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anti-Human ZAP70 antibody for Intracellular Staining

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • 70kDa
  • AI327364
  • MGC80670
  • MGC88962
  • mrtle
  • mur
  • SRK
  • Srk
  • STCD
  • STD
  • TZK
  • ZAP-70
  • zap70
  • ZAP70
Epitope
pTyr319
64
51
46
46
25
22
21
21
20
15
15
15
14
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
530
186
121
4
4
3
2
2
2
Host
Mouse
298
212
15
8
3
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is conjugated to PerCP-Cy5.5
31
24
18
16
10
8
8
7
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
Application
Intracellular Staining (ICS)
297
163
155
148
121
68
54
44
24
7
5
4
4
4
3
2
Supplier
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Catalog No. ABIN1176924
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN1177223 ICS PE Mouse IgG1, kappa pTyr292 Log in to see J34-602 3
0.0008709465 ABIN1177225 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr292 Log in to see J34-602 3
0.0008709465 ABIN1177224 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr292 Log in to see J34-602 3
0.0008709465 ABIN281402 FACS IP ICS WB Mouse IgG1 kappa Log in to see 1E7-2

Top referenced anti-ZAP70 antibody for Intracellular Staining

Similar anti-ZAP70 Antibodies

Application / Reactivity Human
ELISA 160 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
FLISA 2 Antibodies
Flow Cytometry (FACS) 150 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 69 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies
Immunohistochemistry (IHC) 156 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 122 Antibodies
Immunoprecipitation (IP) 57 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Western Blotting (WB) 295 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope pTyr319
(64), (51), (46), (46), (25), (22), (21), (21), (20), (15), (15), (15), (14), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(530), (186), (121), (4), (4), (3), (2), (2), (2)
Host Mouse
(298), (212), (15), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is conjugated to PerCP-Cy5.5
(31), (24), (18), (16), (10), (8), (8), (7), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Intracellular Staining (ICS)
(297), (163), (155), (148), (121), (68), (54), (44), (24), (7), (5), (4), (4), (4), (3), (2)
Pubmed 3 references available
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN1176924) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human phosphorylated ZAP70 Peptide
Clone 17A-P
Isotype IgG1

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 Antibody (ABIN1176924) Images back to top
Antigen
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background ZAP70 is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both ZAP70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. TCR stimulation leads to autophosphorylation of ZAP70 at Tyr-315 amd Tyr-319, and mutation of the Tyr-319 site dramatically impairs TCR signaling. In addition, TCR-mediated Lck activity leads to phosphorylation of ZAP70 on Tyr-493 in the regulatory loop of the kinase domain leading to upregulation of ZAP70 kinase activity. The significance of ZAP70 activation in mediating TCR signal transduction has been confirmed by showing that ZAP70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP70 kinase domain which affect the stability of the protein and TCR signaling. Clone 17A/P-ZAP70 recognizes the phosphorylated form of ZAP70 (Y319). It also cross-reacts with SYK (Y352) due to homology of the phosphorylation site with ZAP70 (Y319). The PE-conjugated format has been evaluated using human and mouse model systems. The unconjugated form of the antibody (Cat. No. 612574) has also been shown to work in western blot analysis on human, mouse, and rat cells.
Synonyms: ZAP-70, SRK, STD, TZK, Zeta-chain associated protein kinase, 70kD
Research Area Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 Antibody (ABIN1176924) Images back to top
Sample Volume 5 μL
Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 Antibody (ABIN1176924) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed.

References for anti-ZAP70 Antibody (ABIN1176924)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Product cited in:

Di Bartolo, Mège, Germain, Pelosi, Dufour, Michel, Magistrelli, Isacchi, Acuto: "Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling." in: The Journal of biological chemistry, Vol. 274, Issue 10, pp. 6285-94, 1999

Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, 1994

Chan, Kadlecek, Elder, Filipovich, Kuo, Iwashima, Parslow, Weiss: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994