|Antigen||Axin 1 (AXIN1) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This AXIN1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|9 references available|
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Product Details anti-AXIN1 AntibodyTarget Details AXIN1 Application Details Handling References for anti-AXIN1 antibody (ABIN372934) Images
|Specificity||This antibody recognizes Axin 1 (AXIN1).|
Species reactivity (expected):Canine (Dog), Mouse and Rat.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide from C-terminus of Human AXIN1.|
Target Details AXIN1Product Details anti-AXIN1 Antibody Application Details Handling References for anti-AXIN1 antibody (ABIN372934) Images back to top
|Alternative Name||Axin-1 (AXIN1 Antibody Abstract)|
|Background||Axin-1 (Axis inhibition protein 1) and Axin-2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin-1 interacts with APC, GSK3-beta, Dvl and beta-Catenin and promotes the GSK3-beta-mediated phosphorylation and subsequent degradation of beta-Catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute Axin1 in mice (6). Axin-2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).Synonyms: AXIN, AXIN1, Axis inhibition protein 1|
|Pathways||WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling|
Application DetailsProduct Details anti-AXIN1 Antibody Target Details AXIN1 Handling References for anti-AXIN1 antibody (ABIN372934) Images back to top
ELISA: 1/28000. Immunohistochemistry on Paraffin Sections: 3.75 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-AXIN1 Antibody Target Details AXIN1 Application Details References for anti-AXIN1 antibody (ABIN372934) Images back to top
|Buffer||Tris-Saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-AXIN1 antibody (ABIN372934)Product Details anti-AXIN1 Antibody Target Details AXIN1 Application Details Handling Images back to top
Salahshor, Woodgett: "The links between axin and carcinogenesis." in: Journal of clinical pathology, Vol. 58, Issue 3, pp. 225-36, 2005
Zeng, Tamai, Doble, Li, Huang, Habas, Okamura, Woodgett, He: "A dual-kinase mechanism for Wnt co-receptor phosphorylation and activation." in: Nature, Vol. 438, Issue 7069, pp. 873-7, 2005
Chia, Costantini: "Mouse axin and axin2/conductin proteins are functionally equivalent in vivo." in: Molecular and cellular biology, Vol. 25, Issue 11, pp. 4371-6, 2005
Dalton: "Universities scramble to assess scope of falsified results." in: Nature, Vol. 438, Issue 7064, pp. 7, 2005
Luo, Lin: "Axin: a master scaffold for multiple signaling pathways." in: Neuro-Signals, Vol. 13, Issue 3, pp. 99-113, 2004
Jho, Zhang, Domon, Joo, Freund, Costantini: "Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway." in: Molecular and cellular biology, Vol. 22, Issue 4, pp. 1172-83, 2002
Lustig, Jerchow, Sachs, Weiler, Pietsch, Karsten, van de Wetering, Clevers, Schlag, Birchmeier, Behrens: "Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors." in: Molecular and cellular biology, Vol. 22, Issue 4, pp. 1184-93, 2002
Leung, Kolligs, Wu, Zhai, Kuick, Hanash, Cho, Fearon: "Activation of AXIN2 expression by beta-catenin-T cell factor. A feedback repressor pathway regulating Wnt signaling." in: The Journal of biological chemistry, Vol. 277, Issue 24, pp. 21657-65, 2002
Chen, Cizeau, Vande Velde, Park, Bozek, Bolton, Shi, Dubik, Greenberg: "Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 7-10, 1999
ImagesProduct Details anti-AXIN1 Antibody Target Details AXIN1 Application Details Handling References for anti-AXIN1 antibody (ABIN372934) back to top