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anti-Human DAAM1 antibody for Enzyme Immunoassay

Recommended DAAM1 Antibody (supplied by: Log in to see )

Antigen
Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) Antibodies
  • 1700066F09Rik
  • 2310028E21Rik
  • AI503486
  • daam-1
  • daam1
  • DAAM1
  • DKFZp468D0524
  • E130308H01
  • mKIAA0666
  • xdaam1
Epitope
C-Term, N-Term
34
11
8
3
3
3
2
2
2
2
1
1
Reactivity
Human
75
13
13
3
3
2
2
1
1
Host
Rabbit
69
6
Clonality
Polyclonal
Conjugate
This DAAM1 antibody is un-conjugated
5
5
5
5
5
5
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
67
50
44
9
8
5
2
1
1
1
Supplier
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Catalog No. ABIN357856
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN357855 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 3

Top referenced anti-DAAM1 antibody for Enzyme Immunoassay

Similar anti-DAAM1 Antibodies

Application / Reactivity Human
ELISA 50 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunohistochemistry (IHC) 44 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 68 Antibodies

General

Antigen Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) Antibodies
Epitope C-Term, N-Term
(34), (11), (8), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Reactivity Human
(75), (13), (13), (3), (3), (2), (2), (1), (1)
Host Rabbit
(69), (6)
Clonality
Polyclonal
Conjugate This DAAM1 antibody is un-conjugated
(5), (5), (5), (5), (5), (5)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(67), (50), (44), (9), (8), (5), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-DAAM1 Antibody

Target Details DAAM1 Application Details Handling References for anti-DAAM1 Antibody (ABIN357856) Images
Specificity This antibody detects DAAM1 (C-term).
Purification Protein A Chromatography followed by peptide affinity purification.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DAAM1.
Isotype Ig

Target Details DAAM1

Product Details anti-DAAM1 Antibody Application Details Handling References for anti-DAAM1 Antibody (ABIN357856) Images back to top
Antigen
Alternative Name DAAM1 (DAAM1 Antibody Abstract)
Background Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. DAAM1 contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by DAAM1, which is thought to function as a scaffolding protein.Synonyms: DAAM-1, Disheveled-associated activator of morphogenesis 1, FLJ41657, KIAA0666
Molecular Weight 123473 Da
Gene ID 23002, 9606
UniProt Q9Y4D1
Pathways WNT Signaling

Application Details

Product Details anti-DAAM1 Antibody Target Details DAAM1 Handling References for anti-DAAM1 Antibody (ABIN357856) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/50-1/100. Immunohistochemistry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DAAM1 Antibody Target Details DAAM1 Application Details References for anti-DAAM1 Antibody (ABIN357856) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-DAAM1 Antibody (ABIN357856)

Product Details anti-DAAM1 Antibody Target Details DAAM1 Application Details Handling Images back to top
Background publications

Liu, Sato, Khadka, Bharti, Diaz, Runnels, Habas: "Mechanism of activation of the Formin protein Daam1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 1, pp. 210-5, 2008

Yamashita, Higashi, Suetsugu, Sato, Ikeda, Shirakawa, Kita, Takenawa, Horiuchi, Fukai, Nureki: "Crystal structure of human DAAM1 formin homology 2 domain." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 12, Issue 11, pp. 1255-65, 2007

Lu, Meng, Poy, Maiti, Goode, Eck: "Structure of the FH2 domain of Daam1: implications for formin regulation of actin assembly." in: Journal of molecular biology, Vol. 369, Issue 5, pp. 1258-69, 2007

Images

Product Details anti-DAAM1 Antibody Target Details DAAM1 Application Details Handling References for anti-DAAM1 Antibody (ABIN357856) back to top
Supplier Images
 image for anti-DAAM1 antibody (Dishevelled Associated Activator of Morphogenesis 1) (C-Term) (ABIN357856) anti-Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) (C-Term), (N-Term) antibody
 image for anti-DAAM1 antibody (Dishevelled Associated Activator of Morphogenesis 1) (C-Term) (ABIN357856) anti-Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) (C-Term), (N-Term) antibody (Image 2)