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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||380 Antibodies||99 Antibodies||85 Antibodies|
|RNA Interference (RNAi)||2 Antibodies|
|Proximity Ligation Assay (PLA)||4 Antibodies|
|Luminex Assay (LMNX)||8 Antibodies|
|Immunoprecipitation (IP)||61 Antibodies||18 Antibodies||10 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||78 Antibodies||30 Antibodies||22 Antibodies|
|Immunohistochemistry (IHC)||116 Antibodies||36 Antibodies||36 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||10 Antibodies||4 Antibodies||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Antigen||Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies|
|Epitope||pSer33, pSer37, pThr41 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CTNNB1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-CTNNB1 AntibodyTarget Details CTNNB1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of p-beta-catenin protein only when phosphorylated at Ser33/S37/T41.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details CTNNB1Product Details anti-CTNNB1 Antibody Application Details Handling Images back to top
|Alternative Name||Catenin beta-1 (CTNNB1 Antibody Abstract)|
|Background||The catenins, alpha, beta and gamma, are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play critical roles in mediating cellular adhesion. beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. beta-catenin also forms complexes with the tumor suppressor protein APC. Amino acid alterations at residues around Ser 33, one of the targets for phosphorylation of glycogen synthase kinase-3beta, result in accumulation of the beta-catenin protein in the cytoplasm and nucleus. Pin1 is a novel regulator of beta-catenin signaling that directly binds a phosphorylated Ser-ro motif next to the APC-binding site in beta-catenin, inhibiting the interaction with APC and increasing beta-catenin translocation into the nucleus. Thus, Pin1 overexpression may contribute to the upregulation of beta-catenin in tumors such as breast cancer.Synonyms: Beta-catenin, CTNNB, CTNNB1|
|Molecular Weight||approx. 92 kDa|
Application DetailsProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Handling Images back to top
Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Handling back to top