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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Chromatin Immunoprecipitation (ChIP)||1 Antibodies||2 Antibodies|
|ELISA||23 Antibodies||24 Antibodies|
|Enzyme Immunoassay (EIA)||9 Antibodies||11 Antibodies|
|Flow Cytometry (FACS)||9 Antibodies||9 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies||9 Antibodies|
|Immunofluorescence (IF)||25 Antibodies||26 Antibodies|
|Immunohistochemistry (IHC)||35 Antibodies||34 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||22 Antibodies||30 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies|
|Western Blotting (WB)||84 Antibodies|
|Antigen||Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies|
|Epitope||AA 1-19, N-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CTNNB1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-CTNNB1 AntibodyTarget Details CTNNB1 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Catenin beta-1(CTNNB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: catenin(cadherin-associated protein), beta 1, 88 kDa
Protein Name: Catenin beta-1
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human beta Catenin(1-19aa MATQADLMELDMAMEPDRK), identical to the related rat and mouse sequences.|
Target Details CTNNB1Product Details anti-CTNNB1 Antibody Application Details Handling Images back to top
|Alternative Name||CTNNB1 (CTNNB1 Antibody Abstract)|
|Background||Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development(both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.|
Application DetailsProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Handling back to top