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|Antigen||Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) Antibodies|
|Epitope||C-Term, N-Term Alternatives|
|Conjugate||This DAAM1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-DAAM1 AntibodyTarget Details DAAM1 Application Details Handling References for anti-DAAM1 antibody (ABIN357856) Images
|Specificity||This antibody detects DAAM1 (C-term).|
|Purification||Protein A Chromatography followed by peptide affinity purification.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DAAM1.|
Target Details DAAM1Product Details anti-DAAM1 Antibody Application Details Handling References for anti-DAAM1 antibody (ABIN357856) Images back to top
|Alternative Name||DAAM1 (DAAM1 Antibody Abstract)|
|Background||Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. DAAM1 contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by DAAM1, which is thought to function as a scaffolding protein.Synonyms: DAAM-1, Disheveled-associated activator of morphogenesis 1, FLJ41657, KIAA0666|
|Molecular Weight||123473 Da|
|Gene ID||23002, 9606|
Application DetailsProduct Details anti-DAAM1 Antibody Target Details DAAM1 Handling References for anti-DAAM1 antibody (ABIN357856) Images back to top
ELISA: 1/1,000. Western Blot: 1/50-1/100. Immunohistochemistry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-DAAM1 Antibody Target Details DAAM1 Application Details References for anti-DAAM1 antibody (ABIN357856) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-DAAM1 antibody (ABIN357856)Product Details anti-DAAM1 Antibody Target Details DAAM1 Application Details Handling Images back to top
Liu, Sato, Khadka, Bharti, Diaz, Runnels, Habas: "Mechanism of activation of the Formin protein Daam1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 1, pp. 210-5, 2008
Yamashita, Higashi, Suetsugu, Sato, Ikeda, Shirakawa, Kita, Takenawa, Horiuchi, Fukai, Nureki: "Crystal structure of human DAAM1 formin homology 2 domain." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 12, Issue 11, pp. 1255-65, 2007
Lu, Meng, Poy, Maiti, Goode, Eck: "Structure of the FH2 domain of Daam1: implications for formin regulation of actin assembly." in: Journal of molecular biology, Vol. 369, Issue 5, pp. 1258-69, 2007
ImagesProduct Details anti-DAAM1 Antibody Target Details DAAM1 Application Details Handling References for anti-DAAM1 antibody (ABIN357856) back to top