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|Antigen||zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies|
|Epitope||AA 468-619 Alternatives|
|Reactivity||Chicken, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ZAP70 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-ZAP70 AntibodyTarget Details ZAP70 Application Details Handling References for anti-ZAP70 antibody (ABIN967848) Images
|Cross-Reactivity||Mouse (Murine), Rat (Rattus), Chicken|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human ZAP-70 Kinase aa. 468-619|
Target Details ZAP70Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 antibody (ABIN967848) Images back to top
|Alternative Name||ZAP-70 (ZAP70 Antibody Abstract)|
|Background||ZAP-70 is a protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 contains two SH2-like domains with the PTK domain being at the C-terminus. It appears that both ZAP-70 and Syk are recruited to the phosphorylated CD3 and zeta subunits after TCR stimulation. The src-family PTKs, lck, and fyn, seem to play a role upstream of ZAP-70 and Syk where they phosphorylate CD3 and zeta. Thus both Src- and ZAP-70/Syk-family PTKs are critical for efficient induction of tyrosine phosphoproteins in heterologous cells. The significance of ZAP-70 in mediating TCR signal transduction has been confirmed by showing that ZAP-70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP-70 kinase domain which affect the stability of the protein. The levels of lck and fyn appear to be normal in the same cells. Results of the ZAP-70 mutations in these patients include an unusual absence of peripheral CD8+ T cells and an abundance of CD4+ T cells in peripheral lymphoid organs. These results indicate that these two populations of T cells develop through utilization of different intracellular signaling pathways.|
|Molecular Weight||70 kDa|
|Research Area||Signaling, Tags/Labels, Immunology, Cancer|
Application DetailsProduct Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 antibody (ABIN967848) Images back to top
Related Products: ABIN968537, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 antibody (ABIN967848) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20°C.|
References for anti-ZAP70 antibody (ABIN967848)Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
|Product cited in:||
Michel, Attal-Bonnefoy, Mangino, Mise-Omata, Acuto: "CD28 as a molecular amplifier extending TCR ligation and signaling capabilities." in: Immunity, Vol. 15, Issue 6, pp. 935-45, 2001
Mora, Stanley, Armistead, Chan, Boothby: "Inefficient ZAP-70 phosphorylation and decreased thymic selection in vivo result from inhibition of NF-kappaB/Rel." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5628-35, 2001
Maccalli, Pisarra, Vegetti, Sensi, Parmiani, Anichini: "Differential loss of T cell signaling molecules in metastatic melanoma patients' T lymphocyte subsets expressing distinct TCR variable regions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6912-23, 2000
Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, 1994
Chan, Kadlecek, Elder, Filipovich, Kuo, Iwashima, Parslow, Weiss: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994
ImagesProduct Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling References for anti-ZAP70 antibody (ABIN967848) back to top