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anti-Dog (Canine) CAMK2A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CAMK2A Antibody (supplied by: Log in to see )

Antigen
Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
  • camk2
  • camk2b
  • CAMKA
  • CaMKII
  • mKIAA0968
  • PK2CDD
  • PKCCD
  • R74975
  • zgc:112538
  • zgc:123320
Epitope
Internal Region
44
30
13
8
8
7
6
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
123
80
66
10
8
8
5
5
4
3
3
2
2
2
1
1
Host
Goat
115
54
17
Clonality
Polyclonal
Conjugate
This CAMK2A antibody is un-conjugated
6
6
6
6
6
5
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
175
86
55
43
28
26
22
8
7
2
2
2
2
1
Supplier
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Catalog No. ABIN452427
$ 379.50
Plus shipping costs $45.00

Top referenced anti-CAMK2A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-CAMK2A Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Western Blotting (WB) 119 Antibodies 65 Antibodies 81 Antibodies 9 Antibodies
Radioimmunoassay (RIA) 4 Antibodies 23 Antibodies 3 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 5 Antibodies 27 Antibodies 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies 15 Antibodies 29 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 13 Antibodies 29 Antibodies

General

Antigen Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
Epitope Internal Region
(44), (30), (13), (8), (8), (7), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(123), (80), (66), (10), (8), (8), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1)
Host Goat
(115), (54), (17)
Clonality
Polyclonal
Conjugate This CAMK2A antibody is un-conjugated
(6), (6), (6), (6), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(175), (86), (55), (43), (28), (26), (22), (8), (7), (2), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-CAMK2A Antibody

Target Details CAMK2A Application Details Handling References for anti-CAMK2A Antibody (ABIN452427) Images
Sequence c-PRTAQSEETR VWHR
Specificity This antibody is expected to recognize both reported isoforms (NP_057065.2, NP_741960.1).
Cross-Reactivity (Details) Species reactivity (expected):Rat, Canine.
Species reactivity (tested):Human, Mouse.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide with sequence from the internal region of the protein sequence

Target Details CAMK2A

Product Details anti-CAMK2A Antibody Application Details Handling References for anti-CAMK2A Antibody (ABIN452427) Images back to top
Antigen
Alternative Name CAMK2A (CAMK2A Antibody Abstract)
Background Ca2+/CalmodulinDependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulindependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning. CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1 % of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30 % of total protein in the post synaptic density (PSD) region. Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation.Synonyms: CAMKA, CaM kinase II subunit alpha, CaM-kinase II alpha chain, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain, KIAA0968
Gene ID 815
NCBI Accession NP_057065
UniProt Q9UQM7
Research Area Kinases/Phosphatases
Pathways WNT Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-CAMK2A Antibody Target Details CAMK2A Handling References for anti-CAMK2A Antibody (ABIN452427) Images back to top
Application Notes Peptide ELISA: 1/64000. Western blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details References for anti-CAMK2A Antibody (ABIN452427) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-CAMK2A Antibody (ABIN452427)

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Handling Images back to top
Background publications

Lee, Lin, Luo, Lee, Lee, Aird, Hwang, Yang et al.: "Tumor necrosis factor-alpha enhances neutrophil adhesiveness: induction of vascular cell adhesion molecule-1 via activation of Akt and CaM kinase II and modifications of histone acetyltransferase and ..." in: Molecular pharmacology, Vol. 73, Issue 5, pp. 1454-64, 2008

Images

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Handling References for anti-CAMK2A Antibody (ABIN452427) back to top
Supplier Images
 image for anti-CAMK2A antibody (Calcium/calmodulin-Dependent Protein Kinase II alpha) (Internal Region) (ABIN452427) anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (Internal Region) antibody
 image for anti-CAMK2A antibody (Calcium/calmodulin-Dependent Protein Kinase II alpha) (Internal Region) (ABIN452427) anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (Internal Region) antibody (Image 2)