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|Application / Reactivity||Human|
|Dot Blot (DB)||1 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||10 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||31 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||16 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Western Blotting (WB)||83 Antibodies|
|Antigen||Axin 1 (AXIN1) Antibodies|
|Epitope||AA 643-741 Alternatives|
|Conjugate||This AXIN1 antibody is un-conjugated Alternatives|
ELISA, Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-AXIN1 AntibodyTarget Details AXIN1 Application Details Handling References for anti-AXIN1 Antibody (ABIN393560) Images
|Specificity||AXIN1 (NP_003493, 643 a.a. ~ 741 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Purification||This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||AXIN1 (NP_003493, 643 a.a. ~ 741 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
Target Details AXIN1Product Details anti-AXIN1 Antibody Application Details Handling References for anti-AXIN1 Antibody (ABIN393560) Images back to top
|Alternative Name||AXIN1 (AXIN1 Antibody Abstract)|
|Background||Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1|
|Molecular Weight||95635 DA|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer|
Application DetailsProduct Details anti-AXIN1 Antibody Target Details AXIN1 Handling References for anti-AXIN1 Antibody (ABIN393560) Images back to top
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
Background: This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin (cadherin-associated protein), beta 1, 88kDa, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene.
|Restrictions||For Research Use only|
HandlingProduct Details anti-AXIN1 Antibody Target Details AXIN1 Application Details References for anti-AXIN1 Antibody (ABIN393560) Images back to top
|Buffer||PBS with 0.09% (w/v) sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles|
|Expiry Date||6 months|
References for anti-AXIN1 Antibody (ABIN393560)Product Details anti-AXIN1 Antibody Target Details AXIN1 Application Details Handling Images back to top
Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).
Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).
Sue Ng, Mahmoudi, Li et al.: "MAP3K1 functionally interacts with Axin1 in the canonical Wnt signalling pathway." in: Biological chemistry, Vol. 391, Issue 2-3, pp. 171-80, 2010 (PubMed).
Yang, Xu, Han et al.: "Axin downregulates TCF-4 transcription via beta-catenin, but not p53, and inhibits the proliferation and invasion of lung cancer cells." in: Molecular cancer, Vol. 9, pp. 25, 2010 (PubMed).
Wooten, Iyer, Montefusco et al.: "Application of gene network analysis techniques identifies AXIN1/PDIA2 and endoglin haplotypes associated with bicuspid aortic valve." in: PLoS ONE, Vol. 5, Issue 1, pp. e8830, 2010 (PubMed).
ImagesProduct Details anti-AXIN1 Antibody Target Details AXIN1 Application Details Handling References for anti-AXIN1 Antibody (ABIN393560) back to top