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anti-Human AES antibody for Immunofluorescence

Recommended AES Antibody (supplied by: Log in to see )

Antigen
Amino-terminal Enhancer of Split (AES) Antibodies
  • AES
  • AES-1
  • aes-1
  • AES-2
  • aes-2
  • AL024115
  • esp1
  • Esp1
  • ESP1
  • Grg
  • GRG
  • grg
  • Grg-5
  • Grg5
  • GRG5
  • grg5
  • tle5
  • Tle5
  • TLE5
  • xgrg-5
  • zgc:64216
Epitope
N-Term
44
23
15
4
3
3
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine)
91
34
23
3
3
2
1
1
Host
Rabbit
75
12
7
Clonality
Polyclonal
Conjugate
This AES antibody is un-conjugated
4
4
4
4
4
4
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
81
42
13
8
6
5
3
3
1
1
1
1
Supplier
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Catalog No. ABIN499227
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32040194 ABIN499225 EIA IF WB Rabbit IgG N-Term, C-Term Log in to see Polyclonal 4
0.0010840179 ABIN302780 IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0010840179 ABIN303165 IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0010840179 ABIN2736244 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN3197865 IC IF WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN315483 ICC IF IHC (p) IHC WB Rabbit N-Term Log in to see Polyclonal
0.0010840179 ABIN121926 IF WB Rabbit Log in to see Polyclonal 3
0.0010840179 ABIN320509 IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010840179 ABIN3049185 ICC IF IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-AES antibody for Immunofluorescence

Similar anti-AES Antibodies

Application / Reactivity Mouse (Murine) Human
Agonist (Agon) 1 Antibodies
ELISA 14 Antibodies 42 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 11 Antibodies 14 Antibodies
Immunofluorescence (IF) 9 Antibodies 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Western Blotting (WB) 31 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen Amino-terminal Enhancer of Split (AES) Antibodies
Epitope N-Term
(44), (23), (15), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(91), (34), (23), (3), (3), (2), (1), (1)
Host Rabbit
(75), (12), (7)
Clonality
Polyclonal
Conjugate This AES antibody is un-conjugated
(4), (4), (4), (4), (4), (4), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(81), (42), (13), (8), (6), (5), (3), (3), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-AES Antibody

Target Details AES Application Details Handling References for anti-AES Antibody (ABIN499227) Images
Specificity AES antibody was raised against a 16 amino acid peptide from near the amino-terminus of human AES.
Purification Affinity chromatography purified via peptide column
Immunogen Human AES / Amino-terminal enhancer of split / GRG / ESP1 / TLE5 (N-Terminus) Peptide
Isotype IgG

Target Details AES

Product Details anti-AES Antibody Application Details Handling References for anti-AES Antibody (ABIN499227) Images back to top
Antigen
Alternative Name AES / ESP1 (AES Antibody Abstract)
Background Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (4).Synonyms: AES1, Amino-terminal enhancer of split, GRG protein, Gp130-associated protein GAM
Gene ID 166
NCBI Accession NP_001121
UniProt Q08117
Pathways WNT Signaling

Application Details

Product Details anti-AES Antibody Target Details AES Handling References for anti-AES Antibody (ABIN499227) Images back to top
Application Notes ELISA. Western Blot: AES antibody can be used for the detection of AES at 2 - 4 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AES Antibody Target Details AES Application Details References for anti-AES Antibody (ABIN499227) Images back to top
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-AES Antibody (ABIN499227)

Product Details anti-AES Antibody Target Details AES Application Details Handling Images back to top
Background publications

Martin, Vuori: "Regulation of Bcl-2 proteins during anoikis and amorphosis." in: Biochimica et biophysica acta, Vol. 1692, Issue 2-3, pp. 145-57, 2004

Jan, Matter, Pai, Chen, Pilch, Komatsu, Ong, Fukuda, Ruoslahti: "A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors." in: Cell, Vol. 116, Issue 5, pp. 751-62, 2004

Chen, Courey: "Groucho/TLE family proteins and transcriptional repression." in: Gene, Vol. 249, Issue 1-2, pp. 1-16, 2000

Miyasaka, Choudhury, Hou, Li: "Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein." in: European journal of biochemistry / FEBS, Vol. 216, Issue 1, pp. 343-52, 1993

Images

Product Details anti-AES Antibody Target Details AES Application Details Handling References for anti-AES Antibody (ABIN499227) back to top
Supplier Images
Immunofluorescence (IF) image for anti-AES antibody (Amino-terminal Enhancer of Split) (N-Term) (ABIN499227) Immunocytochemistry of AES in 293 cells with AES antibody at 10 μg/ml.
Immunofluorescence (IF) image for anti-AES antibody (Amino-terminal Enhancer of Split) (N-Term) (ABIN499227) Immunofluorescence of AES in 293 cells with AES Antibody at 20 μg/ml.
Western Blotting (WB) image for anti-AES antibody (Amino-terminal Enhancer of Split) (N-Term) (ABIN499227) Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ...