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anti-Human AXIN1 antibody for ELISA

Recommended AXIN1 Antibody (supplied by: Log in to see )

Antigen
Axin 1 (AXIN1) Antibodies
  • AI316800
  • Axin
  • AXIN
  • axin
  • AXIN1
  • AXIN1-RGS
  • Fu
  • fused
  • Kb
  • Ki
  • kinky
  • knobbly
  • LOC100226467
  • PPP1R49
  • wu:fi22e03
  • wu:fj14c08
  • wu:fk11b03
  • xAxin
  • zgc:109872
Epitope
AA 643-741
49
19
16
15
15
13
10
4
3
3
2
2
2
2
1
1
1
Reactivity
Human
129
36
28
5
4
2
1
1
1
1
1
Host
Mouse
89
28
19
Clonality
Monoclonal
Conjugate
This AXIN1 antibody is un-conjugated
8
8
6
6
5
5
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
104
79
31
26
20
19
15
11
4
1
1
Supplier
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Catalog No. ABIN393560
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32693866 ABIN563582 IF PLA ELISA WB Mouse IgG2a kappa AA 643-740, partial Log in to see 2B11
0.0012117105 ABIN184720 IHC ELISA WB Goat C-Term Log in to see Polyclonal 1
0.0012117105 ABIN2465452 IHC ELISA Goat C-Term Log in to see Polyclonal
0.0012117105 ABIN351463 IHC (p) ELISA Goat C-Term Log in to see Polyclonal
0.0012117105 ABIN1974454 IHC ELISA WB PE Rabbit IgG C-Term, AA 717-746 Log in to see Polyclonal
0.0012117105 ABIN1974455 ELISA WB PE Rabbit IgG Internal Region, AA 329-359 Log in to see Polyclonal
0.0012117105 ABIN934064 IF ELISA WB Mouse IgG2b kappa AA 546-752 Log in to see AT1A4
0.0012117105 ABIN1969867 ELISA WB FITC Rabbit IgG Internal Region, AA 329-359 Log in to see Polyclonal
0.0012117105 ABIN1969866 IHC ELISA WB FITC Rabbit IgG C-Term, AA 717-746 Log in to see Polyclonal
0.0012117105 ABIN1961043 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 717-746 Log in to see Polyclonal
0.0012117105 ABIN1961044 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Internal Region, AA 329-359 Log in to see Polyclonal
0.0012117105 ABIN1963419 ELISA WB APC Rabbit IgG Internal Region, AA 329-359 Log in to see Polyclonal
0.0012117105 ABIN1963418 IHC ELISA WB APC Rabbit IgG C-Term, AA 717-746 Log in to see Polyclonal
0.0012117105 ABIN1966086 IHC ELISA WB Biotin Rabbit IgG C-Term, AA 717-746 Log in to see Polyclonal
0.0012117105 ABIN1966087 ELISA WB Biotin Rabbit IgG Internal Region, AA 329-359 Log in to see Polyclonal
0.0012117105 ABIN1732156 IHC (p) ELISA Goat C-Term Log in to see Polyclonal
0.0012117105 ABIN1972123 IHC ELISA WB HRP Rabbit IgG C-Term, AA 717-746 Log in to see Polyclonal
0.0012117105 ABIN1972124 ELISA WB HRP Rabbit IgG Internal Region, AA 329-359 Log in to see Polyclonal
0.0012117105 ABIN249485 IHC (p) IHC ELISA Goat IgG C-Term Log in to see Polyclonal
0.0012117105 ABIN1829231 IF ELISA WB Mouse IgG2a, kappa Log in to see 2B11

Top referenced anti-AXIN1 antibody for ELISA

Similar anti-AXIN1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 1 Antibodies
ELISA 79 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunofluorescence (IF) 26 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 31 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 20 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 100 Antibodies

General

Antigen Axin 1 (AXIN1) Antibodies
Epitope AA 643-741
(49), (19), (16), (15), (15), (13), (10), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human
(129), (36), (28), (5), (4), (2), (1), (1), (1), (1), (1)
Host Mouse
(89), (28), (19)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AXIN1 antibody is un-conjugated
(8), (8), (6), (6), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(104), (79), (31), (26), (20), (19), (15), (11), (4), (1), (1)
Pubmed 5 references available
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Product Details anti-AXIN1 Antibody

Target Details AXIN1 Application Details Handling References for anti-AXIN1 Antibody (ABIN393560) Images
Specificity AXIN1 (NP_003493, 643 a.a. ~ 741 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen AXIN1 (NP_003493, 643 a.a. ~ 741 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2B11
Isotype IgG2a kappa

Target Details AXIN1

Product Details anti-AXIN1 Antibody Application Details Handling References for anti-AXIN1 Antibody (ABIN393560) Images back to top
Antigen
Alternative Name AXIN1 (AXIN1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 95635 DA
Gene ID 12406
NCBI Accession NP_003493
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer
Pathways WNT Signaling

Application Details

Product Details anti-AXIN1 Antibody Target Details AXIN1 Handling References for anti-AXIN1 Antibody (ABIN393560) Images back to top
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin (cadherin-associated protein), beta 1, 88kDa, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene.

Restrictions For Research Use only

Handling

Product Details anti-AXIN1 Antibody Target Details AXIN1 Application Details References for anti-AXIN1 Antibody (ABIN393560) Images back to top
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-AXIN1 Antibody (ABIN393560)

Product Details anti-AXIN1 Antibody Target Details AXIN1 Application Details Handling Images back to top
Background publications

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010

Rose, Behm, Drgon, Johnson, Uhl: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010

Sue Ng, Mahmoudi, Li, Hatzis, Boersema, Mohammed, Heck, Clevers: "MAP3K1 functionally interacts with Axin1 in the canonical Wnt signalling pathway." in: Biological chemistry, Vol. 391, Issue 2-3, pp. 171-80, 2010

Yang, Xu, Han, Li, Liu, Zhao, Yang, Dong, Miao, Dai, Wang: "Axin downregulates TCF-4 transcription via beta-catenin, but not p53, and inhibits the proliferation and invasion of lung cancer cells." in: Molecular cancer, Vol. 9, pp. 25, 2010

Wooten, Iyer, Montefusco, Hedgepeth, Payne, Kapur, Housman, Mendelsohn, Huggins: "Application of gene network analysis techniques identifies AXIN1/PDIA2 and endoglin haplotypes associated with bicuspid aortic valve." in: PLoS ONE, Vol. 5, Issue 1, pp. e8830, 2010

Images

Product Details anti-AXIN1 Antibody Target Details AXIN1 Application Details Handling References for anti-AXIN1 Antibody (ABIN393560) back to top
Supplier Images
ELISA image for anti-AXIN1 antibody (Axin 1) (AA 643-741) (ABIN393560) Detection limit for recombinant GST tagged AXIN1 is approximately 0.3 ng/mL as a capt...