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anti-Dog (Canine) CTNNB1 antibody for Western Blotting

Recommended CTNNB1 Antibody (supplied by: Log in to see )

Antigen
Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies
  • armadillo
  • beta-catenin
  • Bfc
  • Catnb
  • CHBCAT
  • CTNNB
  • ctnnb
  • CTNNB1
  • ctnnb1
  • id:ibd2058
  • Jup
  • LOC100217813
  • Mesc
  • MRD19
  • wu:fb73e10
  • wu:fi81c06
  • wu:fk25h01
Epitope
Exon 3
99
25
20
20
19
16
15
14
12
7
7
7
7
6
6
5
5
5
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine)
445
108
96
27
21
13
13
6
5
4
4
2
1
1
1
Host
Mouse
316
153
2
2
Clonality
Monoclonal
Conjugate
This CTNNB1 antibody is un-conjugated
15
12
9
6
6
6
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
402
153
116
99
83
66
65
32
16
12
8
4
3
3
2
1
1
1
Supplier
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Catalog No. ABIN126747
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN2792526 WB Rabbit N-Term Log in to see Polyclonal 1
0.12303223 ABIN2792527 ChIP WB Rabbit Middle Region Log in to see Polyclonal 1
0.12303223 ABIN2792303 IHC WB Rabbit C-Term Log in to see Polyclonal 1
0.12303223 ABIN126742 WB Mouse IgG1 pTyr654 Log in to see 1B11
0.12303223 ABIN2454138 FACS IF WB Mouse IgG1 Log in to see 12C1
0.12303223 ABIN2454136 FACS IF IHC WB Mouse IgG2a Log in to see 1E10
0.12303223 ABIN2454137 FACS WB Mouse IgG2a Log in to see 6E7
0.12303223 ABIN2454170 FACS IHC WB Mouse IgG2b Log in to see 9H2
0.12303223 ABIN2452237 WB Mouse IgG1 AA 531-781 Log in to see 2D11
0.12303223 ABIN2452306 WB Mouse IgG2a AA 531-781 Log in to see 2H3
0.12303223 ABIN2452315 IHC WB Mouse IgG2a AA 531-781 Log in to see 1F3
0.12303223 ABIN2463774 IHC ELISA WB Rabbit Log in to see Polyclonal
0.12303223 ABIN2792528 ChIP WB Rabbit C-Term Log in to see Polyclonal
0.0011806369 ABIN126741 WB Biotin Mouse IgG1 pTyr654 Log in to see 1B11
0.0011806369 ABIN126746 EIA IF IHC (p) IP WB Biotin Mouse IgG1 Exon 3 Log in to see 9G2
0.0011806369 ABIN926052 IHC WB Rabbit C-Term Log in to see Polyclonal
0.0011806369 ABIN2670095 FACS IHC WB Mouse IgG2b Log in to see OTI1G1
0.0011806369 ABIN2670097 FACS IF WB Mouse IgG2a Log in to see OTI2F6
0.0011806369 ABIN2670098 FACS IHC WB Mouse IgG1 Log in to see OTI3F9
0.0011806369 ABIN2670099 IF IHC WB Mouse IgG2b Log in to see OTI9C1

Similar anti-CTNNB1 Antibodies

Application / Reactivity Mouse (Murine) Human
Chromatin Immunoprecipitation (ChIP) 2 Antibodies 3 Antibodies
ELISA 25 Antibodies 145 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies 13 Antibodies
Flow Cytometry (FACS) 9 Antibodies 65 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 26 Antibodies
Immunohistochemistry (IHC) 35 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies
Immunoprecipitation (IP) 18 Antibodies
Western Blotting (WB) 100 Antibodies
ELISA (Capture) 1 Antibodies

General

Antigen Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies
Epitope Exon 3
(99), (25), (20), (20), (19), (16), (15), (14), (12), (7), (7), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine)
(445), (108), (96), (27), (21), (13), (13), (6), (5), (4), (4), (2), (1), (1), (1)
Host Mouse
(316), (153), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CTNNB1 antibody is un-conjugated
(15), (12), (9), (6), (6), (6), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(402), (153), (116), (99), (83), (66), (65), (32), (16), (12), (8), (4), (3), (3), (2), (1), (1), (1)
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Product Details anti-CTNNB1 Antibody

Target Details CTNNB1 Application Details Handling Images
Specificity This antibody specifically interacts with exon 3 (alpha-Catenin-binding site) of beta-Catenin.
Characteristics Synonyms: CTNNB1, CTNNB, beta-1 Catenin, beta catenin, beta-Catenin 1, Catenin-beta 1
Purification Size Exclusion Chromatography
Components incl. positive Control
Immunogen Recombinant β-Catenin
Clone 9G2
Isotype IgG1

Target Details CTNNB1

Product Details anti-CTNNB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Catenin beta-1 (CTNNB1 Antibody Abstract)
Background The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of β-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighbouring cells. Recent studies indicate that β-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. β-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that β-catenin plays a dual role not only in the maintainance and regulation of cell-cell interactions but also in the regulation of gene activity.Synonyms: Beta-catenin, CTNNB, CTNNB1
Molecular Weight 90 kDa
Gene ID 1499
UniProt P35222
Research Area Signaling
Pathways WNT Signaling

Application Details

Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Handling Images back to top
Application Notes ELISA: 0.05 μg/mL. Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Immunoprecipitation: 1-10 μg per 10^6 vanadate treated A431 cells. Immunocytochemistry: 0.1-1 μg/mL. Immunohistochemistry on Paraffin Sections. Included Positive Control: Cell lysate from untreated SW480 cells (for details seeProtocols).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive Control: Cell lysate from untreated SW480 cells. Formulation: Lyophilized Cell Lysate from Serum starved SW480 cells. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control Cell Lysate is recommended for immunoblot applications. Use 20µlmolecular weight marker per lane. Note: Use BSA based blot incubation buffers. Milk,Casein and Blotto might interfere with antibody - antigen interaction.
Restrictions For Research Use only

Handling

Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CTNNB1 antibody (Catenin (Cadherin-Associated Protein), beta 1, 88kDa) (Exon 3) (ABIN126747) anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (Exon 3) antibody