anti-Hamster CTNNB1 antibody for Immunocytochemistry

Recommended CTNNB1 Antibody (supplied by: Log in to see )

Antigen
Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies
  • Jup
  • LOC100217813
  • CTNNB1
  • CTNNB
  • Bfc
  • Catnb
  • Mesc
  • ctnnb
  • id:ibd2058
  • wu:fb73e10
  • wu:fi81c06
  • wu:fk25h01
  • ctnnb1
  • CHBCAT
  • MRD19
  • armadillo
  • beta-catenin
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin (cadherin associated protein), beta 1
  • catenin (cadherin-associated protein), beta 1
  • CTNNB1
  • LOC100217813
  • Ctnnb1
  • ctnnb1
  • ctnnb1-a
  • ctnnb1-b
Epitope
C-Term, AA 441-448
79
21
20
20
19
17
16
15
12
10
9
8
7
7
6
5
5
5
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Hamster
475
212
189
63
53
41
26
25
21
21
15
15
14
10
9
9
3
1
1
1
1
1
1
Host
Mouse
308
180
6
2
Clonality
Monoclonal
Conjugate
This CTNNB1 antibody is un-conjugated
14
11
8
7
6
6
4
4
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
438
154
127
123
111
97
68
30
16
13
8
8
4
3
3
2
2
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN457419
$ 288.75
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN574434 ICC FACS IHC (p) IP WB Mouse IgG1 Log in to see EM-22
1 ABIN211079 ICC IP WB Rabbit IgG AA 29-49 Log in to see Polyclonal
1 ABIN2619644 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 1-19 Log in to see Polyclonal
1 ABIN1723571 ICC FACS IHC (p) IP WB Mouse IgG1 Log in to see EM-22

General

Antigen Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies
Epitope C-Term, AA 441-448
(79), (21), (20), (20), (19), (17), (16), (15), (12), (10), (9), (8), (7), (7), (6), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Hamster
(475), (212), (189), (63), (53), (41), (26), (25), (21), (21), (15), (15), (14), (10), (9), (9), (3), (1), (1), (1), (1), (1), (1)
Host Mouse
(308), (180), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CTNNB1 antibody is un-conjugated
(14), (11), (8), (7), (6), (6), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(438), (154), (127), (123), (111), (97), (68), (30), (16), (13), (8), (8), (4), (3), (3), (2), (2), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-CTNNB1 Antibody

Target Details CTNNB1 Application Details Handling ProductDetails: References for anti-CTNNB1 antibody (ABIN457419) Images
Specificity The antibody EM-22 reacts with C-terminal part of beta-catenin, an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Recombinant human beta-catenin
Clone EM-22
Isotype IgG1

Target Details CTNNB1

Product Details anti-CTNNB1 Antibody Application Details Handling ProductDetails: References for anti-CTNNB1 antibody (ABIN457419) Images back to top
Antigen
Alternative Name CTNB1 (CTNNB1 Antibody Abstract)
Background Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumour cells. Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2

Application Details

Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Handling ProductDetails: References for anti-CTNNB1 antibody (ABIN457419) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details ProductDetails: References for anti-CTNNB1 antibody (ABIN457419) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

ProductDetails: References for anti-CTNNB1 antibody (ABIN457419)

Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Handling Images back to top
Background publications

Nowak, Madej, Dziegiel: "Expression of E-cadherin, beta-catenin and Ki-67 antigen and their reciprocal relationships in mammary adenocarcinomas in bitches." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45, Issue 3, pp. 233-8, 2007

Jung, Chung, Kim, Kim, Heo, Ahn, Hwang, Kim, Lee, Kim, Kim, Chang: "Epstein-Barr virus, beta-catenin, and E-cadherin in gastric carcinomas." in: Journal of Korean medical science, Vol. 22, Issue 5, pp. 855-61, 2007

Valenta, Lukas, Korinek: "HMG box transcription factor TCF-4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells." in: Nucleic acids research, Vol. 31, Issue 9, pp. 2369-80, 2003

Images

Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Handling ProductDetails: References for anti-CTNNB1 antibody (ABIN457419) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (AA 441-448), (C-Term) antibody (ABIN457419) Immunocytochemistry staining of beta-catenin in humancolon adenocarcinoma cell line H...
Western Blotting (WB) image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (AA 441-448), (C-Term) antibody (ABIN457419) Western blotting analysis of beta-catenin in murine 3T3 (A), C57 (B) and KW1 (C) cell...
Flow Cytometry (FACS) image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (AA 441-448), (C-Term) antibody (ABIN457419) Flow cytometry analysis (intracellular) of beta-catenin in human MCF-7 cell line by -...