You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Sheep (Ovine) Vimentin antibody for Immunofluorescence

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Chicken, Human, Potoroo, Rat (Rattus), Sheep (Ovine)
838
363
311
219
137
131
114
83
79
49
48
41
41
36
30
10
7
6
5
5
4
3
2
2
2
1
1
1
1
1
Host
Mouse
518
295
29
14
12
8
7
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
27
27
23
20
15
14
12
11
11
11
11
11
11
11
11
9
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
665
289
283
264
238
220
216
106
82
40
15
14
13
10
8
8
5
3
3
3
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN112184
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4365312 CyTOF FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see V9 1
1 ABIN3026897 FACS IF IHC (p) Mouse IgM, kappa Log in to see LN-6
1 ABIN4365334 FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG1 Log in to see V9
1 ABIN4365357 FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG1 Log in to see V9
1 ABIN4365455 FACS IF IHC (p) Mouse IgM Log in to see LN-6
1 ABIN4365454 FACS IF IHC (p) Mouse IgM Log in to see LN-6
1 ABIN4365456 FACS IF IHC (p) Mouse IgM Log in to see LN-6
1 ABIN4365399 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG1 Log in to see V9
1 ABIN4365405 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 Log in to see V9
1 ABIN4365329 FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgG1 Log in to see V9
1 ABIN4365375 FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG1 Log in to see V9
1 ABIN4365387 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 Log in to see V9
1 ABIN4365393 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 Log in to see V9
1 ABIN4365367 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 Log in to see V9
1 ABIN4365463 CyTOF FACS IF IHC (p) Mouse IgM Log in to see LN-6
1 ABIN4365464 CyTOF FACS IF IHC (p) Mouse IgM Log in to see LN-6
1 ABIN1947912 IF IHC (p) Rabbit IgG AA 416-466 Log in to see Polyclonal
1 ABIN1947911 IF IHC (p) PLA WB Rabbit IgG AA 416-466 Log in to see Polyclonal
1 ABIN3026899 FACS IF IHC (p) Mouse IgM, kappa Log in to see LN-6

Top referenced anti-Vimentin antibody for Immunofluorescence

Similar anti-Vimentin Antibodies

Application / Reactivity Sheep (Ovine) Rat (Rattus)
Cytometry by Time of Flight (CyTOF) 3 Antibodies 5 Antibodies
Flow Cytometry (FACS) 39 Antibodies 91 Antibodies
Immunocytochemistry (ICC) 14 Antibodies 136 Antibodies
Immunofluorescence (IF) 20 Antibodies 149 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 100 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 13 Antibodies 52 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 41 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 19 Antibodies
ELISA 45 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Human, Potoroo, Rat (Rattus), Sheep (Ovine)
(838), (363), (311), (219), (137), (131), (114), (83), (79), (49), (48), (41), (41), (36), (30), (10), (7), (6), (5), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(518), (295), (29), (14), (12), (8), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(27), (27), (23), (20), (15), (14), (12), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(665), (289), (283), (264), (238), (220), (216), (106), (82), (40), (15), (14), (13), (10), (8), (8), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN112184) Images
Specificity The antibody clone V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Purification Purified
Immunogen V9 is an antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from Porcine lens.
Clone V9
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN112184) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.Synonyms: VIM
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN112184) Images back to top
Application Notes This antibody is suitable for Immunoblotting, Immunocytochemistry,Immunohistochemistry on frozen sections and paraffin-embedded tissues and Flowcytometry. Recommended dilutions: Flow cytometry: 1/25-1/200. Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent: 1/25-1/200. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer ( pH 6.0) is required. Immunoblotting applications: 1/100-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN112184) Images back to top
Concentration 1.0 mg/mL
Buffer PBS with 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Do not freeze working dilutions.
Storage 4 °C/-20 °C
Storage Comment Store the original vial at 2-8 °C. After reconstitution, aliquot and store stock solution or aliquots thereof at -20 °C.

References for anti-Vimentin Antibody (ABIN112184)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Corver, Koopman, van der Aa, Regensburg, Fleuren, Cornelisse: "Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer." in: Cytometry, Vol. 39, Issue 2, pp. 96-107, 2000

Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, 1987

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, 1987

Tölle, Weber, Osborn: "Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments." in: European journal of cell biology, Vol. 38, Issue 2, pp. 234-44, 1985

Osborn, Debus, Weber: "Monoclonal antibodies specific for vimentin." in: European journal of cell biology, Vol. 34, Issue 1, pp. 137-43, 1984