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anti-Mouse (Murine) AXIN1 antibody for Enzyme Immunoassay

Recommended AXIN1 Antibody (supplied by: Log in to see )

Antigen
Axin 1 (AXIN1) Antibodies
  • AI316800
  • Axin
  • AXIN
  • axin
  • AXIN1
  • AXIN1-RGS
  • Fu
  • fused
  • Kb
  • Ki
  • kinky
  • knobbly
  • LOC100226467
  • PPP1R49
  • wu:fi22e03
  • wu:fj14c08
  • wu:fk11b03
  • xAxin
  • zgc:109872
Epitope
C-Term
47
16
15
15
13
10
4
4
3
3
2
2
2
2
2
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
111
34
26
4
4
2
1
1
1
1
1
Host
Goat
86
18
14
Clonality
Polyclonal
Conjugate
This AXIN1 antibody is un-conjugated
8
8
6
5
5
5
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
87
64
31
14
11
10
5
3
3
1
Supplier
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Catalog No. ABIN372934
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088824704 ABIN783749 EIA WB Rabbit C-Term Log in to see Polyclonal 2

Top referenced anti-AXIN1 antibody for Enzyme Immunoassay

Similar anti-AXIN1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Western Blotting (WB) 83 Antibodies 18 Antibodies 11 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies 3 Antibodies 6 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 31 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies 11 Antibodies
Immunofluorescence (IF) 10 Antibodies 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies

General

Antigen Axin 1 (AXIN1) Antibodies
Epitope C-Term
(47), (16), (15), (15), (13), (10), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(111), (34), (26), (4), (4), (2), (1), (1), (1), (1), (1)
Host Goat
(86), (18), (14)
Clonality
Polyclonal
Conjugate This AXIN1 antibody is un-conjugated
(8), (8), (6), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(87), (64), (31), (14), (11), (10), (5), (3), (3), (1)
Pubmed 9 references available
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Product Details anti-AXIN1 Antibody

Target Details AXIN1 Application Details Handling References for anti-AXIN1 Antibody (ABIN372934) Images
Sequence c-FEEKIIGKVE KVD
Specificity This antibody recognizes Axin 1 (AXIN1).
Cross-Reactivity (Details) Species reactivity (expected):Canine (Dog), Mouse and Rat.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography.
Immunogen Synthetic peptide from C-terminus of Human AXIN1.
Isotype IgG

Target Details AXIN1

Product Details anti-AXIN1 Antibody Application Details Handling References for anti-AXIN1 Antibody (ABIN372934) Images back to top
Antigen
Alternative Name Axin-1 (AXIN1 Antibody Abstract)
Background Axin-1 (Axis inhibition protein 1) and Axin-2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin-1 interacts with APC, GSK3-beta, Dvl and beta-Catenin and promotes the GSK3-beta-mediated phosphorylation and subsequent degradation of beta-Catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute Axin1 in mice (6). Axin-2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).Synonyms: AXIN, AXIN1, Axis inhibition protein 1
Gene ID 8312
NCBI Accession NP_003493
UniProt O15169
Pathways WNT Signaling

Application Details

Product Details anti-AXIN1 Antibody Target Details AXIN1 Handling References for anti-AXIN1 Antibody (ABIN372934) Images back to top
Application Notes ELISA: 1/28000. Immunohistochemistry on Paraffin Sections: 3.75 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AXIN1 Antibody Target Details AXIN1 Application Details References for anti-AXIN1 Antibody (ABIN372934) Images back to top
Concentration 0.5 mg/mL
Buffer Tris-Saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-AXIN1 Antibody (ABIN372934)

Product Details anti-AXIN1 Antibody Target Details AXIN1 Application Details Handling Images back to top
Background publications

Salahshor, Woodgett: "The links between axin and carcinogenesis." in: Journal of clinical pathology, Vol. 58, Issue 3, pp. 225-36, 2005

Zeng, Tamai, Doble, Li, Huang, Habas, Okamura, Woodgett, He: "A dual-kinase mechanism for Wnt co-receptor phosphorylation and activation." in: Nature, Vol. 438, Issue 7069, pp. 873-7, 2005

Chia, Costantini: "Mouse axin and axin2/conductin proteins are functionally equivalent in vivo." in: Molecular and cellular biology, Vol. 25, Issue 11, pp. 4371-6, 2005

Dalton: "Universities scramble to assess scope of falsified results." in: Nature, Vol. 438, Issue 7064, pp. 7, 2005

Luo, Lin: "Axin: a master scaffold for multiple signaling pathways." in: Neuro-Signals, Vol. 13, Issue 3, pp. 99-113, 2004

Jho, Zhang, Domon, Joo, Freund, Costantini: "Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway." in: Molecular and cellular biology, Vol. 22, Issue 4, pp. 1172-83, 2002

Lustig, Jerchow, Sachs, Weiler, Pietsch, Karsten, van de Wetering, Clevers, Schlag, Birchmeier, Behrens: "Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors." in: Molecular and cellular biology, Vol. 22, Issue 4, pp. 1184-93, 2002

Leung, Kolligs, Wu, Zhai, Kuick, Hanash, Cho, Fearon: "Activation of AXIN2 expression by beta-catenin-T cell factor. A feedback repressor pathway regulating Wnt signaling." in: The Journal of biological chemistry, Vol. 277, Issue 24, pp. 21657-65, 2002

Chen, Cizeau, Vande Velde, Park, Bozek, Bolton, Shi, Dubik, Greenberg: "Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 7-10, 1999

Images

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Supplier Images
 image for anti-AXIN1 antibody (Axin 1) (C-Term) (ABIN372934) anti-Axin 1 (AXIN1) (C-Term) antibody