anti-Rat (Rattus) CAMK2A antibody for Immunohistochemistry (Frozen Sections)

Recommended CAMK2A Antibody (supplied by: Log in to see )

Antigen
Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
  • camk2
  • camk2b
  • zgc:112538
  • zgc:123320
  • CAMKA
  • CaMKII
  • R74975
  • mKIAA0968
  • PK2CDD
  • PKCCD
  • calcium/calmodulin-dependent protein kinase II alpha
  • calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
  • CAMK2A
  • camk2a
  • Camk2a
Epitope
pThr286
38
30
12
10
9
7
6
6
5
5
4
4
3
3
3
2
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
145
99
91
28
10
9
6
5
4
2
2
2
2
2
1
1
1
1
1
1
Host
Mouse
118
53
18
Clonality
Monoclonal
Conjugate
This CAMK2A antibody is un-conjugated
6
6
6
6
6
5
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
179
87
58
54
43
26
23
6
5
3
2
2
2
1
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Catalog No. ABIN5550935
$ 375.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN227221 IHC (fro) WB Rabbit pThr286 Log in to see Polyclonal
1 ABIN227220 IHC (fro) WB Rabbit pThr305 Log in to see Polyclonal
1 ABIN5550934 EIA IF IHC (fro) IP RIA WB Mouse IgG1 Log in to see 6G9

General

Antigen Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
Epitope pThr286
(38), (30), (12), (10), (9), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(145), (99), (91), (28), (10), (9), (6), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(118), (53), (18)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CAMK2A antibody is un-conjugated
(6), (6), (6), (6), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(179), (87), (58), (54), (43), (26), (23), (6), (5), (3), (2), (2), (2), (1)
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Product Details anti-CAMK2A Antibody

Target Details CAMK2A Application Details Handling Images
Cross-Reactivity (Details) Species reactivity (tested):Rat, Human.
Characteristics Synonyms: CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-IIsubunit alpha, Calcium/calmodulin-dependent protein kinase type II subunit alpha
Purification Protein G Chromatography.
Immunogen Synthetic peptide
Clone 22B1
Isotype IgG1

Target Details CAMK2A

Product Details anti-CAMK2A Antibody Application Details Handling Images back to top
Antigen
Alternative Name CAMK2A (CAMK2A Antibody Abstract)
Background CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca 2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).Synonyms: CAMKA, CaM kinase II subunit alpha, CaM-kinase II alpha chain, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II subunit alpha, KIAA0968
Gene ID 25400
UniProt P11275
Research Area Kinases/Phosphatases
Pathways WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction

Application Details

Product Details anti-CAMK2A Antibody Target Details CAMK2A Handling Images back to top
Application Notes ELISA. Immunflourescence: 10 μg/mL. Immunoprecipitaion. ImmunohistochemistryWestern blot: 1 μg/mL, A band of approx. 50 kDa is detected.1 μg/mL of CAMKII was sufficient for detection of 0.2 μg CAMKII by colorimetric immunoblot. (Ref.13)
Restrictions For Research Use only

Handling

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS pH 7.4 containing 0.09 % Sodium Azide in 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months