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anti-Human ZAP70 antibody for Immunoprecipitation

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta-chain (TCR) associated protein kinase 70kDa
  • tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta-chain (TCR) associated protein kinase 70
  • zap70
  • ZAP70
  • Smp_149470
  • Zap70
Reactivity
Human
526
214
139
12
10
10
9
7
5
4
4
3
1
Host
Mouse
297
207
15
8
3
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is un-conjugated
28
22
18
17
12
8
8
7
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
294
161
143
141
128
64
52
45
20
7
5
5
4
4
4
3
3
2
2
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Catalog No. ABIN2169577
$ 392.86
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967848 IF IP IHC WB Mouse IgG2a AA 468-619 Log in to see 29-ZAP70 Kinase 5
1 ABIN967849 IF IP IHC WB Mouse IgG2a AA 468-619 Log in to see 29-ZAP70 Kinase 5
1 ABIN205364 FACS IP WB Mouse IgG1 AA 282-307 Log in to see 1E7-2
1 ABIN2663949 FACS IP IHC PE Mouse IgG1 kappa, IgG1 Log in to see 1E7-2 4
1 ABIN1875382 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN438732 FACS IP Rabbit pTyr319 Log in to see Polyclonal
1 ABIN438734 IP Rabbit pTyr494 Log in to see Polyclonal
1 ABIN1105091 IHC (p) IP ELISA WB Mouse IgG1, kappa Log in to see
1 ABIN2663950 FACS IP IHC PE Mouse IgG1 kappa, IgG1 Log in to see 1E7-2 4
1 ABIN214953 IP ELISA WB Mouse IgG1, kappa AA 326-341 Log in to see
1 ABIN218909 IP Rabbit IgG Log in to see Polyclonal
1 ABIN210659 ICC IP WB Mouse IgG1 AA 282-307 Log in to see
1 ABIN218908 FACS IP ELISA WB Rabbit IgG pTyr493 Log in to see Polyclonal
1 ABIN221118 ICC FACS IF IP WB Rabbit IgG pTyr319 Log in to see Polyclonal
1 ABIN2405264 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1724502 IHC (p) IP ELISA WB Mouse IgG1 kappa Log in to see
1 ABIN212704 FACS IP WB Mouse IgG2a Log in to see
1 ABIN2880178 ICC IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN4366564 FACS ICC IF IP WB PerCP Mouse IgG1 Log in to see 1E7-2
1 ABIN4366555 CyTOF FACS ICC IF IP WB Mouse IgG1 Log in to see 1E7-2

Top referenced anti-ZAP70 antibody for Immunoprecipitation

Similar anti-ZAP70 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 5 Antibodies
ELISA 158 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
FLISA 2 Antibodies
Flow Cytometry (FACS) 141 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 63 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 52 Antibodies
Immunohistochemistry (IHC) 143 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 128 Antibodies
Immunoprecipitation (IP) 46 Antibodies
Intracellular Staining (ICS) 4 Antibodies
Simple Western (SimWes) 5 Antibodies
Western Blotting (WB) 291 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Reactivity Human
(526), (214), (139), (12), (10), (10), (9), (7), (5), (4), (4), (3), (1)
Host Mouse
(297), (207), (15), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is un-conjugated
(28), (22), (18), (17), (12), (8), (8), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(294), (161), (143), (141), (128), (64), (52), (45), (20), (7), (5), (5), (4), (4), (4), (3), (3), (2), (2)
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling Images
Specificity This antibody detects endogenous levels of ZAP-70.
Cross-Reactivity (Details) Does not cross-react with related proteins.
Purification Affinity purified
Immunogen Purified recombinant human ZAP-70 protein fragments expressed in E.coli.
Clone 4B2-C1-H3
Isotype IgG2b

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility,adhesion and cytokine expression of mature T-cells,as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR),a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently,ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn,a large number of signaling molecules are recruited and ultimately lead to lymphokine production,T-cell proliferation and differentiation. Furthermore,ZAP70 controls cytoskeleton modifications,adhesion and mobility of T-lymphocytes,thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus,ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development,ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally,ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).
Subunit structure: Interacts with NFAM1. Interacts with adapter proteins SLA and SLA2,these interactions negatively regulates T-cell receptor signaling. Interacts with CBLB by similarity. Interacts with DEF6. Interacts (via SH2 domains) with RHOH,this interaction regulates ZAP70 subcellular localization by similarity. Interacts with FCRL3. Interacts with VAV1. Interacts with CD247/CD3Z,this interaction docks ZAP70 at the stimulated TCR. Interacts with CBL,this interaction promotes ubiquitination,internalization and subsequent degradation of CD247/CD3Z. Identified in a complex with CBL and UBE2L3.
Subcellular location: Cytoplasm. Cell membrane,Peripheral membrane protein. Note: In quiescent T-lymphocytes,it is cytoplasmic. Upon TCR activation,it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes By similarity.
Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. Contains 1 protein kinase domain. Contains 2 SH2 domains.
Molecular Weight 70 kDa
Gene ID 7535
NCBI Accession NM_001079
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Images back to top
Format Liquid
Buffer PBS (pH 7.4) containing with 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling back to top
Supplier Images
Immunoprecipitation (IP) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (ABIN2169577) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody
Western Blotting (WB) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (ABIN2169577) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody (Image 2)