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anti-Human ZAP70 antibody for Immunoprecipitation

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta-chain (TCR) associated protein kinase 70kDa
  • tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta-chain (TCR) associated protein kinase 70
  • zap70
  • ZAP70
  • Smp_149470
  • Zap70
Epitope
AA 468-619
62
50
48
37
28
23
22
14
13
12
10
10
10
8
8
8
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
530
215
140
14
11
10
8
8
6
6
4
3
1
Host
Mouse
301
207
15
8
3
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is un-conjugated
28
22
18
17
12
8
8
7
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
298
161
142
142
131
63
52
45
20
7
5
5
4
4
4
3
3
2
2
Options
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Catalog No. ABIN967848
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967849 IF IP IHC WB Mouse IgG2a AA 468-619 Log in to see 29-ZAP70 Kinase 5
1 ABIN205364 FACS IP WB Mouse IgG1 AA 282-307 Log in to see 1E7-2
1 ABIN2663949 FACS IP IHC PE Mouse IgG1 kappa, IgG1 Log in to see 1E7-2 4
1 ABIN438732 FACS IP Rabbit pTyr319 Log in to see Polyclonal
1 ABIN438734 IP Rabbit pTyr494 Log in to see Polyclonal
1 ABIN1875382 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1105091 IHC (p) IP ELISA WB Mouse IgG1, kappa Log in to see
1 ABIN5542964 IP WB Mouse IgG2b Log in to see 4B2-C1-H3
1 ABIN214953 IP ELISA WB Mouse IgG1, kappa AA 326-341 Log in to see
1 ABIN210659 ICC IP WB Mouse IgG1 AA 282-307 Log in to see
1 ABIN218909 IP Rabbit IgG Log in to see Polyclonal
1 ABIN2663950 FACS IP IHC PE Mouse IgG1 kappa, IgG1 Log in to see 1E7-2 4
1 ABIN221118 ICC FACS IF IP WB Rabbit IgG pTyr319 Log in to see Polyclonal
1 ABIN218908 FACS IP ELISA WB Rabbit IgG pTyr493 Log in to see Polyclonal
1 ABIN212704 FACS IP WB Mouse IgG2a Log in to see
1 ABIN2405264 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2880178 ICC IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN1724502 IHC (p) IP ELISA WB Mouse IgG1 kappa Log in to see
1 ABIN4366555 CyTOF FACS ICC IF IP WB Mouse IgG1 Log in to see 1E7-2
1 ABIN4366564 FACS ICC IF IP WB PerCP Mouse IgG1 Log in to see 1E7-2

Top referenced anti-ZAP70 antibody for Immunoprecipitation

Similar anti-ZAP70 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 21 Antibodies 46 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 22 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 16 Antibodies
Immunofluorescence (IF) 16 Antibodies 32 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies 52 Antibodies
Immunohistochemistry (IHC) 54 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 80 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope AA 468-619
(62), (50), (48), (37), (28), (23), (22), (14), (13), (12), (10), (10), (10), (8), (8), (8), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Human, Mouse (Murine), Rat (Rattus)
(530), (215), (140), (14), (11), (10), (8), (8), (6), (6), (4), (3), (1)
Host Mouse
(301), (207), (15), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is un-conjugated
(28), (22), (18), (17), (12), (8), (8), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(298), (161), (142), (142), (131), (63), (52), (45), (20), (7), (5), (5), (4), (4), (4), (3), (3), (2), (2)
Pubmed 5 references available
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN967848) Images
Cross-Reactivity Mouse (Murine), Rat (Rattus), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human ZAP-70 Kinase aa. 468-619
Clone 29-ZAP70 Kinase
Isotype IgG2a

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 Antibody (ABIN967848) Images back to top
Antigen
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background ZAP-70 is a protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 contains two SH2-like domains with the PTK domain being at the C-terminus. It appears that both ZAP-70 and Syk are recruited to the phosphorylated CD3 and zeta subunits after TCR stimulation. The src-family PTKs, lck, and fyn, seem to play a role upstream of ZAP-70 and Syk where they phosphorylate CD3 and zeta. Thus both Src- and ZAP-70/Syk-family PTKs are critical for efficient induction of tyrosine phosphoproteins in heterologous cells. The significance of ZAP-70 in mediating TCR signal transduction has been confirmed by showing that ZAP-70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP-70 kinase domain which affect the stability of the protein. The levels of lck and fyn appear to be normal in the same cells. Results of the ZAP-70 mutations in these patients include an unusual absence of peripheral CD8+ T cells and an abundance of CD4+ T cells in peripheral lymphoid organs. These results indicate that these two populations of T cells develop through utilization of different intracellular signaling pathways.
Molecular Weight 70 kDa
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 Antibody (ABIN967848) Images back to top
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 Antibody (ABIN967848) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-ZAP70 Antibody (ABIN967848)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Product cited in:

Michel, Attal-Bonnefoy, Mangino, Mise-Omata, Acuto: "CD28 as a molecular amplifier extending TCR ligation and signaling capabilities." in: Immunity, Vol. 15, Issue 6, pp. 935-45, 2001

Mora, Stanley, Armistead, Chan, Boothby: "Inefficient ZAP-70 phosphorylation and decreased thymic selection in vivo result from inhibition of NF-kappaB/Rel." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5628-35, 2001

Maccalli, Pisarra, Vegetti, Sensi, Parmiani, Anichini: "Differential loss of T cell signaling molecules in metastatic melanoma patients' T lymphocyte subsets expressing distinct TCR variable regions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6912-23, 2000

Background publications

Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, 1994

Chan, Kadlecek, Elder, Filipovich, Kuo, Iwashima, Parslow, Weiss: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN967848) back to top
Supplier Images
Western Blotting (WB) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (AA 468-619) (ABIN967848) Western blot analysis of ZAP-70 Kinase on a Jurkat cell lysate (Human T-cell leukemia...