anti-Rat (Rattus) ZAP70 antibody for ELISA

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta-chain (TCR) associated protein kinase 70kDa
  • tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta-chain (TCR) associated protein kinase 70
  • zap70
  • ZAP70
  • Smp_149470
  • Zap70
Epitope
pTyr493
79
52
49
37
28
24
23
15
13
12
10
10
10
8
8
8
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
580
218
142
14
11
10
9
8
6
6
4
3
1
Host
Rabbit
314
243
16
8
3
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
31
24
20
19
13
9
9
7
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
2
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
318
171
169
161
158
83
52
49
23
7
5
5
4
4
4
3
3
2
2
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Catalog No. ABIN1532002
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1532001 ELISA WB Rabbit IgG pTyr319 Log in to see Polyclonal
1 ABIN1533039 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1533040 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN2889082 ELISA IHC WB Rabbit IgG AA 286-335 Log in to see Polyclonal
1 ABIN2888558 ELISA WB Rabbit IgG pTyr319 Log in to see Polyclonal
1 ABIN2888559 IHC (p) ELISA WB Rabbit IgG pTyr493 Log in to see Polyclonal
1 ABIN2878972 IHC (p) ELISA Rabbit IgG AA 460-509 Log in to see Polyclonal
1 ABIN3182175 ELISA IF IHC WB Rabbit IgG pTyr319 Log in to see Polyclonal
1 ABIN3182176 ELISA IHC WB Rabbit IgG pTyr493 Log in to see Polyclonal
1 ABIN3187518 ELISA IF IHC Rabbit IgG Tyr341 Log in to see Polyclonal
1 ABIN3187520 ELISA IF IHC WB Rabbit IgG Tyr348 Log in to see Polyclonal
1 ABIN1740899 IHC (p) ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN2560808 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
1 ABIN1094788 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1094789 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN5545557 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN5545555 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1580282 ELISA WB Rabbit IgG pTyr319 Log in to see Polyclonal
1 ABIN1580283 IHC ELISA WB Rabbit IgG pTyr493 Log in to see Polyclonal
1 ABIN2154899 ELISA WB Rabbit IgG pTyr319 Log in to see Polyclonal

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope pTyr493
(79), (52), (49), (37), (28), (24), (23), (15), (13), (12), (10), (10), (10), (8), (8), (8), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(580), (218), (142), (14), (11), (10), (9), (8), (6), (6), (4), (3), (1)
Host Rabbit
(314), (243), (16), (8), (3)
Clonality
Polyclonal
Conjugate This ZAP70 antibody is un-conjugated
(31), (24), (20), (19), (13), (9), (9), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(318), (171), (169), (161), (158), (83), (52), (49), (23), (7), (5), (5), (4), (4), (4), (3), (3), (2), (2)
Pubmed 2 references available
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling ProductDetails: References for anti-ZAP70 antibody (ABIN1532002) Images
Specificity ZAP-70 (Phospho-Tyr493) Antibody detects endogenous levels of ZAP- 70 only when phosphorylated at Tyr493.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyr493.
Isotype IgG

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling ProductDetails: References for anti-ZAP70 antibody (ABIN1532002) Images back to top
Antigen
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background Molecular weight of target protein: 69 kDa
Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70, ZAP70, kinase ZAP70
Molecular Weight 69 kDa
Gene ID 7535
OMIM 176947
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling ProductDetails: References for anti-ZAP70 antibody (ABIN1532002) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:4000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.234569 (NCBI Gene Symbol: ZAP70)

Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details ProductDetails: References for anti-ZAP70 antibody (ABIN1532002) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

ProductDetails: References for anti-ZAP70 antibody (ABIN1532002)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Background publications

Hillier, Graves, Fulton, Fulton, Pepin, Minx, Wagner-McPherson, Layman, Wylie, Sekhon, Becker, Fewell, Delehaunty, Miner, Nash, Kremitzki, Oddy, Du, Sun, Bradshaw-Cordum, Ali, Carter, Cordes, Harris et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005

Chan, Iwashima, Turck, Weiss: "ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain." in: Cell, Vol. 71, Issue 4, pp. 649-62, 1992

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling ProductDetails: References for anti-ZAP70 antibody (ABIN1532002) back to top
Supplier Images
Western Blotting (WB) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody (ABIN1532002) Western blot analysis of extracts from Jurkat cells, using ZAP-70 (Phospho-Tyr493) An...
Immunohistochemistry (IHC) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody (ABIN1532002) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ZAP-...