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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||6 Antibodies||2 Antibodies||2 Antibodies|
|Simple Western (SimWes)||1 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||417 Antibodies||189 Antibodies||165 Antibodies|
|RNA Interference (RNAi)||2 Antibodies|
|Proximity Ligation Assay (PLA)||4 Antibodies||1 Antibodies||1 Antibodies|
|Luminex Assay (LMNX)||8 Antibodies|
|Immunoprecipitation (IP)||67 Antibodies||52 Antibodies||35 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||118 Antibodies||79 Antibodies||63 Antibodies|
|Antigen||Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CTNNB1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-CTNNB1 AntibodyTarget Details CTNNB1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Catenin-beta protein only when phosphorylated at Ser33.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details CTNNB1Product Details anti-CTNNB1 Antibody Application Details Handling Images back to top
|Alternative Name||Catenin beta-1 (CTNNB1 Antibody Abstract)|
|Background||β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.Synonyms: Beta-catenin, CTNNB, CTNNB1|
|Molecular Weight||approx. 92 kDa|
|Pathways||WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2|
Application DetailsProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Handling Images back to top
Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Handling back to top