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anti-Human ZAP70 antibody for Western Blotting

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • 70kDa
  • AI327364
  • MGC80670
  • MGC88962
  • mrtle
  • mur
  • SRK
  • Srk
  • STCD
  • STD
  • TZK
  • ZAP-70
  • zap70
  • ZAP70
Epitope
AA 103-130, N-Term
64
51
47
46
25
22
21
21
19
15
15
15
14
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
529
186
121
4
4
3
2
2
2
Host
Rabbit
297
212
15
8
3
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
31
24
18
16
10
8
8
7
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
295
162
154
148
119
68
54
44
24
7
5
5
4
4
3
2
Supplier
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Catalog No. ABIN658067
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13357732 ABIN1532002 IHC ELISA WB Rabbit IgG pTyr493 Log in to see Polyclonal 2
0.13357732 ABIN658066 FACS IHC (p) WB Rabbit Ig AA 276-304, Center Log in to see Polyclonal 2
0.13357732 ABIN238530 FACS IF WB Mouse IgG1 C-Term Log in to see ZAP-03 4
0.13357732 ABIN969464 IHC ELISA WB Mouse IgG1 Log in to see 3D2A4 3
0.13357732 ABIN967848 IF IP IHC WB Mouse IgG2a AA 468-619 Log in to see 29-ZAP70 Kinase 5
0.13357732 ABIN967849 IF IP IHC WB Mouse IgG2a AA 468-619 Log in to see 29-ZAP70 Kinase 5
0.13357732 ABIN125756 ICC FACS WB Mouse IgG1 C-Term Log in to see ZAP-03 3
0.13357732 ABIN2465058 ELISA WB Goat Internal Region Log in to see Polyclonal
0.13357732 ABIN2788712 WB Rabbit N-Term Log in to see Polyclonal
0.13357732 ABIN1109533 EIA WB Goat Internal Region Log in to see Polyclonal
0.13357732 ABIN2169577 IP WB Mouse IgG2b Log in to see 4B2-C1-H3
0.13357732 ABIN967585 IHC (f) WB Mouse IgG1, kappa pTyr493 Log in to see 1a-Zap70 3
0.13357732 ABIN1532001 ELISA WB Rabbit IgG pTyr319 Log in to see Polyclonal
0.13357732 ABIN1531416 IHC ELISA WB Rabbit IgG pTyr315 Log in to see Polyclonal
0.13357732 ABIN1531415 ELISA WB Rabbit IgG pTyr292 Log in to see Polyclonal
0.13357732 ABIN498719 IF IHC (p) WB Rabbit pTyr493 Log in to see Polyclonal
0.13357732 ABIN492558 IHC (p) WB Mouse Log in to see 11H40
0.13357732 ABIN498718 IF IHC (p) WB Rabbit pTyr319 Log in to see Polyclonal
0.13357732 ABIN1532432 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13357732 ABIN1533039 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-ZAP70 antibody for Western Blotting

Similar anti-ZAP70 Antibodies

Application / Reactivity Human
ELISA 160 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
FLISA 2 Antibodies
Flow Cytometry (FACS) 150 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 69 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies
Immunohistochemistry (IHC) 156 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 122 Antibodies
Immunoprecipitation (IP) 57 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Western Blotting (WB) 295 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope AA 103-130, N-Term
(64), (51), (47), (46), (25), (22), (21), (21), (19), (15), (15), (15), (14), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(529), (186), (121), (4), (4), (3), (2), (2), (2)
Host Rabbit
(297), (212), (15), (8), (3)
Clonality (Clone)
Polyclonal   ( )
Conjugate This ZAP70 antibody is un-conjugated
(31), (24), (18), (16), (10), (8), (8), (7), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(295), (162), (154), (148), (119), (68), (54), (44), (24), (7), (5), (5), (4), (4), (3), (2)
Pubmed 2 references available
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN658067) Images
Specificity This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the N-terminal region of human ZAP70.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 AA from the N-terminal region of human ZAP70.
Clone RB34711
Isotype Ig

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 Antibody (ABIN658067) Images back to top
Antigen
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Synonyms: Tyrosine-protein kinase ZAP-70,ZAP70,SRK
Molecular Weight 69872 DA
Gene ID 7535
NCBI Accession NP_001070, NP_997402
UniProt P43403
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Chromatin
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 Antibody (ABIN658067) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 Antibody (ABIN658067) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment ZAP70 Antibody (N-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months

References for anti-ZAP70 Antibody (ABIN658067)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Background publications

Lin, Cheng, Huang, Lin, Yang: "Zap70 controls the interaction of talin with integrin to regulate the chemotactic directionality of T-cell migration." in: Molecular immunology, Vol. 47, Issue 11-12, pp. 2022-9, 2010

Liu, Purbhoo, Davis, Rudd: "SH2 domain containing leukocyte phosphoprotein of 76-kDa (SLP-76) feedback regulation of ZAP-70 microclustering." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 22, pp. 10166-71, 2010

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN658067) back to top
Supplier Images
Western Blotting (WB) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (AA 103-130) (ABIN658067) ZAP70 Antibody (N-term) (ABIN658067) western blot analysis in Jurkat cell line lysate...