anti-Zebrafish Vimentin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Dog (Canine), Chicken, Goat, Human, Monkey, Mouse (Murine), Rat (Rattus), Pig (Porcine), Zebrafish
923
400
350
220
138
131
120
83
80
52
50
43
41
37
30
12
8
6
5
5
4
3
3
2
2
1
1
1
1
1
Host
Mouse
570
318
30
14
12
9
9
6
Clonality
Monoclonal
Conjugate
This Vimentin antibody is conjugated to FITC
29
28
25
23
17
16
13
13
13
13
13
13
13
13
12
10
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
735
341
327
305
253
248
248
142
87
40
15
14
13
10
8
7
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN1042493
$ 420.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1042494 ICC IHC (fro) FACS IHC (p) WB HRP Mouse IgG1 Log in to see RV202 5
1 ABIN4365315 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202
1 ABIN5618631 FACS IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202

General

Antigen Vimentin (VIM) Antibodies
Reactivity Dog (Canine), Chicken, Goat, Human, Monkey, Mouse (Murine), Rat (Rattus), Pig (Porcine), Zebrafish
(923), (400), (350), (220), (138), (131), (120), (83), (80), (52), (50), (43), (41), (37), (30), (12), (8), (6), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(570), (318), (30), (14), (12), (9), (9), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is conjugated to FITC
(29), (28), (25), (23), (17), (16), (13), (13), (13), (13), (13), (13), (13), (13), (12), (10), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(735), (341), (327), (305), (253), (248), (248), (142), (87), (40), (15), (14), (13), (10), (8), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN1042493) Images
Specificity RV202-FITC reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202-FITC is suitable for immunocytochemistry on cell cultures and cytospins, immuno-histochemistry on frozen tissues, as well as for flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:10.
Characteristics Cytoskeletal,Tumour marker
Purification Purified
Clone RV202
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN1042493) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
Research Area Cancer, Cytoskeleton, Immunology, Neurology, Stem Cells, Lineage Markers, Neural Stem Cell marker, Cell/Tissue Markers
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling ProductDetails: References for anti-Vimentin antibody (ABIN1042493) Images back to top
Application Notes RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
Comment

RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details ProductDetails: References for anti-Vimentin antibody (ABIN1042493) Images back to top
Format Liquid
Buffer PBS containing 0,1% BSA, 0.09% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

ProductDetails: References for anti-Vimentin antibody (ABIN1042493)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, 1988

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987