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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||153 Antibodies|
|Immunochromatography (IC)||7 Antibodies|
|Immunocytochemistry (ICC)||24 Antibodies|
|Immunofluorescence (IF)||68 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||44 Antibodies|
|Immunohistochemistry (IHC)||152 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||121 Antibodies|
|Immunoprecipitation (IP)||56 Antibodies|
|Intracellular Staining (ICS)||5 Antibodies|
|Western Blotting (WB)||290 Antibodies|
|Antigen||zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies|
|Conjugate||This ZAP70 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-ZAP70 AntibodyTarget Details ZAP70 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of ZAP-70.|
|Cross-Reactivity (Details)||Does not cross-react with related proteins.|
|Immunogen||Purified recombinant human ZAP-70 protein fragments expressed in E.coli.|
Target Details ZAP70Product Details anti-ZAP70 Antibody Application Details Handling Images back to top
|Alternative Name||ZAP70 (ZAP70 Antibody Abstract)|
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility,adhesion and cytokine expression of mature T-cells,as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR),a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently,ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn,a large number of signaling molecules are recruited and ultimately lead to lymphokine production,T-cell proliferation and differentiation. Furthermore,ZAP70 controls cytoskeleton modifications,adhesion and mobility of T-lymphocytes,thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus,ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development,ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally,ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).
Subunit structure: Interacts with NFAM1. Interacts with adapter proteins SLA and SLA2,these interactions negatively regulates T-cell receptor signaling. Interacts with CBLB by similarity. Interacts with DEF6. Interacts (via SH2 domains) with RHOH,this interaction regulates ZAP70 subcellular localization by similarity. Interacts with FCRL3. Interacts with VAV1. Interacts with CD247/CD3Z,this interaction docks ZAP70 at the stimulated TCR. Interacts with CBL,this interaction promotes ubiquitination,internalization and subsequent degradation of CD247/CD3Z. Identified in a complex with CBL and UBE2L3.
Subcellular location: Cytoplasm. Cell membrane,Peripheral membrane protein. Note: In quiescent T-lymphocytes,it is cytoplasmic. Upon TCR activation,it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes By similarity.
Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. Contains 1 protein kinase domain. Contains 2 SH2 domains.
|Molecular Weight||70 kDa|
|Research Area||Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-ZAP70 Antibody Target Details ZAP70 Handling Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Images back to top
|Buffer||PBS (pH 7.4) containing with 0.02 % sodium azide and 50 % glycerol.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
ImagesProduct Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling back to top