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anti-Human ZAP70 antibody for Intracellular Staining

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta-chain (TCR) associated protein kinase 70kDa
  • tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta-chain (TCR) associated protein kinase 70
  • zap70
  • ZAP70
  • Smp_149470
  • Zap70
Epitope
pTyr292
65
53
50
45
27
23
22
22
20
15
15
15
14
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
546
189
125
4
4
3
2
2
2
Host
Mouse
311
215
16
8
3
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is conjugated to Alexa Fluor 647
30
23
18
17
11
8
7
7
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
1
Application
Intracellular Staining (ICS)
300
167
154
146
122
67
52
44
21
7
5
5
4
4
3
3
2
Options
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Catalog No. ABIN1177224
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177225 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr292 Log in to see J34-602 3
1 ABIN1177223 ICS PE Mouse IgG1, kappa pTyr292 Log in to see J34-602 3
1 ABIN1176924 ICS PerCP-Cy5.5 Mouse IgG1 pTyr319 Log in to see 17A-P 3

Top referenced anti-ZAP70 antibody for Intracellular Staining

Similar anti-ZAP70 Antibodies

Application / Reactivity Human
Cytometry by Time of Flight (CyTOF) 5 Antibodies
ELISA 163 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
FLISA 2 Antibodies
Flow Cytometry (FACS) 146 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 66 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 52 Antibodies
Immunohistochemistry (IHC) 154 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 122 Antibodies
Immunoprecipitation (IP) 44 Antibodies
Intracellular Staining (ICS) 4 Antibodies
Simple Western (SimWes) 5 Antibodies
Western Blotting (WB) 295 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope pTyr292
(65), (53), (50), (45), (27), (23), (22), (22), (20), (15), (15), (15), (14), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(546), (189), (125), (4), (4), (3), (2), (2), (2)
Host Mouse
(311), (215), (16), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is conjugated to Alexa Fluor 647
(30), (23), (18), (17), (11), (8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1)
Application Intracellular Staining (ICS)
(300), (167), (154), (146), (122), (67), (52), (44), (21), (7), (5), (5), (4), (4), (3), (3), (2)
Pubmed 3 references available
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN1177224) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human ZAP70
Clone J34-602
Isotype IgG1, kappa

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 Antibody (ABIN1177224) Images back to top
Antigen
Alternative Name Zap70 (ZAP70 Antibody Abstract)
Background The 70-kDa zeta chain-associated protein (ZAP70) is a Syk-family protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. TCR-mediated Lck activity leads to phosphorylation of ZAP70 on Tyrosine 493 in the regulatory loop of the PTK domain leading to upregulation of ZAP70 kinase activity. Tyrosine 292 (Y292), in the linker region between the SH2 and PTK domains, is autophosphorylated by the activated PTK domain. By binding with c-Cbl, the phosphorylated Y292 can down-regulate TCR signaling. The J34-602 antibody recognizes ZAP70 phosphorylated at Y292.
Synonyms: ZAP-70 (pY292)
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 Antibody (ABIN1177224) Images back to top
Application Notes This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer). Any of the three BD Phosflow™ permeabilization buffers may be used.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 Antibody (ABIN1177224) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-ZAP70 Antibody (ABIN1177224)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Product cited in:

Magnan, Di Bartolo, Mura, Boyer, Richelme, Lin, Roure, Gillet, Arrieumerlou, Acuto, Malissen, Malissen: "T cell development and T cell responses in mice with mutations affecting tyrosines 292 or 315 of the ZAP-70 protein tyrosine kinase." in: The Journal of experimental medicine, Vol. 194, Issue 4, pp. 491-505, 2001

Rao, Lupher, Ota, Reedquist, Druker, Band: "The linker phosphorylation site Tyr292 mediates the negative regulatory effect of Cbl on ZAP-70 in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 9, pp. 4616-26, 2000

Kong, Dalton, Bubeck Wardenburg, Straus, Kurosaki, Chan: "Distinct tyrosine phosphorylation sites in ZAP-70 mediate activation and negative regulation of antigen receptor function." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 5026-35, 1996