812
11.17825 ABIN1875329 IF IHC WB Rabbit IgG Log in to see Polyclonal
11.17825 ABIN1875330 IF WB Rabbit IgG Log in to see Polyclonal
11.17825 ABIN2852672 IC IF IHC WB Mouse Log in to see
11.17825 ABIN1741989 IF WB Mouse Log in to see 2D1
11.17825 ABIN1741988 IF WB Chicken Log in to see Polyclonal
11.17825 ABIN2405508 IF IHC WB Rabbit IgG Log in to see Polyclonal

General

Antigen Vimentin (VIM) Antibodies
Epitope pSer50
(40), (24), (24), (22), (18), (18), (18), (15), (15), (8), (7), (7), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(923), (400), (350), (221), (138), (132), (121), (83), (81), (52), (51), (43), (41), (37), (30), (12), (8), (7), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rat
(571), (318), (30), (13), (12), (9), (9), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(30), (28), (25), (23), (17), (16), (13), (13), (13), (13), (13), (13), (13), (13), (12), (10), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunofluorescence (IF), Western Blotting (WB)
(735), (342), (327), (304), (253), (249), (249), (143), (87), (40), (15), (14), (13), (10), (8), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN1449160) Images
Sequence CPSTSRpSLY SSS
Specificity This antibody reacts with vimentin phosphorylated at Ser50.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Protein G agarose
Immunogen KLH-PV50 peptide
Clone TM50
Isotype IgG2a

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN1449160) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Components of intermediate filaments provide information on the origin of vertebrate cells. Antibody to vimentin can be used as to identify the vimentin subclass of intermediate filaments. Vimentin is a ~58 kDa, widely expressed protein that thought to be involved mainly in structural processes, such as wound healing. Scientists have also recently determined that activated human macrophages secrete vimentin into the extracellular space, and overproduction of vimentin has been associated with cellular senescence.Synonyms: VIM
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling ProductDetails: References for anti-Vimentin antibody (ABIN1449160) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details ProductDetails: References for anti-Vimentin antibody (ABIN1449160) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-Vimentin antibody (ABIN1449160)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Oguri, Inoko, Shima, Izawa, Arimura, Yamaguchi, Inagaki, Kaibuchi, Kikuchi, Inagaki: "Vimentin-Ser82 as a memory phosphorylation site in astrocytes." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 5, pp. 531-40, 2006

Utsugi, Minemura, Hirata, Abe, Watanabe, Ohya: "Movement of yeast 1,3-beta-glucan synthase is essential for uniform cell wall synthesis." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 7, Issue 1, pp. 1-9, 2002

Yanai, Saito, Kakinuma, Kon, Hirota, Taniguchi-Yanai, Nishijo, Shigematsu, Horiguchi, Kasuya, Sugiyama, Yagami, Murakami, Fukamizu: "Renin-dependent cardiovascular functions and renin-independent blood-brain barrier functions revealed by renin-deficient mice." in: The Journal of biological chemistry, Vol. 275, Issue 1, pp. 5-8, 2000

Nakamura, Hashimoto, Amano, Nagata, Matsumoto, Goto, Fukusho, Mori, Kashiwagi, Kudo, Inagaki, Takeda: "Localized phosphorylation of vimentin by rho-kinase in neuroblastoma N2a cells." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 5, Issue 10, pp. 823-37, 2000

Goto, Kosako, Tanabe, Yanagida, Sakurai, Amano, Kaibuchi, Inagaki: "Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis." in: The Journal of biological chemistry, Vol. 273, Issue 19, pp. 11728-36, 1998

Krueger, Daaka, Pitcher, Lefkowitz: "The role of sequestration in G protein-coupled receptor resensitization. Regulation of beta2-adrenergic receptor dephosphorylation by vesicular acidification." in: The Journal of biological chemistry, Vol. 272, Issue 1, pp. 5-8, 1997

Scholey: "Kinesin-II, a membrane traffic motor in axons, axonemes, and spindles." in: The Journal of cell biology, Vol. 133, Issue 1, pp. 1-4, 1996

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN1449160) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Vimentin (VIM) (pSer50) antibody (ABIN1449160) anti-Vimentin (VIM) (pSer50) antibody
Western Blotting (WB) image for anti-Vimentin (VIM) (pSer50) antibody (ABIN1449160) anti-Vimentin (VIM) (pSer50) antibody (Image 2)