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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||445 Antibodies||124 Antibodies||133 Antibodies|
|Transfected Cell Culture (TCC)||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Luminex Assay (LMNX)||8 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Immunoprecipitation (IP)||42 Antibodies||8 Antibodies||16 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||133 Antibodies||46 Antibodies||36 Antibodies|
|Immunohistochemistry (IHC)||223 Antibodies||56 Antibodies||63 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||40 Antibodies|
|Antigen||Vimentin (VIM) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Vimentin antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|11 references available|
|Supplier||Log in to see|
Product Details anti-Vimentin AntibodyTarget Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1109485) Images
|Specificity||This antibody reacts specifically with the phosphorylated MPV55 peptide but not the non-phosphorylated peptide. This antibody detects vimentin phosphorylated by cdc2 kinase and does not detect non-phosphorylated vimentin or phosphorylated vimentin by cAMP-dependent kinase, protein kinase C, or Ca2+-calmodulin-dependent protein kinase II on Western blotting.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, mouse, rat|
|Purification||Protein A agarose|
|Immunogen||Synthetic MPV55 phosphopeptide corresponding to mouse phosphorylated vimentin Ser55.|
Target Details VimentinProduct Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN1109485) Images back to top
|Alternative Name||Vimentin (VIM Antibody Abstract)|
|Background||Vimentin is an intermediate filament protein distributed widely in the cytoplasm and is phosphorylated by several protein kinase in vitro. Ser55 residues on vimentin were reported to be one of the phosphorylation sites of vimentin at metaphase and were the phosphorylation sites for cdc2 kinase but not for cAMP-dependent protein kinase, protein kinase C, and Ca2+-calmodulin-dependent protein kinase II in vitro. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and de-phosphorylated in cytokinesis. The use of this antibody that specifically reacts with the phosphorylation site of vimentin Ser55 by cdc2 kinase enables estimation of a particular cdc2 kinase function.Synonyms: VIM|
|Research Area||Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers|
|Pathways||TCR Signaling, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN1109485) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN1109485) Images back to top
|Buffer||PBS containing 50 % glycerol, pH 7.2. No preservative is contained.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store (in aliquots) at -20 °C.|
References for anti-Vimentin Antibody (ABIN1109485)Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Tatsumi, Sugimoto, Yugawa et al.: "Deregulation of Cdt1 induces chromosomal damage without rereplication and leads to chromosomal instability." in: Journal of cell science, Vol. 119, Issue Pt 15, pp. 3128-40, 2006 (PubMed).
Oguri, Inoko, Shima et al.: "Vimentin-Ser82 as a memory phosphorylation site in astrocytes." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 5, pp. 531-40, 2006 (PubMed).
Shiromizu, Goto, Tomono et al.: "Regulation of mitotic function of Chk1 through phosphorylation at novel sites by cyclin-dependent kinase 1 (Cdk1)." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 5, pp. 477-85, 2006 (PubMed).
Kohn, Aladjem, Weinstein et al.: "Molecular interaction maps of bioregulatory networks: a general rubric for systems biology." in: Molecular biology of the cell, Vol. 17, Issue 1, pp. 1-13, 2005 (PubMed).
Tsai, Chen, Kriegstein et al.: "LIS1 RNA interference blocks neural stem cell division, morphogenesis, and motility at multiple stages." in: The Journal of cell biology, Vol. 170, Issue 6, pp. 935-45, 2005 (PubMed).
Englund, Fink, Lau et al.: "Pax6, Tbr2, and Tbr1 are expressed sequentially by radial glia, intermediate progenitor cells, and postmitotic neurons in developing neocortex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 1, pp. 247-51, 2005 (PubMed).
Miyata, Kawaguchi, Saito et al.: "Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells." in: Development (Cambridge, England), Vol. 131, Issue 13, pp. 3133-45, 2004 (PubMed).
Nakamura, Hashimoto, Amano et al.: "Localized phosphorylation of vimentin by rho-kinase in neuroblastoma N2a cells." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 5, Issue 10, pp. 823-37, 2000 (PubMed).
Inada, Togashi, Nakamura et al.: "Balance between activities of Rho kinase and type 1 protein phosphatase modulates turnover of phosphorylation and dynamics of desmin/vimentin filaments." in: The Journal of biological chemistry, Vol. 274, Issue 49, pp. 34932-9, 2000 (PubMed).
Goto, Kosako, Tanabe et al.: "Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis." in: The Journal of biological chemistry, Vol. 273, Issue 19, pp. 11728-36, 1998 (PubMed).
Scholey: "Kinesin-II, a membrane traffic motor in axons, axonemes, and spindles." in: The Journal of cell biology, Vol. 133, Issue 1, pp. 1-4, 1996 (PubMed).
ImagesProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1109485) back to top